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C5D6D7 (SYL_GEOSW) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:GWCH70_2764
OrganismGeobacillus sp. (strain WCH70) [Complete proteome] [HAMAP]
Taxonomic identifier471223 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Protein attributes

Sequence length805 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 805805Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000202221

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif576 – 5805"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5791ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
C5D6D7 [UniParc].

Last modified July 28, 2009. Version 1.
Checksum: EC08CF540D2FB8D9

FASTA80592,561
        10         20         30         40         50         60 
MSFNHREIEK KWQEYWEKNK TFKTVDDDDK PKFYVLDMFP YPSGAGLHVG HPEGYTATDI 

        70         80         90        100        110        120 
LARMKRMQGY NVLHPMGWDA FGLPAEQYAL DTGNDPAEFT EKNINTFRRQ IKSLGFSYDW 

       130        140        150        160        170        180 
DREINTTDPN YYKWTQWIFL KLYEKGLAYM DEVPVNWCPA LGTVLANEEV IDGKSERGGH 

       190        200        210        220        230        240 
PVIRKPMKQW LLRITAYADR LLEDLEELDW PESVKEMQRN WIGRSEGAEI RFEVDGHDET 

       250        260        270        280        290        300 
FTVFTTRPDT LFGATYTVLA PEHPLVEKIT TPEQKAAVEA YLDAIKSKSD LERTDLAKEK 

       310        320        330        340        350        360 
TGVFTGAYAI HPVTGEKLPI WIADYVLMSY GTGAIMAVPA HDERDYEFAK KFNLPIKEVV 

       370        380        390        400        410        420 
AGGDIAKEAY TGDGEHINSD FLNGLNKEEA IKKMIEWLEA NGKGQKKVTY RLRDWLFSRQ 

       430        440        450        460        470        480 
RYWGEPIPII HWEDGTMTPV PEEELPLKLP KTDKIKPTGT GESPLANIEE WVNVVDPKTG 

       490        500        510        520        530        540 
KKGRRETNTM PQWAGSCWYY LRYIDPHNDK QLADPEKLKK WLPVDVYIGG AEHAVLHLLY 

       550        560        570        580        590        600 
ARFWHKFLYD IGVVPTKEPF QKLFNQGMIL GENNEKMSKS RGNVVNPDDI IESHGADTLR 

       610        620        630        640        650        660 
LYEMFMGPLE ASIAWSTKGL DGARRFLDRV WRLFVDENGN LNPKIVDNPE TDTLERVYHQ 

       670        680        690        700        710        720 
TVKKVTEDYE ALRFNTAISQ LMVFINEAYK APVLPKEYME GFVKLLSPVC PHIGEELWEK 

       730        740        750        760        770        780 
LGHNNTIAYE PWPTYDESKL VEEEVEIVVQ VNGKVRAKLR VPADISKEKL EQLAMEDEKI 

       790        800 
KEHIAGKTVR KVITVPGKLV NIVAN 

« Hide

References

[1]"Complete sequence of chromosome of Geopacillus sp. WCH70."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. expand/collapse author list , Brumm P., Mead D.A., Richardson P.
Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: WCH70.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001638 Genomic DNA. Translation: ACS25453.1.
RefSeqYP_002950719.1. NC_012793.1.

3D structure databases

ProteinModelPortalC5D6D7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING471223.GWCH70_2764.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACS25453; ACS25453; GWCH70_2764.
GeneID7977987.
KEGGgwc:GWCH70_2764.
PATRIC21974594. VBIGeoSp101709_2898.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycGSP471223:GH2C-2860-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_GEOSW
AccessionPrimary (citable) accession number: C5D6D7
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: June 11, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries