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C5CJ85 (SYL_VARPS) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Vapar_0422
OrganismVariovorax paradoxus (strain S110) [Complete proteome] [HAMAP]
Taxonomic identifier543728 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeVariovorax

Protein attributes

Sequence length876 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 876876Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000202230

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif634 – 6385"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6371ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
C5CJ85 [UniParc].

Last modified July 28, 2009. Version 1.
Checksum: 7B7703D418F056CA

FASTA87697,816
        10         20         30         40         50         60 
MNPSYKPSDV ESAAQAQWSA ADAYRVTEDA SRKKYYACSM LPYPSGKLHM GHVRNYTIND 

        70         80         90        100        110        120 
MLTRYLRMSG YNVLMPMGWD AFGLPAENAA LKNGVPPAKW TYENIDYMRG QLQAMGLAID 

       130        140        150        160        170        180 
WSREIATCDP SYYKWNQWLF LKMLEKGIAY RKTQVVNWDP VDQTVLANEQ VIDGKGWRTG 

       190        200        210        220        230        240 
ATVERREIPG YYLKISDYAE ELLEHTQHKL PGWPERVKLM QENWIGKSEG VRFAFTHDIQ 

       250        260        270        280        290        300 
DASGRLIQDG RMYVFTTRAD TIMGVTFCAV APEHPLAAHA ATLDPKVAAF IEECKSGGTT 

       310        320        330        340        350        360 
EAELATQEKK GVPTGLTVKH PITEEQVPVW VGNYVLINYG DGAVMGVPAH DERDFAFANK 

       370        380        390        400        410        420 
YGIEIIQVVL VDDEPHFDYH RWQDWYGDKQ RGVTINSDNF SGMTHKEAVA AVAHALAQKG 

       430        440        450        460        470        480 
LGEMQTTWRL RDWGVSRQRY WGTPIPIIHC DEHGAVPVPE KDLPVVLPTD CVPDGSGNPL 

       490        500        510        520        530        540 
AKHEGFHAGV VCPVCGKPAR RETDTMDTFV DSSWYFMRYC DPKNEQAMVA EGADYWMPMD 

       550        560        570        580        590        600 
QYIGGIEHAI LHLLYARFWT KVMRDLGLVK VDEPFSKLLT QGMVLNHIYY HRDEKGGKNY 

       610        620        630        640        650        660 
HPPLEVTPTL DAQGRIVGGT TADGTKIEYG GVGKMGKSER NGVDPQDLIE KYGADTARLY 

       670        680        690        700        710        720 
TMFTAPPEAT LEWNDAAVEG SYRFLRRVWN FGVAQADVAP VAFGGQAFGK AAQALRREVH 

       730        740        750        760        770        780 
TVLRQVDYDY QRMQYNTVVS GAMKLLNALE GFKPDGSAGD AAALREGLGI LLRCLYPATP 

       790        800        810        820        830        840 
HITHQLWQHL GYDKELGDLL DAPWPVVDVA ALEQDEIELM LQVNGKLRGA LRVPAGASKA 

       850        860        870 
EIEALALSCD DFVKFAEGAP AKRVIVVPGR LVNVVI 

« Hide

References

[1]"Complete genome sequence of the metabolically versatile plant growth-promoting endophyte, Variovorax paradoxus S110."
Han J.I., Choi H.K., Lee S.W., Orwin P.M., Kim J., Laroe S.L., Kim T.G., O'Neil J., Leadbetter J.R., Lee S.Y., Hur C.G., Spain J.C., Ovchinnikova G., Goodwin L., Han C.
J. Bacteriol. 193:1183-1190(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: S110.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001635 Genomic DNA. Translation: ACS17085.1.
RefSeqYP_002942351.1. NC_012791.1.

3D structure databases

ProteinModelPortalC5CJ85.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING543728.Vapar_0422.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACS17085; ACS17085; Vapar_0422.
GeneID7970163.
KEGGvap:Vapar_0422.
PATRIC24001238. VBIVarPar36677_0433.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycVPAR543728:GHLL-423-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_VARPS
AccessionPrimary (citable) accession number: C5CJ85
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: May 14, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries