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C5CL84 (PUR9_VARPS) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional purine biosynthesis protein PurH

Including the following 2 domains:

  1. Phosphoribosylaminoimidazolecarboxamide formyltransferase
    EC=2.1.2.3
    Alternative name(s):
    AICAR transformylase
  2. IMP cyclohydrolase
    EC=3.5.4.10
    Alternative name(s):
    ATIC
    IMP synthase
    Inosinicase
Gene names
Name:purH
Ordered Locus Names:Vapar_4630
OrganismVariovorax paradoxus (strain S110) [Complete proteome] [HAMAP]
Taxonomic identifier543728 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeVariovorax

Protein attributes

Sequence length535 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_00139

IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_00139

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. HAMAP-Rule MF_00139

Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.

Domain

The IMP cyclohydrolase activity resides in the N-terminal region By similarity. HAMAP-Rule MF_00139

Sequence similarities

Belongs to the PurH family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 535535Bifunctional purine biosynthesis protein PurH HAMAP-Rule MF_00139
PRO_1000203256

Sequences

Sequence LengthMass (Da)Tools
C5CL84 [UniParc].

Last modified July 28, 2009. Version 1.
Checksum: 0D57D3A5850B0924

FASTA53556,657
        10         20         30         40         50         60 
MAQTALISVS DKTGILEFAQ ALHALGIKLL STGGTAKLLA DAGLPVTEVA DHTGFPEMLD 

        70         80         90        100        110        120 
GRVKTLHPKI HGGLLARRDL PAHVAAIQEH GIDTIDLLVV NLYPFEATVA KAGCTLEDAI 

       130        140        150        160        170        180 
ENIDIGGPAM VRSAAKNWKD VGVLTDASQY AVALAELQAG GKLSDKTKFA FSVAAFNRIA 

       190        200        210        220        230        240 
DYDGAISDYL SAIDFDASIG QASPTRSMFP AQSNGRFVKV QDLRYGENPH QQAAFYRDLH 

       250        260        270        280        290        300 
PAPGSLVSAK QLQGKELSYN NIADADAAWE CVKSFDVPAC VIVKHANPCG VAVGKDAAEA 

       310        320        330        340        350        360 
YGKAFKTDPT SAFGGIIAFN RPVDGETAQA IAKQFVEVLM APGYTPEALA VFQATKVKQN 

       370        380        390        400        410        420 
VRVLEIALPP GGTTDWDNGR NLMDVKRVGS GLLMQTADNH ELAASDLKVV TKKQPTPEQL 

       430        440        450        460        470        480 
QDLLFAWKVA KYVKSNAIVF CAGGMTMGVG AGQMSRLDSA RIASIKAEHA GLSLKGTAVA 

       490        500        510        520        530 
SDAFFPFRDG LDVVVDAGAS CVIQPGGSMR DQEVIDAADE RGVVMVLSGV RHFRH 

« Hide

References

[1]"Complete genome sequence of the metabolically versatile plant growth-promoting endophyte, Variovorax paradoxus S110."
Han J.I., Choi H.K., Lee S.W., Orwin P.M., Kim J., Laroe S.L., Kim T.G., O'Neil J., Leadbetter J.R., Lee S.Y., Hur C.G., Spain J.C., Ovchinnikova G., Goodwin L., Han C.
J. Bacteriol. 193:1183-1190(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: S110.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001635 Genomic DNA. Translation: ACS21235.1.
RefSeqYP_002946501.1. NC_012791.1.

3D structure databases

ProteinModelPortalC5CL84.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING543728.Vapar_4630.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACS21235; ACS21235; Vapar_4630.
GeneID7972840.
KEGGvap:Vapar_4630.
PATRIC24009691. VBIVarPar36677_4620.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0138.
HOGENOMHOG000230373.
KOK00602.
OMARAFKTDP.
OrthoDBEOG6QCDFF.

Enzyme and pathway databases

BioCycVPAR543728:GHLL-4674-MONOMER.
UniPathwayUPA00074; UER00133.
UPA00074; UER00135.

Family and domain databases

Gene3D3.40.140.20. 2 hits.
3.40.50.1380. 1 hit.
HAMAPMF_00139. PurH.
InterProIPR024051. AICAR_Tfase_dom.
IPR002695. AICARFT_IMPCHas.
IPR016193. Cytidine_deaminase-like.
IPR011607. MGS-like_dom.
[Graphical view]
PANTHERPTHR11692. PTHR11692. 1 hit.
PfamPF01808. AICARFT_IMPCHas. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PIRSFPIRSF000414. AICARFT_IMPCHas. 1 hit.
SMARTSM00798. AICARFT_IMPCHas. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMSSF52335. SSF52335. 1 hit.
SSF53927. SSF53927. 1 hit.
TIGRFAMsTIGR00355. purH. 1 hit.
ProtoNetSearch...

Entry information

Entry namePUR9_VARPS
AccessionPrimary (citable) accession number: C5CL84
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: May 14, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways