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C5D8P2

- SYI_GEOSW

UniProt

C5D8P2 - SYI_GEOSW

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Geobacillus sp. (strain WCH70)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 41 (01 Oct 2014)
      Sequence version 1 (28 Jul 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei552 – 5521Aminoacyl-adenylateUniRule annotation
    Binding sitei596 – 5961ATPUniRule annotation
    Metal bindingi890 – 8901ZincUniRule annotation
    Metal bindingi893 – 8931ZincUniRule annotation
    Metal bindingi910 – 9101ZincUniRule annotation
    Metal bindingi913 – 9131ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciGSP471223:GH2C-1123-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:GWCH70_1039
    OrganismiGeobacillus sp. (strain WCH70)
    Taxonomic identifieri471223 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
    ProteomesiUP000002386: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 923923Isoleucine--tRNA ligasePRO_1000216236Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi471223.GWCH70_1039.

    Structurei

    3D structure databases

    ProteinModelPortaliC5D8P2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi593 – 5975"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    C5D8P2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYKETLLMP QTEFPMRGNL PKREPEIQKK WEEMDIYRKV QERTKGRPLF    50
    VLHDGPPYAN GDIHMGHALN KILKDIIVRY KSMSGYCAPY VPGWDTHGLP 100
    IETALTKKGV DRKSMSVAEF RKLCEQYAYE QINNQREQFK RLGVRGDWEN 150
    PYITLKPEYE AQQIKVFGEM AKKGLIYKGL KPVYWSPSSE SALAEAEIEY 200
    KDKRSPSIYV AFPVKDGKGV LDGDEKIVIW TTTPWTIPAN LAIAVHPDLD 250
    YQVVETNGAK YVVAAALLES VAKEIGWDEV TVVKTIKGKD LEYVVAKHPF 300
    YDRDSLVICG EHVTTDAGTG CVHTAPGHGE DDFIVGQKYG LDVLCPVDER 350
    GYMTSEAPGF EGLFYDEANK AITQKLEEVG ALLKLSFITH SYPHDWRTKK 400
    PTIFRATTQW FASIDKIRGE LLQAIKETKW IPEWGEIRIH NMIRDRGDWC 450
    ISRQRAWGVP IPVFYGENGE PIITDETIEH VSNLFRQYGS NVWFEREAKD 500
    LLPEGFTHPS SPNGIFTKEI DIMDVWFDSG SSHQAVLVER DDLQRPADLY 550
    LEGSDQYRGW FNSSLSTAVA VTGKAPYKAV LSHGFVLDGE GRKMSKSLGN 600
    VVVPAKVMEQ LGADILRLWV ASVDYQADVR ISDSILKQVA EVYRKIRNTF 650
    RFMLGNLFDF NPETDAVPVN ELREVDRYMI VKLNHLIENV KHAYETYDFA 700
    SIYHDVNNFC TVDLSAFYLD FAKDILYIEA PNDRARRSIQ TVLYETVVAL 750
    TKLVAPILPH TAEEVWEHIP NRKEKEESVQ LVDMPETINI DEEDAIVAKW 800
    DAFMNLRDDV LKALEVARNE KVIGKSLTAS VTVYPTKEAR QLLGSIEEDL 850
    KQLFIVSEFT IADDYEHAPE DAQKLANVAV IVKPAEGETC ERCWVVTPEV 900
    GKDADHPTLC PRCAHIVKEH YSA 923
    Length:923
    Mass (Da):104,948
    Last modified:July 28, 2009 - v1
    Checksum:i631E16D4758054CC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001638 Genomic DNA. Translation: ACS23899.1.
    RefSeqiWP_015863374.1. NC_012793.1.
    YP_002949165.1. NC_012793.1.

    Genome annotation databases

    EnsemblBacteriaiACS23899; ACS23899; GWCH70_1039.
    GeneIDi7976822.
    KEGGigwc:GWCH70_1039.
    PATRICi21971004. VBIGeoSp101709_1117.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001638 Genomic DNA. Translation: ACS23899.1 .
    RefSeqi WP_015863374.1. NC_012793.1.
    YP_002949165.1. NC_012793.1.

    3D structure databases

    ProteinModelPortali C5D8P2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 471223.GWCH70_1039.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACS23899 ; ACS23899 ; GWCH70_1039 .
    GeneIDi 7976822.
    KEGGi gwc:GWCH70_1039.
    PATRICi 21971004. VBIGeoSp101709_1117.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci GSP471223:GH2C-1123-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: WCH70.

    Entry informationi

    Entry nameiSYI_GEOSW
    AccessioniPrimary (citable) accession number: C5D8P2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: July 28, 2009
    Last modified: October 1, 2014
    This is version 41 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3