Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

C5D2K6

- MASZ_GEOSW

UniProt

C5D2K6 - MASZ_GEOSW

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Malate synthase G

Gene

glcB

Organism
Geobacillus sp. (strain WCH70)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei117 – 1171Acetyl-CoA; via carbonyl oxygenUniRule annotation
Binding sitei275 – 2751Acetyl-CoAUniRule annotation
Binding sitei312 – 3121Acetyl-CoAUniRule annotation
Active sitei339 – 3391Proton acceptorUniRule annotation
Binding sitei339 – 3391GlyoxylateUniRule annotation
Metal bindingi431 – 4311MagnesiumUniRule annotation
Binding sitei431 – 4311GlyoxylateUniRule annotation
Metal bindingi459 – 4591MagnesiumUniRule annotation
Binding sitei540 – 5401Acetyl-CoA; via carbonyl oxygenUniRule annotation
Active sitei630 – 6301Proton donorUniRule annotation

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciGSP471223:GH2C-1998-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:GWCH70_1913
OrganismiGeobacillus sp. (strain WCH70)
Taxonomic identifieri471223 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
ProteomesiUP000002386: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 727727Malate synthase GPRO_1000212376Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei616 – 6161Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi471223.GWCH70_1913.

Structurei

3D structure databases

ProteinModelPortaliC5D2K6.
SMRiC5D2K6. Positions 5-723.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni124 – 1252Acetyl-CoA bindingUniRule annotation
Regioni456 – 4594Glyoxylate bindingUniRule annotation

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiSQFIENE.
OrthoDBiEOG6HJ286.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

C5D2K6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGEYVKVGNL QVAKLFYEFM NDEVLPGSEV NKEKFWKDFE TLIADLTPKN
60 70 80 90 100
KALLARRDEL QAKINEWHKE HRGNFDMEKY KAFLTDIGYL EPEVDDFEIT
110 120 130 140 150
TEGVDEEIAV QAGPQLVVPL TNARYALNAA NARWGSLYDA LYGTDAISEE
160 170 180 190 200
DGAERGDSYN PVRGAKVIAY AREFLDQAVP LKEYSHKDAV QYAVVDGKLV
210 220 230 240 250
VSVEGGSTTT LKEEEKFIGF QGNPENPTAI LLKNNGLHIE IQIDRNHPVG
260 270 280 290 300
KTDKAGVKDI YLEAAVTTIM DGEDSVAAVD AEDKVLVYRN LLGLVKGDLT
310 320 330 340 350
STFTKGGKTI TRALNPDRVY RTADGKEITL PGRSLMFVRN VGHLMTNNTI
360 370 380 390 400
LDANGEEVYE GIMDAVVTSL IMKHSLLGNT RYLNSRKGSF YIVKPKMHGS
410 420 430 440 450
EEVAFANELF DRVEDMLGLK RNTIKIGVMD EERRTTLNLK NCIYQVRDRI
460 470 480 490 500
IFINTGFLDR TGDEIHTSME AGPMIRKNDM KTSVWLQAYE KSNVTIGLAA
510 520 530 540 550
GFQGRAQIGK GMWAMPDMMA EMLKQKGGQL KAGANTAWVP SPTAATLHAL
560 570 580 590 600
HYHQVNVAEV QNELRKERKD YRDEILQIPV AVNPQWTPEE IQEELDNNCQ
610 620 630 640 650
GILGYVVRWI DQGIGCSKVP DINNIGLMED RATLRISSQH IANWLHHGIC
660 670 680 690 700
TKEQVLETLK RMAKVVDEQN AGDPNYRPMA PDYDNSVAFQ AACDLIFKGY
710 720
EQPNGYTEPI LHRRRLEAKA KYAAVQQ
Length:727
Mass (Da):81,560
Last modified:July 28, 2009 - v1
Checksum:i388CE77A1DDDCE1C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001638 Genomic DNA. Translation: ACS24648.1.
RefSeqiWP_015864085.1. NC_012793.1.
YP_002949914.1. NC_012793.1.

Genome annotation databases

EnsemblBacteriaiACS24648; ACS24648; GWCH70_1913.
GeneIDi7978740.
KEGGigwc:GWCH70_1913.
PATRICi21972822. VBIGeoSp101709_2022.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001638 Genomic DNA. Translation: ACS24648.1 .
RefSeqi WP_015864085.1. NC_012793.1.
YP_002949914.1. NC_012793.1.

3D structure databases

ProteinModelPortali C5D2K6.
SMRi C5D2K6. Positions 5-723.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 471223.GWCH70_1913.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACS24648 ; ACS24648 ; GWCH70_1913 .
GeneIDi 7978740.
KEGGi gwc:GWCH70_1913.
PATRICi 21972822. VBIGeoSp101709_2022.

Phylogenomic databases

eggNOGi COG2225.
HOGENOMi HOG000220740.
KOi K01638.
OMAi SQFIENE.
OrthoDBi EOG6HJ286.

Enzyme and pathway databases

UniPathwayi UPA00703 ; UER00720 .
BioCyci GSP471223:GH2C-1998-MONOMER.

Family and domain databases

Gene3Di 2.170.170.11. 2 hits.
HAMAPi MF_00641. Malate_synth_G.
InterProi IPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view ]
Pfami PF01274. Malate_synthase. 1 hit.
[Graphical view ]
SUPFAMi SSF51645. SSF51645. 1 hit.
TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: WCH70.

Entry informationi

Entry nameiMASZ_GEOSW
AccessioniPrimary (citable) accession number: C5D2K6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: November 26, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3