Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

C5CXX2 (PROB_VARPS) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate 5-kinase

EC=2.7.2.11
Alternative name(s):
Gamma-glutamyl kinase
Short name=GK
Gene names
Name:proB
Ordered Locus Names:Vapar_4215
OrganismVariovorax paradoxus (strain S110) [Complete proteome] [HAMAP]
Taxonomic identifier543728 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeVariovorax

Protein attributes

Sequence length379 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP-Rule MF_00456

Catalytic activity

ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP-Rule MF_00456

Pathway

Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP-Rule MF_00456

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00456.

Sequence similarities

Belongs to the glutamate 5-kinase family.

Contains 1 PUA domain.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Proline biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processL-proline biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA binding

Inferred from electronic annotation. Source: InterPro

glutamate 5-kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 379379Glutamate 5-kinase HAMAP-Rule MF_00456
PRO_1000206279

Regions

Domain285 – 36379PUA
Nucleotide binding178 – 1792ATP By similarity

Sites

Binding site191ATP By similarity
Binding site591Substrate By similarity
Binding site1461Substrate By similarity
Binding site1581Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
C5CXX2 [UniParc].

Last modified July 28, 2009. Version 1.
Checksum: B6E34B58924BF3D0

FASTA37940,245
        10         20         30         40         50         60 
MTSNSGSTAL RDARRIVVKV GSSLVTNEGR GLDEAAIGEW CRQLAVLVQG GREVVMVSSG 

        70         80         90        100        110        120 
AIAEGMKRLG WRTRPHEVHE LQAAAAVGQM GLAQMYETKL RENRIGSAQV LLTHADLADR 

       130        140        150        160        170        180 
ERYLNARSTL VTLLGLGVVP VINENDTVVN DEIKFGDNDT LGALVANLVE ADALVILTDQ 

       190        200        210        220        230        240 
KGLYTADPRK DPQAKFVHEA AAGDPALEAM AGGAGSSLGR GGMITKILAA KRAAGSGAST 

       250        260        270        280        290        300 
VIAWGREPDA LLRLVRGESI GTLLVAQTAK HQARKRWMAD HLQLRGAVTV DAGAAAKVRA 

       310        320        330        340        350        360 
EGKSLLPIGM TGVSGEFSRG DVIAVRDADG VELARGLANY SSVEARLLCR KPSSEFERLL 

       370 
GYVAEPEMVH RDNMVLMRG 

« Hide

References

[1]"Complete genome sequence of the metabolically versatile plant growth-promoting endophyte, Variovorax paradoxus S110."
Han J.I., Choi H.K., Lee S.W., Orwin P.M., Kim J., Laroe S.L., Kim T.G., O'Neil J., Leadbetter J.R., Lee S.Y., Hur C.G., Spain J.C., Ovchinnikova G., Goodwin L., Han C.
J. Bacteriol. 193:1183-1190(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: S110.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001635 Genomic DNA. Translation: ACS20828.1.
RefSeqYP_002946094.1. NC_012791.1.

3D structure databases

ProteinModelPortalC5CXX2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING543728.Vapar_4215.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACS20828; ACS20828; Vapar_4215.
GeneID7971298.
KEGGvap:Vapar_4215.
PATRIC24008921. VBIVarPar36677_4241.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0263.
HOGENOMHOG000246368.
KOK00931.
OMAMRMIAGH.
OrthoDBEOG6PGK7G.

Enzyme and pathway databases

BioCycVPAR543728:GHLL-4253-MONOMER.
UniPathwayUPA00098; UER00359.

Family and domain databases

Gene3D2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPMF_00456. ProB.
InterProIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFPIRSF000729. GK. 1 hit.
PRINTSPR00474. GLU5KINASE.
SMARTSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsTIGR01027. proB. 1 hit.
PROSITEPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePROB_VARPS
AccessionPrimary (citable) accession number: C5CXX2
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: May 14, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways