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C5D8L9

- C5D8L9_GEOSW

UniProt

C5D8L9 - C5D8L9_GEOSW

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Protein
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
Gene
murE, GWCH70_1016
Organism
Geobacillus sp. (strain WCH70)
Status
Unreviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity.UniRule annotation

Catalytic activityi

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei30 – 301UDP-MurNAc-L-Ala-D-Glu By similarityUniRule annotation
Binding sitei149 – 1491UDP-MurNAc-L-Ala-D-Glu By similarityUniRule annotation
Binding sitei177 – 1771UDP-MurNAc-L-Ala-D-Glu By similarityUniRule annotation
Binding sitei185 – 1851UDP-MurNAc-L-Ala-D-Glu By similarityUniRule annotation
Binding sitei383 – 3831Meso-diaminopimelate By similarityUniRule annotation
Binding sitei457 – 4571Meso-diaminopimelate; via carbonyl oxygen By similarityUniRule annotation
Binding sitei461 – 4611Meso-diaminopimelate By similarityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi108 – 1147ATP By similarityUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. peptidoglycan biosynthetic process Source: UniProtKB-HAMAP
  4. regulation of cell shape Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationSAAS annotations

Keywords - Biological processi

Cell cycle, Cell divisionUniRule annotationSAAS annotations, Cell shape, Cell wall biogenesis/degradationUniRule annotationSAAS annotations, Peptidoglycan synthesisUniRule annotationSAAS annotations

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotations, Nucleotide-binding

Enzyme and pathway databases

BioCyciGSP471223:GH2C-1100-MONOMER.
UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligaseUniRule annotation (EC:6.3.2.13UniRule annotation)
Alternative name(s):
Meso-A2pm-adding enzyme
Meso-diaminopimelate-adding enzyme
UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
UDP-MurNAc-tripeptide synthetase
UDP-N-acetylmuramyl-tripeptide synthetase
Gene namesi
Name:murEUniRule annotation
Ordered Locus Names:GWCH70_1016Imported
OrganismiGeobacillus sp. (strain WCH70)Imported
Taxonomic identifieri471223 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
ProteomesiUP000002386: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotationSAAS annotations

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotations

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei217 – 2171N6-carboxylysine By similarityUniRule annotation

Post-translational modificationi

Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity.UniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi471223.GWCH70_1016.

Structurei

3D structure databases

ProteinModelPortaliC5D8L9.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni150 – 1512UDP-MurNAc-L-Ala-D-Glu binding By similarityUniRule annotation
Regioni407 – 4104Meso-diaminopimelate binding By similarityUniRule annotation

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi407 – 4104Meso-diaminopimelate recognition motif By similarityUniRule annotation

Sequence similaritiesi

Belongs to the MurCDEF family. MurE subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0769.
HOGENOMiHOG000268118.
KOiK01928.
OMAiKIAVDYA.
OrthoDBiEOG6PKFCR.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.1390.10. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_00208. MurE.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase.
[Graphical view]
PfamiPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
SSF63418. SSF63418. 1 hit.
TIGRFAMsiTIGR01085. murE. 1 hit.

Sequencei

Sequence statusi: Complete.

C5D8L9-1 [UniParc]FASTAAdd to Basket

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MKLQTLLSYL HDFTTYVGEN PTITSIEMDS RQVKNGALFI CIKGFTVDGH    50
DFAKQAVENG AVAVIAERPL DVDVPVIVVR DSRRAMAMLA DAFYGQPTHR 100
LHLIGVTGTN GKTTTTHIIE HIARKANKKT GLIGTVHIKI GDHTYPTKNT 150
TPESLLLQRT FKQMVDEGVD VAVMEVSSHA LHMGRVHGCD YDVAVFTNLT 200
QDHLDYHGTM EEYRNAKGLL FAQLGNRYDH ERPKFAVLNN DDPASKYYKH 250
MTAASVITYG IETDSDIMAK KIDIVPSGMS FDLITPNGTV RVKTRLIGLF 300
NVYNLLAAVS ACLVSGIPLP VIVEAIEEIS GVAGRFETVD EGQNFTVIVD 350
YAHTPDSLEN VLKTIRQFAQ KKVYVVVGCG GDRDRTKRPL MAKTAVKYAD 400
VAIFTSDNPR SEDPEQILRD MEAGVPGERY VTIVDRKEAI YYAIEQAQEG 450
DIILIAGKGH ETYQIIGDRV IDFDDRLVAR EAIKERKNIC 490
Length:490
Mass (Da):54,388
Last modified:July 28, 2009 - v1
Checksum:i51130A205C60C782
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001638 Genomic DNA. Translation: ACS23876.1.
RefSeqiWP_015863352.1. NC_012793.1.
YP_002949142.1. NC_012793.1.

Genome annotation databases

EnsemblBacteriaiACS23876; ACS23876; GWCH70_1016.
GeneIDi7976802.
KEGGigwc:GWCH70_1016.
PATRICi21970958. VBIGeoSp101709_1094.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001638 Genomic DNA. Translation: ACS23876.1 .
RefSeqi WP_015863352.1. NC_012793.1.
YP_002949142.1. NC_012793.1.

3D structure databases

ProteinModelPortali C5D8L9.
ModBasei Search...

Protein-protein interaction databases

STRINGi 471223.GWCH70_1016.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACS23876 ; ACS23876 ; GWCH70_1016 .
GeneIDi 7976802.
KEGGi gwc:GWCH70_1016.
PATRICi 21970958. VBIGeoSp101709_1094.

Phylogenomic databases

eggNOGi COG0769.
HOGENOMi HOG000268118.
KOi K01928.
OMAi KIAVDYA.
OrthoDBi EOG6PKFCR.

Enzyme and pathway databases

UniPathwayi UPA00219 .
BioCyci GSP471223:GH2C-1100-MONOMER.

Family and domain databases

Gene3Di 3.40.1190.10. 1 hit.
3.40.1390.10. 1 hit.
3.90.190.20. 1 hit.
HAMAPi MF_00208. MurE.
InterProi IPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase.
[Graphical view ]
Pfami PF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view ]
SUPFAMi SSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
SSF63418. SSF63418. 1 hit.
TIGRFAMsi TIGR01085. murE. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: WCH70Imported.

Entry informationi

Entry nameiC5D8L9_GEOSW
AccessioniPrimary (citable) accession number: C5D8L9
Entry historyi
Integrated into UniProtKB/TrEMBL: July 28, 2009
Last sequence update: July 28, 2009
Last modified: September 3, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

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