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C5CNF6 (C5CNF6_VARPS) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:Vapar_0921 EMBL ACS17572.1
OrganismVariovorax paradoxus (strain S110) [Complete proteome] [HAMAP] EMBL ACS17572.1
Taxonomic identifier543728 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeVariovorax

Protein attributes

Sequence length323 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain108 – 312205ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding138 – 19356ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2651Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2791Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2791Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2811Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
C5CNF6 [UniParc].

Last modified July 28, 2009. Version 1.
Checksum: 4157FC4255F962A1

FASTA32334,942
        10         20         30         40         50         60 
MSLQDPKQFG KVAVLFGGSS AEREISLLSG NGVLEALRSR GVDAHAFDPA ERDLVELRRE 

        70         80         90        100        110        120 
GFARCFIALH GRHGEDGTVQ GALELLGIPY TGSGVMASSV AMDKVMTKRI WQADGLPTPR 

       130        140        150        160        170        180 
YVRLAFDQQS REQVRAVPDV LGLPLIVKPP REGSSIGVTK VEGYSQMQDA VTLSARYDAD 

       190        200        210        220        230        240 
VLCEEFIEGE EVTCAVLGQG LDARALPVVR IAAPEGAYDY QNKYFTDDVK YHCPSGLPEA 

       250        260        270        280        290        300 
EEHEIQRITL AAYHTLGCRG WGRADVMIRA SDRKPFLLEM NTSPGMTSHS LVPMSARASG 

       310        320 
IAYEDLCLRV LASASLDAAG GAQ 

« Hide

References

[1]"Complete genome sequence of the metabolically versatile plant growth-promoting endophyte, Variovorax paradoxus S110."
Han J.I., Choi H.K., Lee S.W., Orwin P.M., Kim J., Laroe S.L., Kim T.G., O'Neil J., Leadbetter J.R., Lee S.Y., Hur C.G., Spain J.C., Ovchinnikova G., Goodwin L., Han C.
J. Bacteriol. 193:1183-1190(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: S110.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001635 Genomic DNA. Translation: ACS17572.1.
RefSeqYP_002942838.1. NC_012791.1.

3D structure databases

ProteinModelPortalC5CNF6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING543728.Vapar_0921.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACS17572; ACS17572; Vapar_0921.
GeneID7970096.
KEGGvap:Vapar_0921.
PATRIC24002246. VBIVarPar36677_0932.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMAANDTQYR.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycVPAR543728:GHLL-928-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameC5CNF6_VARPS
AccessionPrimary (citable) accession number: C5CNF6
Entry history
Integrated into UniProtKB/TrEMBL: July 28, 2009
Last sequence update: July 28, 2009
Last modified: June 11, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)