Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

C5D5M0

- LEU1_GEOSW

UniProt

C5D5M0 - LEU1_GEOSW

Protein

2-isopropylmalate synthase

Gene

leuA

Organism
Geobacillus sp. (strain WCH70)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 40 (01 Oct 2014)
      Sequence version 1 (28 Jul 2009)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).UniRule annotation

    Catalytic activityi

    Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA.UniRule annotation

    Pathwayi

    GO - Molecular functioni

    1. 2-isopropylmalate synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. leucine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

    Enzyme and pathway databases

    BioCyciGSP471223:GH2C-2688-MONOMER.
    UniPathwayiUPA00048; UER00070.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2-isopropylmalate synthaseUniRule annotation (EC:2.3.3.13UniRule annotation)
    Alternative name(s):
    Alpha-IPM synthaseUniRule annotation
    Alpha-isopropylmalate synthaseUniRule annotation
    Gene namesi
    Name:leuAUniRule annotation
    Ordered Locus Names:GWCH70_2593
    OrganismiGeobacillus sp. (strain WCH70)
    Taxonomic identifieri471223 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
    ProteomesiUP000002386: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 5155152-isopropylmalate synthasePRO_1000213314Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi471223.GWCH70_2593.

    Structurei

    3D structure databases

    ProteinModelPortaliC5D5M0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0119.
    HOGENOMiHOG000046859.
    KOiK01649.
    OMAiRKINIFD.
    OrthoDBiEOG6CGCF3.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01025. LeuA_type1.
    InterProiIPR013709. 2-isopropylmalate_synth_dimer.
    IPR002034. AIPM/Hcit_synth_CS.
    IPR013785. Aldolase_TIM.
    IPR005671. LeuA_bact_synth.
    IPR000891. PYR_CT.
    [Graphical view]
    PfamiPF00682. HMGL-like. 1 hit.
    PF08502. LeuA_dimer. 1 hit.
    [Graphical view]
    SMARTiSM00917. LeuA_dimer. 1 hit.
    [Graphical view]
    SUPFAMiSSF110921. SSF110921. 1 hit.
    TIGRFAMsiTIGR00973. leuA_bact. 1 hit.
    PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
    PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
    PS50991. PYR_CT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    C5D5M0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRKINIFDTT LRDGEQSAGI NLNLQEKLEI ARQLERLGVD IIEAGFPASS    50
    KGDFQAVKQI AETIKTCSVT GLARSVQSDI DAAWEALKGG AEPRLHLFIA 100
    TSPIHMKYKL QMTPEQVIET AVESVKYAKR YFPIVQWSAE DACRSELPFL 150
    AKIITEVIKA GATVINIPDT VGYITPKEYG NIFTFLSNNV PNIEKVSLSA 200
    HCHDDLGMAV ANSLAAIEHG ATQIEGTING IGERAGNAAL EEIAVALYIR 250
    KDYYQAETRL NLQEIKRTSN LVSKLTGVVI PPNKAVIGKN AFAHESGIHQ 300
    DGVLKEKTTY EIISPELVGV QSNSMVLGKH SGRHALRNRV EELGYTLSDE 350
    EVNKLFVRFK ELADKKKDIT DDDLVALIFE EKFDHFKDFY QLSSLQVQYG 400
    TNQIPTAVVV LKDGQGNEIQ EAATGAGSVE ALYNTLERCF KTSVTLLDYR 450
    IESVSGGRDA LAQVFVKVRV NDIETSGRGT AQDVLEASAK AYINAVNRVF 500
    MIETMRAENQ KVAMQ 515
    Length:515
    Mass (Da):56,758
    Last modified:July 28, 2009 - v1
    Checksum:iBD59459DE3FDC9BB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001638 Genomic DNA. Translation: ACS25288.1.
    RefSeqiWP_015864699.1. NC_012793.1.
    YP_002950554.1. NC_012793.1.

    Genome annotation databases

    EnsemblBacteriaiACS25288; ACS25288; GWCH70_2593.
    GeneIDi7976353.
    KEGGigwc:GWCH70_2593.
    PATRICi21974248. VBIGeoSp101709_2726.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001638 Genomic DNA. Translation: ACS25288.1 .
    RefSeqi WP_015864699.1. NC_012793.1.
    YP_002950554.1. NC_012793.1.

    3D structure databases

    ProteinModelPortali C5D5M0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 471223.GWCH70_2593.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACS25288 ; ACS25288 ; GWCH70_2593 .
    GeneIDi 7976353.
    KEGGi gwc:GWCH70_2593.
    PATRICi 21974248. VBIGeoSp101709_2726.

    Phylogenomic databases

    eggNOGi COG0119.
    HOGENOMi HOG000046859.
    KOi K01649.
    OMAi RKINIFD.
    OrthoDBi EOG6CGCF3.

    Enzyme and pathway databases

    UniPathwayi UPA00048 ; UER00070 .
    BioCyci GSP471223:GH2C-2688-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01025. LeuA_type1.
    InterProi IPR013709. 2-isopropylmalate_synth_dimer.
    IPR002034. AIPM/Hcit_synth_CS.
    IPR013785. Aldolase_TIM.
    IPR005671. LeuA_bact_synth.
    IPR000891. PYR_CT.
    [Graphical view ]
    Pfami PF00682. HMGL-like. 1 hit.
    PF08502. LeuA_dimer. 1 hit.
    [Graphical view ]
    SMARTi SM00917. LeuA_dimer. 1 hit.
    [Graphical view ]
    SUPFAMi SSF110921. SSF110921. 1 hit.
    TIGRFAMsi TIGR00973. leuA_bact. 1 hit.
    PROSITEi PS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
    PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
    PS50991. PYR_CT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: WCH70.

    Entry informationi

    Entry nameiLEU1_GEOSW
    AccessioniPrimary (citable) accession number: C5D5M0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: July 28, 2009
    Last modified: October 1, 2014
    This is version 40 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3