C5CKT2 (LPXA_VARPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase
Short name=UDP-N-acetylglucosamine acyltransferase
EC=2.3.1.129

Gene names

Name:	lpxA
Ordered Locus Names:	Vapar_2611

Organism

Variovorax paradoxus (strain S110) [Complete proteome] [HAMAP]

Taxonomic identifier

543728 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Variovorax › Variovorax paradoxus

Protein attributes

Sequence length	262 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP-Rule MF_00387
Catalytic activity	(R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] + UDP-N-acetyl-alpha-D-glucosamine = [acyl-carrier-protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine. HAMAP-Rule MF_00387
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 1/6. HAMAP-Rule MF_00387
Subunit structure	Homotrimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxA subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid biosynthesis Lipid metabolism
Cellular component	Cytoplasm
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	lipid A biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 262	262	Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase HAMAP-Rule MF_00387		PRO_1000205801

Sequences

	Sequence		Length	Mass (Da)
	C5CKT2 [UniParc]. Last modified July 28, 2009. Version 1. Checksum: 219680DC22B117AD	FASTA	262	27,880
	10 20 30 40 50 60 MTQVHPTALV DPKAQLDASV SVGPYTVIGP HVRVGAGTTI GAHCVIEGRT TIGRDNRIFQ 70 80 90 100 110 120 FSSLGAIPQD KKYAGEPTEL VIGDRNVIRE FCTFNLGVPG AGGVTTVGND NWIMAYTHIA 130 140 150 160 170 180 HDCHVDNHTT LANNTTLAGH VHLADWVTIG GLTGIHQFVS VGAHAMVGFA SAVSQDVPPF 190 200 210 220 230 240 MLVDGNPLAV RGFNVVGLRR RDFSAPRLAA VKQMHRLLYR QGKTLEEARA GIAALATEMP 250 260 EAAADVALME QFLATSTRGI AR

References

[1]

"Complete genome sequence of the metabolically versatile plant growth-promoting endophyte, Variovorax paradoxus S110." Han J.I., Choi H.K., Lee S.W., Orwin P.M., Kim J., Laroe S.L., Kim T.G., O'Neil J., Leadbetter J.R., Lee S.Y., Hur C.G., Spain J.C., Ovchinnikova G., Goodwin L., Han C. J. Bacteriol. 193:1183-1190(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001635 Genomic DNA. Translation: ACS19236.1.
RefSeq	YP_002944502.1. NC_012791.1.
3D structure databases
ProteinModelPortal	C5CKT2.
ModBase	Search...
Protein-protein interaction databases
STRING	543728.Vapar_2611.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACS19236; ACS19236; Vapar_2611.
GeneID	7972373.
KEGG	vap:Vapar_2611.
PATRIC	24005628. VBIVarPar36677_2605.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1043.
HOGENOM	HOG000294326.
KO	K00677.
OMA	REFCTFN.
Enzyme and pathway databases
UniPathway	UPA00359; UER00477.
Family and domain databases
HAMAP	MF_00387. LpxA.
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR010137. Lipid_A_LpxA. IPR011004. Trimer_LpxA-like. [Graphical view]
Pfam	PF00132. Hexapep. 3 hits. [Graphical view]
PIRSF	PIRSF000456. UDP-GlcNAc_acltr. 1 hit.
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01852. lipid_A_lpxA. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXA_VARPS

Accession

Primary (citable) accession number: C5CKT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	July 28, 2009
Last modified:	April 3, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program