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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Geobacillus sp. (strain WCH70)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei45 – 451Substrate; via amide nitrogenUniRule annotation
Binding sitei121 – 1211ATPUniRule annotation
Binding sitei167 – 1671ATPUniRule annotation
Binding sitei194 – 1941Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. hydroxyethylthiazole kinase activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. thiamine biosynthetic process Source: UniProtKB-KW
  2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciGSP471223:GH2C-2024-MONOMER.
UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:GWCH70_1939
OrganismiGeobacillus sp. (strain WCH70)
Taxonomic identifieri471223 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
ProteomesiUP000002386 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 269269Hydroxyethylthiazole kinasePRO_1000204360Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi471223.GWCH70_1939.

Structurei

3D structure databases

ProteinModelPortaliC5D2N0.
SMRiC5D2N0. Positions 1-267.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2145.
HOGENOMiHOG000114352.
KOiK00878.
OMAiSPVMAHA.
OrthoDBiEOG628F8M.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00694. thiM. 1 hit.

Sequencei

Sequence statusi: Complete.

C5D2N0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNQEAINAL QMVRQANPLV HNITNVVVTN FTANGLLALG ASPVMAYAKE
60 70 80 90 100
EVADMAKIAG ALVLNIGTLN PTEVEAMIIA GKAANEAGVP VIFDPVGAGA
110 120 130 140 150
TPYRTETARN IASAVQLSVI RGNAAEIANT IGESWTIKGV DAGEGSGDVV
160 170 180 190 200
SLAKKAAKQL KTVVAITGKE DVVTDGNTTY IIRNGHPLLT KVTGTGCLLT
210 220 230 240 250
SVIGAFAAVE RDVLKAAAAA LVIYGVAAEI AAKNAGDEGP GSFQIAFLDA
260
LSRVGADEIS RYGRIEQGE
Length:269
Mass (Da):27,597
Last modified:July 27, 2009 - v1
Checksum:i9A731318936F593D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001638 Genomic DNA. Translation: ACS24672.1.
RefSeqiWP_015864108.1. NC_012793.1.
YP_002949938.1. NC_012793.1.

Genome annotation databases

EnsemblBacteriaiACS24672; ACS24672; GWCH70_1939.
KEGGigwc:GWCH70_1939.
PATRICi21972872. VBIGeoSp101709_2047.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001638 Genomic DNA. Translation: ACS24672.1.
RefSeqiWP_015864108.1. NC_012793.1.
YP_002949938.1. NC_012793.1.

3D structure databases

ProteinModelPortaliC5D2N0.
SMRiC5D2N0. Positions 1-267.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi471223.GWCH70_1939.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACS24672; ACS24672; GWCH70_1939.
KEGGigwc:GWCH70_1939.
PATRICi21972872. VBIGeoSp101709_2047.

Phylogenomic databases

eggNOGiCOG2145.
HOGENOMiHOG000114352.
KOiK00878.
OMAiSPVMAHA.
OrthoDBiEOG628F8M.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.
BioCyciGSP471223:GH2C-2024-MONOMER.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00694. thiM. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: WCH70.

Entry informationi

Entry nameiTHIM_GEOSW
AccessioniPrimary (citable) accession number: C5D2N0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 21, 2009
Last sequence update: July 27, 2009
Last modified: March 31, 2015
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.