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C5D729

- DAPF_GEOSW

UniProt

C5D729 - DAPF_GEOSW

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Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Geobacillus sp. (strain WCH70)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

Catalytic activityi

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei14 – 141SubstrateUniRule annotation
Binding sitei49 – 491SubstrateUniRule annotation
Binding sitei67 – 671SubstrateUniRule annotation
Active sitei76 – 761Proton donor/acceptorUniRule annotation
Binding sitei166 – 1661SubstrateUniRule annotation
Sitei168 – 1681Important for catalytic activityUniRule annotation
Binding sitei199 – 1991SubstrateUniRule annotation
Sitei217 – 2171Important for catalytic activityUniRule annotation
Active sitei226 – 2261Proton donor/acceptorUniRule annotation

GO - Molecular functioni

  1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Amino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

BioCyciGSP471223:GH2C-3001-MONOMER.
UniPathwayiUPA00034; UER00025.

Names & Taxonomyi

Protein namesi
Recommended name:
Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
Short name:
DAP epimeraseUniRule annotation
Gene namesi
Name:dapFUniRule annotation
Ordered Locus Names:GWCH70_2888
OrganismiGeobacillus sp. (strain WCH70)
Taxonomic identifieri471223 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
ProteomesiUP000002386: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 284284Diaminopimelate epimerasePRO_1000204062Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi76 ↔ 226

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi471223.GWCH70_2888.

Structurei

3D structure databases

ProteinModelPortaliC5D729.
SMRiC5D729. Positions 1-284.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni11 – 122Substrate bindingUniRule annotation
Regioni76 – 783Substrate bindingUniRule annotation
Regioni217 – 2182Substrate bindingUniRule annotation
Regioni227 – 2282Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the diaminopimelate epimerase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0253.
HOGENOMiHOG000220466.
KOiK01778.
OMAiKMRIFNN.
OrthoDBiEOG6ND0M5.

Family and domain databases

HAMAPiMF_00197. DAP_epimerase.
InterProiIPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view]
PANTHERiPTHR31689. PTHR31689. 1 hit.
PfamiPF01678. DAP_epimerase. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00652. DapF. 1 hit.
PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C5D729-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNKFSFTKMH GLGNSYIYVN MFEETIPESL LSSLAVKVSN VNTGIGADGM
60 70 80 90 100
ILICPSEVAP VKMRIFNSDG SEGKNCGNGL RCVAKYAYEH GMVKDRSFFI
110 120 130 140 150
ETLSGLVKAE VTVENGAVTN VTIDMGKPRL KRSEIPMIGL DAERVVAEPF
160 170 180 190 200
EVDGKQYEIT AVSMGNPHVI FYVDDITTAP VTTLGPIVEK DERFPEGVNV
210 220 230 240 250
EFVEVVNDHE LHFRVWERGS GVTQACGTGA CAAVVASVLN GKTARNKETV
260 270 280
VHLAGGDLII TWTDEGNVRM TGPAETICTG VYYY
Length:284
Mass (Da):30,842
Last modified:July 28, 2009 - v1
Checksum:iA8D122BF91C9958F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001638 Genomic DNA. Translation: ACS25568.1.
RefSeqiWP_015864961.1. NC_012793.1.
YP_002950834.1. NC_012793.1.

Genome annotation databases

EnsemblBacteriaiACS25568; ACS25568; GWCH70_2888.
GeneIDi7978111.
KEGGigwc:GWCH70_2888.
PATRICi21974895. VBIGeoSp101709_3029.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001638 Genomic DNA. Translation: ACS25568.1 .
RefSeqi WP_015864961.1. NC_012793.1.
YP_002950834.1. NC_012793.1.

3D structure databases

ProteinModelPortali C5D729.
SMRi C5D729. Positions 1-284.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 471223.GWCH70_2888.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACS25568 ; ACS25568 ; GWCH70_2888 .
GeneIDi 7978111.
KEGGi gwc:GWCH70_2888.
PATRICi 21974895. VBIGeoSp101709_3029.

Phylogenomic databases

eggNOGi COG0253.
HOGENOMi HOG000220466.
KOi K01778.
OMAi KMRIFNN.
OrthoDBi EOG6ND0M5.

Enzyme and pathway databases

UniPathwayi UPA00034 ; UER00025 .
BioCyci GSP471223:GH2C-3001-MONOMER.

Family and domain databases

HAMAPi MF_00197. DAP_epimerase.
InterProi IPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view ]
PANTHERi PTHR31689. PTHR31689. 1 hit.
Pfami PF01678. DAP_epimerase. 2 hits.
[Graphical view ]
TIGRFAMsi TIGR00652. DapF. 1 hit.
PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: WCH70.

Entry informationi

Entry nameiDAPF_GEOSW
AccessioniPrimary (citable) accession number: C5D729
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: October 29, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3