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C5D8L9

- C5D8L9_GEOSW

UniProt

C5D8L9 - C5D8L9_GEOSW

Protein

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase

Gene

murE

Organism
Geobacillus sp. (strain WCH70)
Status
Unreviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 46 (01 Oct 2014)
      Sequence version 1 (28 Jul 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.UniRule annotation

    Catalytic activityi

    ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei30 – 301UDP-MurNAc-L-Ala-D-GluUniRule annotation
    Binding sitei149 – 1491UDP-MurNAc-L-Ala-D-GluUniRule annotation
    Binding sitei177 – 1771UDP-MurNAc-L-Ala-D-GluUniRule annotation
    Binding sitei185 – 1851UDP-MurNAc-L-Ala-D-GluUniRule annotation
    Binding sitei383 – 3831Meso-diaminopimelateUniRule annotation
    Binding sitei457 – 4571Meso-diaminopimelate; via carbonyl oxygenUniRule annotation
    Binding sitei461 – 4611Meso-diaminopimelateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi108 – 1147ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. cell division Source: UniProtKB-KW
    3. peptidoglycan biosynthetic process Source: UniProtKB-HAMAP
    4. regulation of cell shape Source: UniProtKB-KW

    Keywords - Molecular functioni

    LigaseUniRule annotationSAAS annotation

    Keywords - Biological processi

    Cell cycle, Cell divisionUniRule annotationSAAS annotation, Cell shape, Cell wall biogenesis/degradationUniRule annotationSAAS annotation, Peptidoglycan synthesisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciGSP471223:GH2C-1100-MONOMER.
    UniPathwayiUPA00219.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligaseUniRule annotation (EC:6.3.2.13UniRule annotation)
    Alternative name(s):
    Meso-A2pm-adding enzymeUniRule annotation
    Meso-diaminopimelate-adding enzymeUniRule annotation
    UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligaseUniRule annotation
    UDP-MurNAc-tripeptide synthetaseUniRule annotation
    UDP-N-acetylmuramyl-tripeptide synthetaseUniRule annotation
    Gene namesi
    Name:murEUniRule annotation
    Ordered Locus Names:GWCH70_1016Imported
    OrganismiGeobacillus sp. (strain WCH70)Imported
    Taxonomic identifieri471223 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
    ProteomesiUP000002386: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    PTM / Processingi

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei217 – 2171N6-carboxylysineUniRule annotation

    Post-translational modificationi

    Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP.UniRule annotation

    Interactioni

    Protein-protein interaction databases

    STRINGi471223.GWCH70_1016.

    Structurei

    3D structure databases

    ProteinModelPortaliC5D8L9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni150 – 1512UDP-MurNAc-L-Ala-D-Glu bindingUniRule annotation
    Regioni407 – 4104Meso-diaminopimelate bindingUniRule annotation

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi407 – 4104Meso-diaminopimelate recognition motifUniRule annotation

    Sequence similaritiesi

    Belongs to the MurCDEF family. MurE subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0769.
    HOGENOMiHOG000268118.
    KOiK01928.
    OMAiKIAVDYA.
    OrthoDBiEOG6PKFCR.

    Family and domain databases

    Gene3Di3.40.1190.10. 1 hit.
    3.40.1390.10. 1 hit.
    3.90.190.20. 1 hit.
    HAMAPiMF_00208. MurE.
    InterProiIPR004101. Mur_ligase_C.
    IPR013221. Mur_ligase_cen.
    IPR000713. Mur_ligase_N.
    IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase.
    [Graphical view]
    PfamiPF01225. Mur_ligase. 1 hit.
    PF02875. Mur_ligase_C. 1 hit.
    PF08245. Mur_ligase_M. 1 hit.
    [Graphical view]
    SUPFAMiSSF53244. SSF53244. 1 hit.
    SSF53623. SSF53623. 1 hit.
    SSF63418. SSF63418. 1 hit.
    TIGRFAMsiTIGR01085. murE. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    C5D8L9-1 [UniParc]FASTAAdd to Basket

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    MKLQTLLSYL HDFTTYVGEN PTITSIEMDS RQVKNGALFI CIKGFTVDGH    50
    DFAKQAVENG AVAVIAERPL DVDVPVIVVR DSRRAMAMLA DAFYGQPTHR 100
    LHLIGVTGTN GKTTTTHIIE HIARKANKKT GLIGTVHIKI GDHTYPTKNT 150
    TPESLLLQRT FKQMVDEGVD VAVMEVSSHA LHMGRVHGCD YDVAVFTNLT 200
    QDHLDYHGTM EEYRNAKGLL FAQLGNRYDH ERPKFAVLNN DDPASKYYKH 250
    MTAASVITYG IETDSDIMAK KIDIVPSGMS FDLITPNGTV RVKTRLIGLF 300
    NVYNLLAAVS ACLVSGIPLP VIVEAIEEIS GVAGRFETVD EGQNFTVIVD 350
    YAHTPDSLEN VLKTIRQFAQ KKVYVVVGCG GDRDRTKRPL MAKTAVKYAD 400
    VAIFTSDNPR SEDPEQILRD MEAGVPGERY VTIVDRKEAI YYAIEQAQEG 450
    DIILIAGKGH ETYQIIGDRV IDFDDRLVAR EAIKERKNIC 490
    Length:490
    Mass (Da):54,388
    Last modified:July 28, 2009 - v1
    Checksum:i51130A205C60C782
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001638 Genomic DNA. Translation: ACS23876.1.
    RefSeqiWP_015863352.1. NC_012793.1.
    YP_002949142.1. NC_012793.1.

    Genome annotation databases

    EnsemblBacteriaiACS23876; ACS23876; GWCH70_1016.
    GeneIDi7976802.
    KEGGigwc:GWCH70_1016.
    PATRICi21970958. VBIGeoSp101709_1094.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001638 Genomic DNA. Translation: ACS23876.1 .
    RefSeqi WP_015863352.1. NC_012793.1.
    YP_002949142.1. NC_012793.1.

    3D structure databases

    ProteinModelPortali C5D8L9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 471223.GWCH70_1016.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACS23876 ; ACS23876 ; GWCH70_1016 .
    GeneIDi 7976802.
    KEGGi gwc:GWCH70_1016.
    PATRICi 21970958. VBIGeoSp101709_1094.

    Phylogenomic databases

    eggNOGi COG0769.
    HOGENOMi HOG000268118.
    KOi K01928.
    OMAi KIAVDYA.
    OrthoDBi EOG6PKFCR.

    Enzyme and pathway databases

    UniPathwayi UPA00219 .
    BioCyci GSP471223:GH2C-1100-MONOMER.

    Family and domain databases

    Gene3Di 3.40.1190.10. 1 hit.
    3.40.1390.10. 1 hit.
    3.90.190.20. 1 hit.
    HAMAPi MF_00208. MurE.
    InterProi IPR004101. Mur_ligase_C.
    IPR013221. Mur_ligase_cen.
    IPR000713. Mur_ligase_N.
    IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase.
    [Graphical view ]
    Pfami PF01225. Mur_ligase. 1 hit.
    PF02875. Mur_ligase_C. 1 hit.
    PF08245. Mur_ligase_M. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53244. SSF53244. 1 hit.
    SSF53623. SSF53623. 1 hit.
    SSF63418. SSF63418. 1 hit.
    TIGRFAMsi TIGR01085. murE. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: WCH70Imported.

    Entry informationi

    Entry nameiC5D8L9_GEOSW
    AccessioniPrimary (citable) accession number: C5D8L9
    Entry historyi
    Integrated into UniProtKB/TrEMBL: July 28, 2009
    Last sequence update: July 28, 2009
    Last modified: October 1, 2014
    This is version 46 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3