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C5CJB5

- MASZ_VARPS

UniProt

C5CJB5 - MASZ_VARPS

Protein

Malate synthase G

Gene

glcB

Organism
Variovorax paradoxus (strain S110)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 36 (01 Oct 2014)
      Sequence version 1 (28 Jul 2009)
      Previous versions | rss
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    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei275 – 2751Acetyl-CoAUniRule annotation
    Binding sitei312 – 3121Acetyl-CoAUniRule annotation
    Active sitei339 – 3391Proton acceptorUniRule annotation
    Binding sitei339 – 3391GlyoxylateUniRule annotation
    Metal bindingi428 – 4281MagnesiumUniRule annotation
    Binding sitei428 – 4281GlyoxylateUniRule annotation
    Metal bindingi456 – 4561MagnesiumUniRule annotation
    Binding sitei537 – 5371Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei632 – 6321Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciVPAR543728:GHLL-453-MONOMER.
    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcBUniRule annotation
    Ordered Locus Names:Vapar_0452
    OrganismiVariovorax paradoxus (strain S110)
    Taxonomic identifieri543728 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeVariovorax
    ProteomesiUP000000453: Chromosome 1

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 724724Malate synthase GPRO_1000212378Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei618 – 6181Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    Oxidation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi543728.Vapar_0452.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni125 – 1262Acetyl-CoA bindingUniRule annotation
    Regioni453 – 4564Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2225.
    HOGENOMiHOG000220740.
    KOiK01638.
    OMAiSQFIENE.
    OrthoDBiEOG6HJ286.

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    C5CJB5-1 [UniParc]FASTAAdd to Basket

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    MTARTTTHGL QVATELHRFI EDKVLPASGV ASDVFWKGFD AIVHDLAPKN    50
    IALLAERDRL QAELDAWHKK NPGPIADMPA YRAFLEKIGY LLPQPKGAKA 100
    TTANVDSELA LQAGPQLVVP ILNARYALNA ANARWGSLYD ALYGTDAIPE 150
    TGGAEKGKGY NPVRGAKVIE FARNVLDQAA PLENGSHKMA TGYSVKDGKL 200
    VVALQSSSTG LADPAQFVGY QGDAAAPSSV LLKHNGIHLD IRIDRSTPIG 250
    KGDAAGVSDL VLEAALSTIL DLEDSVAVVD AADKVVAYSN WLGIVEGTLT 300
    EEVAKGGKTF TRGLNPDREY TGADGRPVKL HGRSLMFLRN VGHLMTNPAV 350
    LYAGGKEIPE GILDAVVTTT IAMIDLKRKG NSRTGSIYIV KPKMHGPAEV 400
    AFASELFGRV EQLLGLPANT VKLGIMDEER RTSVNLKACI AEAAARVAFI 450
    NTGFLDRTGD EMHTAMHGGP MIRKGDMKSS AWIGAYEKNN VLVGLSCGLR 500
    GKAQIGKGMW AMPDLMAAML EQKIAHPKAG ANTAWVPSPT AATLHALHYH 550
    QVSVTAVQQE LEKIDADAER DNILNALLQV PIAPAANWTE AEKQQEIDNN 600
    VQGILGYVVR WIDQGVGCSK VPDIHNVGLM EDRATLRISS QHIANWLLHG 650
    VVTKAQVDET FQRMAKVVDE QNAGDPVYQP MAGHFDTSAA YKAAQDLVFK 700
    GIEQPSGYTE PLLHAWRLKV KGEV 724
    Length:724
    Mass (Da):77,864
    Last modified:July 28, 2009 - v1
    Checksum:i4CE67206D0446EFE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001635 Genomic DNA. Translation: ACS17115.1.
    RefSeqiYP_002942381.1. NC_012791.1.

    Genome annotation databases

    EnsemblBacteriaiACS17115; ACS17115; Vapar_0452.
    GeneIDi7970193.
    KEGGivap:Vapar_0452.
    PATRICi24001300. VBIVarPar36677_0464.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001635 Genomic DNA. Translation: ACS17115.1 .
    RefSeqi YP_002942381.1. NC_012791.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 543728.Vapar_0452.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACS17115 ; ACS17115 ; Vapar_0452 .
    GeneIDi 7970193.
    KEGGi vap:Vapar_0452.
    PATRICi 24001300. VBIVarPar36677_0464.

    Phylogenomic databases

    eggNOGi COG2225.
    HOGENOMi HOG000220740.
    KOi K01638.
    OMAi SQFIENE.
    OrthoDBi EOG6HJ286.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .
    BioCyci VPAR543728:GHLL-453-MONOMER.

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of the metabolically versatile plant growth-promoting endophyte, Variovorax paradoxus S110."
      Han J.I., Choi H.K., Lee S.W., Orwin P.M., Kim J., Laroe S.L., Kim T.G., O'Neil J., Leadbetter J.R., Lee S.Y., Hur C.G., Spain J.C., Ovchinnikova G., Goodwin L., Han C.
      J. Bacteriol. 193:1183-1190(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: S110.

    Entry informationi

    Entry nameiMASZ_VARPS
    AccessioniPrimary (citable) accession number: C5CJB5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: July 28, 2009
    Last modified: October 1, 2014
    This is version 36 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3