C5D7A6 (PRPE_GEOSW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bis(5'-nucleosyl)-tetraphosphatase prpE [asymmetrical]
EC=3.6.1.17

Alternative name(s):
Ap4A hydrolase
Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase
Short name=Diadenosine tetraphosphatase

Gene names

Name:	prpE
Ordered Locus Names:	GWCH70_0778

Organism

Geobacillus sp. (strain WCH70) [Complete proteome] [HAMAP]

Taxonomic identifier

471223 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Asymmetrically hydrolyzes Ap4p to yield AMP and ATP By similarity. HAMAP MF_01443
Catalytic activity	P1,P(4)-bis(5'-guanosyl) tetraphosphate + H2O = GTP + GMP. HAMAP MF_01443
Cofactor	Nickel By similarity. HAMAP MF_01443
Sequence similarities	Belongs to the prpE family.

Ontologies

Keywords
Ligand	GTP-binding Nickel Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity Inferred from electronic annotation. Source: EC nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	Bis(5'-nucleosyl)-tetraphosphatase prpE [asymmetrical] HAMAP MF_01443		PRO_1000215291

Sequences

	Sequence		Length	Mass (Da)
	C5D7A6 [UniParc]. Last modified July 28, 2009. Version 1. Checksum: 08E4A07F4729732A	FASTA	245	27,938
	10 20 30 40 50 60 MKIDIIGDIH GCYSEFVKLT KKLGYEWDSG IPLHPDGRKL GFVGDLTDRG PQSLQTVEVV 70 80 90 100 110 120 YSLVERESAY YVPGNHCNKL YRFFLGRNVQ ITHGLETTVE EYRALPPSEQ AMIRKKFMRL 130 140 150 160 170 180 YEAAPLYAQL DNGRLIIAHA GLRRDYIGRN DKKVQTFVLY GDITGETNPD GTPVRRDWAK 190 200 210 220 230 240 RYKGSAWIVY GHTPVKQPRI INRTINIDTG CVFGGALTAF RYPEIETISV PSSLPYVPEK FRTFD

References

[1]

"Complete sequence of chromosome of Geopacillus sp. WCH70." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Brumm P., Mead D.A., Richardson P. Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WCH70.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001638 Genomic DNA. Translation: ACS23664.1.
RefSeq	YP_002948930.1. NC_012793.1.
3D structure databases
ProteinModelPortal	C5D7A6.
ModBase	Search...
Protein-protein interaction databases
STRING	C5D7A6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7977726.
GenomeReviews	Gene locus GWCH70_0778 in contig CP001638_GR.
KEGG	gwc:GWCH70_0778.
PATRIC	21970449. VBIGeoSp101709_0841.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	YVPGNHC.
ProtClustDB	PRK13625.
Family and domain databases
HAMAP	MF_01443. PrpE. [Tree]
InterPro	IPR023937. Bis(5'-nucleosyl)-tetraP_PrpE. IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR00114. STPHPHTASE.
ProtoNet	Search...

Entry information

Entry name

PRPE_GEOSW

Accession

Primary (citable) accession number: C5D7A6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	July 28, 2009
Last modified:	January 25, 2012
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program