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Protein

Malate synthase G

Gene

glcB

Organism
Geobacillus sp. (strain WCH70)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathway:iglyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase G (glcB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei117 – 1171Acetyl-CoA; via carbonyl oxygenUniRule annotation
Binding sitei275 – 2751Acetyl-CoAUniRule annotation
Binding sitei312 – 3121Acetyl-CoAUniRule annotation
Active sitei339 – 3391Proton acceptorUniRule annotation
Binding sitei339 – 3391GlyoxylateUniRule annotation
Metal bindingi431 – 4311MagnesiumUniRule annotation
Binding sitei431 – 4311GlyoxylateUniRule annotation
Metal bindingi459 – 4591MagnesiumUniRule annotation
Binding sitei540 – 5401Acetyl-CoA; via carbonyl oxygenUniRule annotation
Active sitei630 – 6301Proton donorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciGSP471223:GH2C-1998-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:GWCH70_1913
OrganismiGeobacillus sp. (strain WCH70)
Taxonomic identifieri471223 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
ProteomesiUP000002386 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 727727Malate synthase GPRO_1000212376Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei616 – 6161Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi471223.GWCH70_1913.

Structurei

3D structure databases

ProteinModelPortaliC5D2K6.
SMRiC5D2K6. Positions 5-723.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni124 – 1252Acetyl-CoA bindingUniRule annotation
Regioni456 – 4594Glyoxylate bindingUniRule annotation

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiPKMHGPD.
OrthoDBiEOG6HJ286.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

C5D2K6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGEYVKVGNL QVAKLFYEFM NDEVLPGSEV NKEKFWKDFE TLIADLTPKN
60 70 80 90 100
KALLARRDEL QAKINEWHKE HRGNFDMEKY KAFLTDIGYL EPEVDDFEIT
110 120 130 140 150
TEGVDEEIAV QAGPQLVVPL TNARYALNAA NARWGSLYDA LYGTDAISEE
160 170 180 190 200
DGAERGDSYN PVRGAKVIAY AREFLDQAVP LKEYSHKDAV QYAVVDGKLV
210 220 230 240 250
VSVEGGSTTT LKEEEKFIGF QGNPENPTAI LLKNNGLHIE IQIDRNHPVG
260 270 280 290 300
KTDKAGVKDI YLEAAVTTIM DGEDSVAAVD AEDKVLVYRN LLGLVKGDLT
310 320 330 340 350
STFTKGGKTI TRALNPDRVY RTADGKEITL PGRSLMFVRN VGHLMTNNTI
360 370 380 390 400
LDANGEEVYE GIMDAVVTSL IMKHSLLGNT RYLNSRKGSF YIVKPKMHGS
410 420 430 440 450
EEVAFANELF DRVEDMLGLK RNTIKIGVMD EERRTTLNLK NCIYQVRDRI
460 470 480 490 500
IFINTGFLDR TGDEIHTSME AGPMIRKNDM KTSVWLQAYE KSNVTIGLAA
510 520 530 540 550
GFQGRAQIGK GMWAMPDMMA EMLKQKGGQL KAGANTAWVP SPTAATLHAL
560 570 580 590 600
HYHQVNVAEV QNELRKERKD YRDEILQIPV AVNPQWTPEE IQEELDNNCQ
610 620 630 640 650
GILGYVVRWI DQGIGCSKVP DINNIGLMED RATLRISSQH IANWLHHGIC
660 670 680 690 700
TKEQVLETLK RMAKVVDEQN AGDPNYRPMA PDYDNSVAFQ AACDLIFKGY
710 720
EQPNGYTEPI LHRRRLEAKA KYAAVQQ
Length:727
Mass (Da):81,560
Last modified:July 28, 2009 - v1
Checksum:i388CE77A1DDDCE1C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001638 Genomic DNA. Translation: ACS24648.1.
RefSeqiWP_015864085.1. NC_012793.1.

Genome annotation databases

EnsemblBacteriaiACS24648; ACS24648; GWCH70_1913.
KEGGigwc:GWCH70_1913.
PATRICi21972822. VBIGeoSp101709_2022.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001638 Genomic DNA. Translation: ACS24648.1.
RefSeqiWP_015864085.1. NC_012793.1.

3D structure databases

ProteinModelPortaliC5D2K6.
SMRiC5D2K6. Positions 5-723.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi471223.GWCH70_1913.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACS24648; ACS24648; GWCH70_1913.
KEGGigwc:GWCH70_1913.
PATRICi21972822. VBIGeoSp101709_2022.

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiPKMHGPD.
OrthoDBiEOG6HJ286.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.
BioCyciGSP471223:GH2C-1998-MONOMER.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: WCH70.

Entry informationi

Entry nameiMASZ_GEOSW
AccessioniPrimary (citable) accession number: C5D2K6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: July 22, 2015
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.