C5D627 (SYFA_GEOSW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha subunit
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha subunit
Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	GWCH70_2649

Organism

Geobacillus sp. (strain WCH70) [Complete proteome] [HAMAP]

Taxonomic identifier

471223 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP-Rule MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity.
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Phenylalanine--tRNA ligase alpha subunit HAMAP-Rule MF_00281		PRO_1000204829
Sites
	Metal binding	256	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	C5D627 [UniParc]. Last modified July 28, 2009. Version 1. Checksum: BB605E646A5E3955	FASTA	344	39,011
	10 20 30 40 50 60 MKERLQQLQQ EALEKIEQAN DLKALNEVRV AYLGKKGPIT EVLRGMGALS PEERPVIGAL 70 80 90 100 110 120 ANEVRQAIQS ALEAKQAKLE QEEVEKKLAA EAIDVTLPGR PVKRGNHHPL TRVIEEIEDL 130 140 150 160 170 180 FIGMGYTIAE GPEVEKDYYN FEALNLPKGH PARDMQDSFY ITEEILLRTH TSPVQARTME 190 200 210 220 230 240 KHKGRGPVKI ICPGKVYRRD NDDATHSHQF TQIEGLVVDE NIRMSDLKGT LREFARKMFG 250 260 270 280 290 300 KDREIRFRPS FFPFTEPSVE VDVSCFHCGG HGCSVCKGTG WIEILGAGMV HPNVLEMAGF 310 320 330 340 DSKKYTGFAF GMGPERIAML KYGIDDIRHF YQNDIRFLQQ FHRV

References

[1]

"Complete sequence of chromosome of Geopacillus sp. WCH70." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Brumm P., Mead D.A., Richardson P. Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WCH70.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001638 Genomic DNA. Translation: ACS25343.1.
RefSeq	YP_002950609.1. NC_012793.1.
3D structure databases
ProteinModelPortal	C5D627.
SMR	C5D627. Positions 82-344.
ModBase	Search...
Protein-protein interaction databases
STRING	471223.GWCH70_2649.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACS25343; ACS25343; GWCH70_2649.
GeneID	7978310.
KEGG	gwc:GWCH70_2649.
PATRIC	21974362. VBIGeoSp101709_2782.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0016.
HOGENOM	HOG000242675.
KO	K01889.
OMA	LTHTPMF.
ProtClustDB	PRK00488.
Enzyme and pathway databases
BioCyc	GSP471223:GH2C-2778-MONOMER.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1.
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_ligase_II_N. IPR022911. Phe_tRNA_ligase_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. IPR010978. tRNA-bd_arm. [Graphical view]
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00468. pheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_GEOSW

Accession

Primary (citable) accession number: C5D627

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	July 28, 2009
Last modified:	May 1, 2013
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program