C5CSH4 (LEUD_VARPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-isopropylmalate dehydratase small subunit
EC=4.2.1.33

Alternative name(s):
Alpha-IPM isomerase
Short name=IPMI
Isopropylmalate isomerase

Gene names

Name:	leuD
Ordered Locus Names:	Vapar_1421

Organism

Variovorax paradoxus (strain S110) [Complete proteome] [HAMAP]

Taxonomic identifier

543728 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Variovorax › Variovorax paradoxus

Protein attributes

Sequence length	216 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate By similarity. HAMAP-Rule MF_01031
Catalytic activity	(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate. HAMAP-Rule MF_01031
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4. HAMAP-Rule MF_01031
Subunit structure	Heterodimer of LeuC and LeuD By similarity.
Sequence similarities	Belongs to the LeuD family. LeuD type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	leucine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	3-isopropylmalate dehydratase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	3-isopropylmalate dehydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 216	216	3-isopropylmalate dehydratase small subunit HAMAP-Rule MF_01031		PRO_1000213358

Sequences

	Sequence		Length	Mass (Da)
	C5CSH4 [UniParc]. Last modified July 28, 2009. Version 1. Checksum: 8A5635F0B48B0C18	FASTA	216	24,458
	10 20 30 40 50 60 MQKFTVHKGL VAPMDRENVD TDAIIPKQFL KSIKRTGFGQ NLFDEWRYLD AGFPGQDPQS 70 80 90 100 110 120 RKPNPDFVLN QPRYAGASIL LARKNFGCGS SREHAPWALD QYGFRAIIAP SYADIFFNNS 130 140 150 160 170 180 FKNGLLPIVL PEAQVAQLFD ETFAFPGYTL TIDLERQVVV KPDGGEFAFD VQAFRKYCLI 190 200 210 NGLDDIGLTL RHKDKIKAFE AERLAQKPWL AHTMIS

References

[1]

"Complete genome sequence of the metabolically versatile plant growth-promoting endophyte, Variovorax paradoxus S110." Han J.I., Choi H.K., Lee S.W., Orwin P.M., Kim J., Laroe S.L., Kim T.G., O'Neil J., Leadbetter J.R., Lee S.Y., Hur C.G., Spain J.C., Ovchinnikova G., Goodwin L., Han C. J. Bacteriol. 193:1183-1190(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001635 Genomic DNA. Translation: ACS18072.1.
RefSeq	YP_002943338.1. NC_012791.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	543728.Vapar_1421.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACS18072; ACS18072; Vapar_1421.
GeneID	7971249.
KEGG	vap:Vapar_1421.
PATRIC	24003270. VBIVarPar36677_1442.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0066.
HOGENOM	HOG000222939.
KO	K01704.
OMA	YQDNSKR.
ProtClustDB	PRK01641.
Enzyme and pathway databases
UniPathway	UPA00048; UER00071.
Family and domain databases
Gene3D	3.20.19.10. 1 hit.
HAMAP	MF_01031. LeuD_type1.
InterPro	IPR004431. 3-IsopropMal_deHydase_ssu. IPR015937. Acoase/IPM_deHydtase. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR000573. AconitaseA/IPMdHydase_ssu_swvl. [Graphical view]
PANTHER	PTHR11670. PTHR11670. 1 hit. PTHR11670:SF2. PTHR11670:SF2. 1 hit.
Pfam	PF00694. Aconitase_C. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00171. leuD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LEUD_VARPS

Accession

Primary (citable) accession number: C5CSH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	July 28, 2009
Last modified:	April 3, 2013
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program