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C5CEF0 (C5CEF0_KOSOT) Unreviewed, UniProtKB/TrEMBL

Last modified May 29, 2013. Version 31. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Tyrosine--tRNA ligase HAMAP-Rule MF_02007
EC=6.1.1.1 HAMAP-Rule MF_02007
Alternative name(s):
Tyrosyl-tRNA synthetase HAMAP-Rule MF_02007
Gene names
Name:tyrS HAMAP-Rule MF_02007
Ordered Locus Names:Kole_1499 EMBL ACR80190.1
OrganismKosmotoga olearia (strain TBF 19.5.1) [Complete proteome] [HAMAP] EMBL ACR80190.1
Taxonomic identifier521045 [NCBI]
Taxonomic lineageBacteria › Thermotogae › Thermotogales › Thermotogaceae › Kosmotoga ›

Protein attributes

Sequence length397 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity. HAMAP-Rule MF_02007 SAAS SAAS002307

Catalytic activity

ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP-Rule MF_02007 SAAS SAAS002307

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_02007 SAAS SAAS002307

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02007.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 2 subfamily. HAMAP-Rule MF_02007

Contains 1 S4 RNA-binding domain. HAMAP-Rule MF_02007 SAAS SAAS002307

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain336 – 39762S4 RNA-binding By similarity HAMAP-Rule MF_02007
Motif42 – 5110"HIGH" region By similarity HAMAP-Rule MF_02007
Motif226 – 2305"KMSKS" region By similarity HAMAP-Rule MF_02007

Sites

Binding site2291ATP By similarity HAMAP-Rule MF_02007

Sequences

Sequence LengthMass (Da)Tools
C5CEF0 [UniParc].

Last modified July 28, 2009. Version 1.
Checksum: 861685EA060008F4

FASTA39745,748
        10         20         30         40         50         60 
MEVPEKQLQI LKRNSVSLIS EEELIERLKL GRPLRVKLGV DPSRPDLHLG HAVVLRKLRE 

        70         80         90        100        110        120 
FQDLGHHVIL IIGDFTARIG DPSGRSKTRP MLSKEEAKQN AITYSQQAFK ILDRDKTEIK 

       130        140        150        160        170        180 
FNSEWLDGMS FEDVIRLSAK YTVARMLERN DFEKRYKNNE PISIAEFLYP LAQAYDSVVV 

       190        200        210        220        230        240 
KADVEIGGDD QFFNFVVARK IQEEYGLQPQ VILTMPLIEG TDGNLKMSKS YGNYVAFEDP 

       250        260        270        280        290        300 
PEEMFGKLMS IPDTLIIKYM RLLTDIPEER ILQYERDIKS RKINPRDVKM LLAFEITRFF 

       310        320        330        340        350        360 
HGEEAAVRAR ENFIKVFRKK ELPSEIPEIK VKESELSLVD LMVKYANISS KSEARRLISQ 

       370        380        390 
GGVRLNNTVI KDIHAKVAVS NGDILRIGKR RFFKISC

« Hide

References

[1]"Complete sequence of Thermotogales bacterium TBF 19.5.1."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Noll K.
Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: TBF 19.5.1 EMBL ACR80190.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001634 Genomic DNA. Translation: ACR80190.1.
RefSeqYP_002941194.1. NC_012785.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRING521045.Kole_1499.

Proteomic databases

PRIDEC5CEF0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACR80190; ACR80190; Kole_1499.
GeneID7969143.
KEGGkol:Kole_1499.
PATRIC22188306. VBIKosOle109242_1577.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0162.
HOGENOMHOG000242791.
KOK01866.
ProtClustDBPRK05912.

Enzyme and pathway databases

BioCycKOLE521045:GHRV-1535-MONOMER.

Family and domain databases

Gene3D3.10.290.10. 1 hit.
3.40.50.620. 1 hit.
HAMAPMF_02007. Tyr_tRNA_synth_type2.
InterProIPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002942. S4_RNA-bd.
IPR002307. Tyr-tRNA-ligase.
IPR024088. Tyr-tRNA-ligase_bac-type.
IPR024108. Tyr-tRNA-ligase_bac_2.
[Graphical view]
PANTHERPTHR11766. PTHR11766. 1 hit.
PfamPF01479. S4. 1 hit.
PF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSPR01040. TRNASYNTHTYR.
SMARTSM00363. S4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00234. tyrS. 1 hit.
PROSITEPS50889. S4. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameC5CEF0_KOSOT
AccessionPrimary (citable) accession number: C5CEF0
Entry history
Integrated into UniProtKB/TrEMBL: July 28, 2009
Last sequence update: July 28, 2009
Last modified: May 29, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info