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C5D2K6

- MASZ_GEOSW

UniProt

C5D2K6 - MASZ_GEOSW

Protein

Malate synthase G

Gene

glcB

Organism
Geobacillus sp. (strain WCH70)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 37 (01 Oct 2014)
      Sequence version 1 (28 Jul 2009)
      Previous versions | rss
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    • Comment

    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei117 – 1171Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei275 – 2751Acetyl-CoAUniRule annotation
    Binding sitei312 – 3121Acetyl-CoAUniRule annotation
    Active sitei339 – 3391Proton acceptorUniRule annotation
    Binding sitei339 – 3391GlyoxylateUniRule annotation
    Metal bindingi431 – 4311MagnesiumUniRule annotation
    Binding sitei431 – 4311GlyoxylateUniRule annotation
    Metal bindingi459 – 4591MagnesiumUniRule annotation
    Binding sitei540 – 5401Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei630 – 6301Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciGSP471223:GH2C-1998-MONOMER.
    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcBUniRule annotation
    Ordered Locus Names:GWCH70_1913
    OrganismiGeobacillus sp. (strain WCH70)
    Taxonomic identifieri471223 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 727727Malate synthase GPRO_1000212376Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei616 – 6161Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    Oxidation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi471223.GWCH70_1913.

    Structurei

    3D structure databases

    ProteinModelPortaliC5D2K6.
    SMRiC5D2K6. Positions 5-723.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni124 – 1252Acetyl-CoA bindingUniRule annotation
    Regioni456 – 4594Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2225.
    HOGENOMiHOG000220740.
    KOiK01638.
    OMAiSQFIENE.
    OrthoDBiEOG6HJ286.

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.
    ProtoNetiSearch...

    Sequencei

    Sequence statusi: Complete.

    C5D2K6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGEYVKVGNL QVAKLFYEFM NDEVLPGSEV NKEKFWKDFE TLIADLTPKN    50
    KALLARRDEL QAKINEWHKE HRGNFDMEKY KAFLTDIGYL EPEVDDFEIT 100
    TEGVDEEIAV QAGPQLVVPL TNARYALNAA NARWGSLYDA LYGTDAISEE 150
    DGAERGDSYN PVRGAKVIAY AREFLDQAVP LKEYSHKDAV QYAVVDGKLV 200
    VSVEGGSTTT LKEEEKFIGF QGNPENPTAI LLKNNGLHIE IQIDRNHPVG 250
    KTDKAGVKDI YLEAAVTTIM DGEDSVAAVD AEDKVLVYRN LLGLVKGDLT 300
    STFTKGGKTI TRALNPDRVY RTADGKEITL PGRSLMFVRN VGHLMTNNTI 350
    LDANGEEVYE GIMDAVVTSL IMKHSLLGNT RYLNSRKGSF YIVKPKMHGS 400
    EEVAFANELF DRVEDMLGLK RNTIKIGVMD EERRTTLNLK NCIYQVRDRI 450
    IFINTGFLDR TGDEIHTSME AGPMIRKNDM KTSVWLQAYE KSNVTIGLAA 500
    GFQGRAQIGK GMWAMPDMMA EMLKQKGGQL KAGANTAWVP SPTAATLHAL 550
    HYHQVNVAEV QNELRKERKD YRDEILQIPV AVNPQWTPEE IQEELDNNCQ 600
    GILGYVVRWI DQGIGCSKVP DINNIGLMED RATLRISSQH IANWLHHGIC 650
    TKEQVLETLK RMAKVVDEQN AGDPNYRPMA PDYDNSVAFQ AACDLIFKGY 700
    EQPNGYTEPI LHRRRLEAKA KYAAVQQ 727
    Length:727
    Mass (Da):81,560
    Last modified:July 28, 2009 - v1
    Checksum:i388CE77A1DDDCE1C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001638 Genomic DNA. Translation: ACS24648.1.
    RefSeqiWP_015864085.1. NC_012793.1.
    YP_002949914.1. NC_012793.1.

    Genome annotation databases

    EnsemblBacteriaiACS24648; ACS24648; GWCH70_1913.
    GeneIDi7978740.
    KEGGigwc:GWCH70_1913.
    PATRICi21972822. VBIGeoSp101709_2022.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001638 Genomic DNA. Translation: ACS24648.1 .
    RefSeqi WP_015864085.1. NC_012793.1.
    YP_002949914.1. NC_012793.1.

    3D structure databases

    ProteinModelPortali C5D2K6.
    SMRi C5D2K6. Positions 5-723.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 471223.GWCH70_1913.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACS24648 ; ACS24648 ; GWCH70_1913 .
    GeneIDi 7978740.
    KEGGi gwc:GWCH70_1913.
    PATRICi 21972822. VBIGeoSp101709_2022.

    Phylogenomic databases

    eggNOGi COG2225.
    HOGENOMi HOG000220740.
    KOi K01638.
    OMAi SQFIENE.
    OrthoDBi EOG6HJ286.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .
    BioCyci GSP471223:GH2C-1998-MONOMER.

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: WCH70.

    Entry informationi

    Entry nameiMASZ_GEOSW
    AccessioniPrimary (citable) accession number: C5D2K6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: July 28, 2009
    Last modified: October 1, 2014
    This is version 37 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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