C5CKN8 (COBT_VARPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase
Short name=NN:DBI PRT
EC=2.4.2.21

Alternative name(s):
N(1)-alpha-phosphoribosyltransferase

Gene names

Name:	cobT
Ordered Locus Names:	Vapar_2566

Organism

Variovorax paradoxus (strain S110) [Complete proteome] [HAMAP]

Taxonomic identifier

543728 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Variovorax › Variovorax paradoxus

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of alpha-ribazole-5'-phosphate from nicotinate mononucleotide (NAMN) and 5,6-dimethylbenzimidazole (DMB) By similarity. HAMAP-Rule MF_00230
Catalytic activity	Beta-nicotinate D-ribonucleotide + 5,6-dimethylbenzimidazole = nicotinate + alpha-ribazole 5'-phosphate. HAMAP-Rule MF_00230
Pathway	Nucleoside biosynthesis; alpha-ribazole biosynthesis; alpha-ribazole from 5,6-dimethylbenzimidazole: step 1/2. HAMAP-Rule MF_00230
Sequence similarities	Belongs to the CobT family.

Ontologies

Keywords
Biological process	Cobalamin biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cobalamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW nucleoside biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	cobalamin-transporting ATPase activity Inferred from electronic annotation. Source: HAMAP nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 349	349	Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase HAMAP-Rule MF_00230		PRO_1000204369
Sites
	Active site	315	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	C5CKN8 [UniParc]. Last modified July 28, 2009. Version 1. Checksum: 757EB7F4A05D4140	FASTA	349	35,615
	10 20 30 40 50 60 MTDSNQTIPS ISDIHDGALA ARLQSALDNK TKPLGALGRL ESLALRIGLV LGSETPVLEA 70 80 90 100 110 120 PQMLVCAADH GLAARGVSAF PSDVTWQMVE NFLAGGAAVS VLALQHGLAL TVVDCGVRRD 130 140 150 160 170 180 FQARPGLVSR RIAAGTADAS AGPAMTAEQC AQAIANGREV VRALPGNALL LGEMGIGNSS 190 200 210 220 230 240 AAALLLARLA GLDIDGCTGS GTGLDAAGLA RKREVLRDVL ALHAAASEPL DALAAFGGFE 250 260 270 280 290 300 IATLVGAVLQ AAQERRVIVI DGFIASAAVL VAQALQPHVA QRCVAAHSSA EPGHALLLKH 310 320 330 340 LGLEPLLNLD LRLGEGSGGA LAWPLLESAC RILREMASFE AAGVSRKDS

References

[1]

"Complete genome sequence of the metabolically versatile plant growth-promoting endophyte, Variovorax paradoxus S110." Han J.I., Choi H.K., Lee S.W., Orwin P.M., Kim J., Laroe S.L., Kim T.G., O'Neil J., Leadbetter J.R., Lee S.Y., Hur C.G., Spain J.C., Ovchinnikova G., Goodwin L., Han C. J. Bacteriol. 193:1183-1190(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001635 Genomic DNA. Translation: ACS19192.1.
RefSeq	YP_002944458.1. NC_012791.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	543728.Vapar_2566.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACS19192; ACS19192; Vapar_2566.
GeneID	7970043.
KEGG	vap:Vapar_2566.
PATRIC	24005530. VBIVarPar36677_2558.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2038.
HOGENOM	HOG000263500.
KO	K00768.
OMA	FICSVAA.
ProtClustDB	CLSK951554.
Enzyme and pathway databases
UniPathway	UPA00061; UER00516.
Family and domain databases
Gene3D	1.10.1610.10. 1 hit.
HAMAP	MF_00230. CobT.
InterPro	IPR003200. Nict_dMeBzImd_PRibTrfase-like. IPR023195. Nict_dMeBzImd_PRibTrfase_N. IPR017846. Nict_dMeBzImd_PRibTrfase_pro. [Graphical view]
Pfam	PF02277. DBI_PRT. 1 hit. [Graphical view]
SUPFAM	SSF52733. NN:DBI_PRT. 1 hit.
TIGRFAMs	TIGR03160. cobT_DBIPRT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

COBT_VARPS

Accession

Primary (citable) accession number: C5CKN8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	July 28, 2009
Last modified:	April 3, 2013
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program