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Protein

2-isopropylmalate synthase

Gene

leuA

Organism
Geobacillus sp. (strain WCH70)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).UniRule annotation

Catalytic activityi

Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA.UniRule annotation

Pathwayi

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Enzyme and pathway databases

BioCyciGSP471223:GH2C-2688-MONOMER.
UniPathwayiUPA00048; UER00070.

Names & Taxonomyi

Protein namesi
Recommended name:
2-isopropylmalate synthaseUniRule annotation (EC:2.3.3.13UniRule annotation)
Alternative name(s):
Alpha-IPM synthaseUniRule annotation
Alpha-isopropylmalate synthaseUniRule annotation
Gene namesi
Name:leuAUniRule annotation
Ordered Locus Names:GWCH70_2593
OrganismiGeobacillus sp. (strain WCH70)
Taxonomic identifieri471223 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
ProteomesiUP000002386 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5155152-isopropylmalate synthasePRO_1000213314Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi471223.GWCH70_2593.

Structurei

3D structure databases

ProteinModelPortaliC5D5M0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0119.
HOGENOMiHOG000046859.
KOiK01649.
OMAiWSVHCHN.
OrthoDBiEOG6CGCF3.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01025. LeuA_type1.
InterProiIPR013709. 2-isopropylmalate_synth_dimer.
IPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR005671. LeuA_bact_synth.
IPR000891. PYR_CT.
[Graphical view]
PfamiPF00682. HMGL-like. 1 hit.
PF08502. LeuA_dimer. 1 hit.
[Graphical view]
SMARTiSM00917. LeuA_dimer. 1 hit.
[Graphical view]
SUPFAMiSSF110921. SSF110921. 1 hit.
TIGRFAMsiTIGR00973. leuA_bact. 1 hit.
PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C5D5M0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKINIFDTT LRDGEQSAGI NLNLQEKLEI ARQLERLGVD IIEAGFPASS
60 70 80 90 100
KGDFQAVKQI AETIKTCSVT GLARSVQSDI DAAWEALKGG AEPRLHLFIA
110 120 130 140 150
TSPIHMKYKL QMTPEQVIET AVESVKYAKR YFPIVQWSAE DACRSELPFL
160 170 180 190 200
AKIITEVIKA GATVINIPDT VGYITPKEYG NIFTFLSNNV PNIEKVSLSA
210 220 230 240 250
HCHDDLGMAV ANSLAAIEHG ATQIEGTING IGERAGNAAL EEIAVALYIR
260 270 280 290 300
KDYYQAETRL NLQEIKRTSN LVSKLTGVVI PPNKAVIGKN AFAHESGIHQ
310 320 330 340 350
DGVLKEKTTY EIISPELVGV QSNSMVLGKH SGRHALRNRV EELGYTLSDE
360 370 380 390 400
EVNKLFVRFK ELADKKKDIT DDDLVALIFE EKFDHFKDFY QLSSLQVQYG
410 420 430 440 450
TNQIPTAVVV LKDGQGNEIQ EAATGAGSVE ALYNTLERCF KTSVTLLDYR
460 470 480 490 500
IESVSGGRDA LAQVFVKVRV NDIETSGRGT AQDVLEASAK AYINAVNRVF
510
MIETMRAENQ KVAMQ
Length:515
Mass (Da):56,758
Last modified:July 28, 2009 - v1
Checksum:iBD59459DE3FDC9BB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001638 Genomic DNA. Translation: ACS25288.1.
RefSeqiWP_015864699.1. NC_012793.1.
YP_002950554.1. NC_012793.1.

Genome annotation databases

EnsemblBacteriaiACS25288; ACS25288; GWCH70_2593.
KEGGigwc:GWCH70_2593.
PATRICi21974248. VBIGeoSp101709_2726.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001638 Genomic DNA. Translation: ACS25288.1.
RefSeqiWP_015864699.1. NC_012793.1.
YP_002950554.1. NC_012793.1.

3D structure databases

ProteinModelPortaliC5D5M0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi471223.GWCH70_2593.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACS25288; ACS25288; GWCH70_2593.
KEGGigwc:GWCH70_2593.
PATRICi21974248. VBIGeoSp101709_2726.

Phylogenomic databases

eggNOGiCOG0119.
HOGENOMiHOG000046859.
KOiK01649.
OMAiWSVHCHN.
OrthoDBiEOG6CGCF3.

Enzyme and pathway databases

UniPathwayiUPA00048; UER00070.
BioCyciGSP471223:GH2C-2688-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01025. LeuA_type1.
InterProiIPR013709. 2-isopropylmalate_synth_dimer.
IPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR005671. LeuA_bact_synth.
IPR000891. PYR_CT.
[Graphical view]
PfamiPF00682. HMGL-like. 1 hit.
PF08502. LeuA_dimer. 1 hit.
[Graphical view]
SMARTiSM00917. LeuA_dimer. 1 hit.
[Graphical view]
SUPFAMiSSF110921. SSF110921. 1 hit.
TIGRFAMsiTIGR00973. leuA_bact. 1 hit.
PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: WCH70.

Entry informationi

Entry nameiLEU1_GEOSW
AccessioniPrimary (citable) accession number: C5D5M0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: April 1, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.