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C5CHA1

- SYI_KOSOT

UniProt

C5CHA1 - SYI_KOSOT

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Kosmotoga olearia (strain TBF 19.5.1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 37 (01 Oct 2014)
      Sequence version 1 (28 Jul 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei551 – 5511Aminoacyl-adenylateUniRule annotation
    Binding sitei595 – 5951ATPUniRule annotation
    Metal bindingi885 – 8851ZincUniRule annotation
    Metal bindingi888 – 8881ZincUniRule annotation
    Metal bindingi905 – 9051ZincUniRule annotation
    Metal bindingi908 – 9081ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciKOLE521045:GHRV-11-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Kole_0011
    OrganismiKosmotoga olearia (strain TBF 19.5.1)
    Taxonomic identifieri521045 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeKosmotoga
    ProteomesiUP000002382: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 921921Isoleucine--tRNA ligasePRO_1000216238Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi521045.Kole_0011.

    Structurei

    3D structure databases

    ProteinModelPortaliC5CHA1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi592 – 5965"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    C5CHA1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYKDTLNLP KTSFSMRANL VRKEPEQLKK WEEMDLYKYV QKNREGAPLF    50
    VLHDGPPYAN GNIHVGTALN KILKDFIIKY KTMQGYNAPY IPGWDTHGLP 100
    IEHRVASELG EKAKELSKST IRKKCRKYAE KYVKIQREQF KRLGVRGDWE 150
    NPYLTFDPKY EASVLKVLSK LVKSGNVYRT LKPIYWCTSC ETALAEAEVE 200
    YHDHKSYSIY VKFQDKNKPN RFFVIWTTTP WTLPANVAIA LNPNFDYCEV 250
    KVKDEIWILA EGLLEQAMKA MGIESYEIIS TKKGSEFEGE VAQHPFMNRD 300
    SVIVMADYVA LDAGTGCVHT APGHGEEDYG TGLRYRLPVI SPVNDQGIFT 350
    KEAGKYAGLF VFDANPIIVE DLRKSGHLVG EGKIEHSYPH CWRCKNPVIF 400
    RATEQWFIDV DKNDLRQKAL SEIEKTEWHP SWGQNRITAM VQDRPDWCIS 450
    RQRSWGIPIP AFRCQDCGES ILDGKVIDHF AGIVEEKGTD VWFEWSESDL 500
    LPEGYTCPKC GSKNLKKQED ILDVWVDSGS SFEAVVKVMD ELRFPADVYI 550
    EGSDQHRGWF QSSLLLSVGT EGVAPFKSVI THGFINDEQG RKMSKSLGNA 600
    VDPQEINNKY GAEILRLWVA SSNYQEDIRI SMNIIQQQIE NYKKIRNTLR 650
    YLLGNLSDYS PQDSVPYEEL LELDKWAIMK LHKLIRDVTR YYDRYEFYKV 700
    FQSILKFIIT DMSAFYLDII KDRLYVEGKT SPERRSAQTV LFEILVALNK 750
    MLAPILTFTS EEVYAHLPEA ARNYKTIQVE NWPEYKEEYI DSSLEERWEK 800
    LINLREDVLK ALEIARAEKL IGNSLDAKVT LQIRSKDIDE IVRQYSREFI 850
    ADLFIVSQVE FAESVEGFNG EYAVVKVDRA EGEKCQRCWK YSIETGADPE 900
    LPDMCPRCVA VLRKYGSDYE L 921
    Length:921
    Mass (Da):106,289
    Last modified:July 28, 2009 - v1
    Checksum:i541E12C0770C7733
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001634 Genomic DNA. Translation: ACR78740.1.
    RefSeqiYP_002939744.1. NC_012785.1.

    Genome annotation databases

    EnsemblBacteriaiACR78740; ACR78740; Kole_0011.
    GeneIDi7968836.
    KEGGikol:Kole_0011.
    PATRICi22185108. VBIKosOle109242_0011.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001634 Genomic DNA. Translation: ACR78740.1 .
    RefSeqi YP_002939744.1. NC_012785.1.

    3D structure databases

    ProteinModelPortali C5CHA1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 521045.Kole_0011.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACR78740 ; ACR78740 ; Kole_0011 .
    GeneIDi 7968836.
    KEGGi kol:Kole_0011.
    PATRICi 22185108. VBIKosOle109242_0011.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci KOLE521045:GHRV-11-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of Thermotogales bacterium TBF 19.5.1."
      US DOE Joint Genome Institute
      Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Noll K.
      Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: TBF 19.5.1.

    Entry informationi

    Entry nameiSYI_KOSOT
    AccessioniPrimary (citable) accession number: C5CHA1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: July 28, 2009
    Last modified: October 1, 2014
    This is version 37 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3