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Protein

Glutamate 5-kinase

Gene

proB

Organism
Kosmotoga olearia (strain TBF 19.5.1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathway:iL-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei7 – 71ATPUniRule annotation
Binding sitei47 – 471SubstrateUniRule annotation
Binding sitei135 – 1351SubstrateUniRule annotation
Binding sitei147 – 1471Substrate; via amide nitrogenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi202 – 2087ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciKOLE521045:GHRV-1452-MONOMER.
UniPathwayiUPA00098; UER00359.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:Kole_1419
OrganismiKosmotoga olearia (strain TBF 19.5.1)
Taxonomic identifieri521045 [NCBI]
Taxonomic lineageiBacteriaThermotogaeKosmotogalesKosmotogaceaeKosmotoga
ProteomesiUP000002382 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 359359Glutamate 5-kinasePRO_1000206272Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi521045.Kole_1419.

Structurei

3D structure databases

ProteinModelPortaliC5CE10.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini266 – 34378PUAUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation
Contains 1 PUA domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0263.
HOGENOMiHOG000246369.
KOiK00931.
OMAiMNKQWIA.
OrthoDBiEOG6PGK7G.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C5CE10-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKITIKVGS NLLVQQDGQL DKRYIVELCR EIGNLMSEGH QVVLVSSGAR
60 70 80 90 100
AAGYGYLNKS NAQEADLYMK QALCAVGQVQ LMKLYESAMS FYGIKVAQIL
110 120 130 140 150
LTREDFSHRK RFLNLRNTLI GLTEMGILPI VNENDSVATE EIMFGDNDVL
160 170 180 190 200
ASMFAIGWNA DYLLLMTSVD GVIDQNGKVI PFYREDTTNM AIAKNASSRW
210 220 230 240 250
GSGGITSKIR AARAAAAAGI QTSICNGKKL ENIAQFVLTG QTGTVFERVG
260 270 280 290 300
PIKAKKAWIG FLSKPKGSIF INEGAKIAIE NNRSLLPVGV VHIEGDFQAG
310 320 330 340 350
DVVEIRLDDG TFLGKGIINF SSAEAKKIVG LKSDQLEDVL GYSCSKVLIH

IDNLWKRDN
Length:359
Mass (Da):39,174
Last modified:July 28, 2009 - v1
Checksum:i43137309B2A700A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001634 Genomic DNA. Translation: ACR80112.1.
RefSeqiWP_015868759.1. NC_012785.1.

Genome annotation databases

EnsemblBacteriaiACR80112; ACR80112; Kole_1419.
KEGGikol:Kole_1419.
PATRICi22188130. VBIKosOle109242_1492.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001634 Genomic DNA. Translation: ACR80112.1.
RefSeqiWP_015868759.1. NC_012785.1.

3D structure databases

ProteinModelPortaliC5CE10.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi521045.Kole_1419.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACR80112; ACR80112; Kole_1419.
KEGGikol:Kole_1419.
PATRICi22188130. VBIKosOle109242_1492.

Phylogenomic databases

eggNOGiCOG0263.
HOGENOMiHOG000246369.
KOiK00931.
OMAiMNKQWIA.
OrthoDBiEOG6PGK7G.

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.
BioCyciKOLE521045:GHRV-1452-MONOMER.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of Thermotogales bacterium TBF 19.5.1."
    US DOE Joint Genome Institute
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Noll K.
    Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TBF 19.5.1.

Entry informationi

Entry nameiPROB_KOSOT
AccessioniPrimary (citable) accession number: C5CE10
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: July 22, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.