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C5CJB5

- MASZ_VARPS

UniProt

C5CJB5 - MASZ_VARPS

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Protein
Malate synthase G
Gene
glcB, Vapar_0452
Organism
Variovorax paradoxus (strain S110)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA By similarity.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygen By similarity
Binding sitei275 – 2751Acetyl-CoA By similarity
Binding sitei312 – 3121Acetyl-CoA By similarity
Active sitei339 – 3391Proton acceptor By similarity
Binding sitei339 – 3391Glyoxylate By similarity
Metal bindingi428 – 4281Magnesium By similarity
Binding sitei428 – 4281Glyoxylate By similarity
Metal bindingi456 – 4561Magnesium By similarity
Binding sitei537 – 5371Acetyl-CoA; via carbonyl oxygen By similarity
Active sitei632 – 6321Proton donor By similarity

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciVPAR543728:GHLL-453-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase G (EC:2.3.3.9)
Gene namesi
Name:glcB
Ordered Locus Names:Vapar_0452
OrganismiVariovorax paradoxus (strain S110)
Taxonomic identifieri543728 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeVariovorax
ProteomesiUP000000453: Chromosome 1

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 724724Malate synthase GUniRule annotation
PRO_1000212378Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei618 – 6181Cysteine sulfenic acid (-SOH) By similarity

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi543728.Vapar_0452.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 1262Acetyl-CoA binding By similarity
Regioni453 – 4564Glyoxylate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiSQFIENE.
OrthoDBiEOG6HJ286.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

C5CJB5-1 [UniParc]FASTAAdd to Basket

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MTARTTTHGL QVATELHRFI EDKVLPASGV ASDVFWKGFD AIVHDLAPKN    50
IALLAERDRL QAELDAWHKK NPGPIADMPA YRAFLEKIGY LLPQPKGAKA 100
TTANVDSELA LQAGPQLVVP ILNARYALNA ANARWGSLYD ALYGTDAIPE 150
TGGAEKGKGY NPVRGAKVIE FARNVLDQAA PLENGSHKMA TGYSVKDGKL 200
VVALQSSSTG LADPAQFVGY QGDAAAPSSV LLKHNGIHLD IRIDRSTPIG 250
KGDAAGVSDL VLEAALSTIL DLEDSVAVVD AADKVVAYSN WLGIVEGTLT 300
EEVAKGGKTF TRGLNPDREY TGADGRPVKL HGRSLMFLRN VGHLMTNPAV 350
LYAGGKEIPE GILDAVVTTT IAMIDLKRKG NSRTGSIYIV KPKMHGPAEV 400
AFASELFGRV EQLLGLPANT VKLGIMDEER RTSVNLKACI AEAAARVAFI 450
NTGFLDRTGD EMHTAMHGGP MIRKGDMKSS AWIGAYEKNN VLVGLSCGLR 500
GKAQIGKGMW AMPDLMAAML EQKIAHPKAG ANTAWVPSPT AATLHALHYH 550
QVSVTAVQQE LEKIDADAER DNILNALLQV PIAPAANWTE AEKQQEIDNN 600
VQGILGYVVR WIDQGVGCSK VPDIHNVGLM EDRATLRISS QHIANWLLHG 650
VVTKAQVDET FQRMAKVVDE QNAGDPVYQP MAGHFDTSAA YKAAQDLVFK 700
GIEQPSGYTE PLLHAWRLKV KGEV 724
Length:724
Mass (Da):77,864
Last modified:July 28, 2009 - v1
Checksum:i4CE67206D0446EFE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001635 Genomic DNA. Translation: ACS17115.1.
RefSeqiYP_002942381.1. NC_012791.1.

Genome annotation databases

EnsemblBacteriaiACS17115; ACS17115; Vapar_0452.
GeneIDi7970193.
KEGGivap:Vapar_0452.
PATRICi24001300. VBIVarPar36677_0464.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001635 Genomic DNA. Translation: ACS17115.1 .
RefSeqi YP_002942381.1. NC_012791.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 543728.Vapar_0452.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACS17115 ; ACS17115 ; Vapar_0452 .
GeneIDi 7970193.
KEGGi vap:Vapar_0452.
PATRICi 24001300. VBIVarPar36677_0464.

Phylogenomic databases

eggNOGi COG2225.
HOGENOMi HOG000220740.
KOi K01638.
OMAi SQFIENE.
OrthoDBi EOG6HJ286.

Enzyme and pathway databases

UniPathwayi UPA00703 ; UER00720 .
BioCyci VPAR543728:GHLL-453-MONOMER.

Family and domain databases

Gene3Di 2.170.170.11. 2 hits.
HAMAPi MF_00641. Malate_synth_G.
InterProi IPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view ]
Pfami PF01274. Malate_synthase. 1 hit.
[Graphical view ]
SUPFAMi SSF51645. SSF51645. 1 hit.
TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the metabolically versatile plant growth-promoting endophyte, Variovorax paradoxus S110."
    Han J.I., Choi H.K., Lee S.W., Orwin P.M., Kim J., Laroe S.L., Kim T.G., O'Neil J., Leadbetter J.R., Lee S.Y., Hur C.G., Spain J.C., Ovchinnikova G., Goodwin L., Han C.
    J. Bacteriol. 193:1183-1190(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: S110.

Entry informationi

Entry nameiMASZ_VARPS
AccessioniPrimary (citable) accession number: C5CJB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: May 14, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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