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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Variovorax paradoxus (strain S110)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei637 – 6371ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciVPAR543728:GHLL-423-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Vapar_0422
OrganismiVariovorax paradoxus (strain S110)
Taxonomic identifieri543728 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeVariovorax
ProteomesiUP000000453 Componenti: Chromosome 1

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 876876Leucine--tRNA ligasePRO_1000202230Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi543728.Vapar_0422.

Structurei

3D structure databases

ProteinModelPortaliC5CJ85.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi634 – 6385"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C5CJ85-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPSYKPSDV ESAAQAQWSA ADAYRVTEDA SRKKYYACSM LPYPSGKLHM
60 70 80 90 100
GHVRNYTIND MLTRYLRMSG YNVLMPMGWD AFGLPAENAA LKNGVPPAKW
110 120 130 140 150
TYENIDYMRG QLQAMGLAID WSREIATCDP SYYKWNQWLF LKMLEKGIAY
160 170 180 190 200
RKTQVVNWDP VDQTVLANEQ VIDGKGWRTG ATVERREIPG YYLKISDYAE
210 220 230 240 250
ELLEHTQHKL PGWPERVKLM QENWIGKSEG VRFAFTHDIQ DASGRLIQDG
260 270 280 290 300
RMYVFTTRAD TIMGVTFCAV APEHPLAAHA ATLDPKVAAF IEECKSGGTT
310 320 330 340 350
EAELATQEKK GVPTGLTVKH PITEEQVPVW VGNYVLINYG DGAVMGVPAH
360 370 380 390 400
DERDFAFANK YGIEIIQVVL VDDEPHFDYH RWQDWYGDKQ RGVTINSDNF
410 420 430 440 450
SGMTHKEAVA AVAHALAQKG LGEMQTTWRL RDWGVSRQRY WGTPIPIIHC
460 470 480 490 500
DEHGAVPVPE KDLPVVLPTD CVPDGSGNPL AKHEGFHAGV VCPVCGKPAR
510 520 530 540 550
RETDTMDTFV DSSWYFMRYC DPKNEQAMVA EGADYWMPMD QYIGGIEHAI
560 570 580 590 600
LHLLYARFWT KVMRDLGLVK VDEPFSKLLT QGMVLNHIYY HRDEKGGKNY
610 620 630 640 650
HPPLEVTPTL DAQGRIVGGT TADGTKIEYG GVGKMGKSER NGVDPQDLIE
660 670 680 690 700
KYGADTARLY TMFTAPPEAT LEWNDAAVEG SYRFLRRVWN FGVAQADVAP
710 720 730 740 750
VAFGGQAFGK AAQALRREVH TVLRQVDYDY QRMQYNTVVS GAMKLLNALE
760 770 780 790 800
GFKPDGSAGD AAALREGLGI LLRCLYPATP HITHQLWQHL GYDKELGDLL
810 820 830 840 850
DAPWPVVDVA ALEQDEIELM LQVNGKLRGA LRVPAGASKA EIEALALSCD
860 870
DFVKFAEGAP AKRVIVVPGR LVNVVI
Length:876
Mass (Da):97,816
Last modified:July 28, 2009 - v1
Checksum:i7B7703D418F056CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001635 Genomic DNA. Translation: ACS17085.1.
RefSeqiYP_002942351.1. NC_012791.1.

Genome annotation databases

EnsemblBacteriaiACS17085; ACS17085; Vapar_0422.
KEGGivap:Vapar_0422.
PATRICi24001238. VBIVarPar36677_0433.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001635 Genomic DNA. Translation: ACS17085.1.
RefSeqiYP_002942351.1. NC_012791.1.

3D structure databases

ProteinModelPortaliC5CJ85.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi543728.Vapar_0422.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACS17085; ACS17085; Vapar_0422.
KEGGivap:Vapar_0422.
PATRICi24001238. VBIVarPar36677_0433.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciVPAR543728:GHLL-423-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the metabolically versatile plant growth-promoting endophyte, Variovorax paradoxus S110."
    Han J.I., Choi H.K., Lee S.W., Orwin P.M., Kim J., Laroe S.L., Kim T.G., O'Neil J., Leadbetter J.R., Lee S.Y., Hur C.G., Spain J.C., Ovchinnikova G., Goodwin L., Han C.
    J. Bacteriol. 193:1183-1190(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: S110.

Entry informationi

Entry nameiSYL_VARPS
AccessioniPrimary (citable) accession number: C5CJ85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: April 1, 2015
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.