C5CC66 (EFTU_MICLC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor Tu
Short name=EF-Tu

Gene names

Name:	tuf
Ordered Locus Names:	Mlut_17200

Organism

Micrococcus luteus (strain ATCC 4698 / DSM 20030 / JCM 1464 / NBRC 3333 / NCIMB 9278 / NCTC 2665 / VKM Ac-2230) (Micrococcus lysodeikticus) [Complete proteome] [HAMAP]

Taxonomic identifier

465515 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Micrococcus › Micrococcus luteus

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis By similarity. HAMAP-Rule MF_00118
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: HAMAP GTPase activity Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	Elongation factor Tu HAMAP-Rule MF_00118		PRO_1000203016
Regions
	Nucleotide binding	19 – 26	8	GTP By similarity
	Nucleotide binding	83 – 87	5	GTP By similarity
	Nucleotide binding	138 – 141	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	C5CC66 [UniParc]. Last modified July 28, 2009. Version 1. Checksum: D65F3695EBE20F73	FASTA	396	43,802
	10 20 30 40 50 60 MAKAKFERTK PHVNIGTIGH VDHGKTTLTA AISKVLYDKY PDLNEARDFA TIDSAPEERQ 70 80 90 100 110 120 RGITINISHV EYQTEKRHYA HVDAPGHADY IKNMITGAAQ MDGAILVVAA TDGPMAQTRE 130 140 150 160 170 180 HVLLARQVGV PALLVALNKS DMVEDEELLE LVEMEVRELL SSQEFDGDEA PVIRTSGLKA 190 200 210 220 230 240 LEGDPQWVKS VEDLMDAVDE YIPDPVRDKD KPFLMPIEDV FTITGRGTVV TGRAERGTLK 250 260 270 280 290 300 INSEVEIVGI RDVQKTTVTG IEMFHKQLDE AWAGENCGLL LRGLKRDDVE RGQVVVEPGS 310 320 330 340 350 360 ITPHTNFEAN VYILSKDEGG RHNPFYSNYR PQFYFRTTDV TGVITLPEGT EMVMPGDTTE 370 380 390 MSVELIQPIA MEEGLGFAIR EGGRTVGSGR VTKITK

References

[1]

"Genome sequence of the Fleming strain of Micrococcus luteus, a simple free-living actinobacterium." Young M., Artsatbanov V., Beller H.R., Chandra G., Chater K.F., Dover L.G., Goh E.B., Kahan T., Kaprelyants A.S., Kyrpides N., Lapidus A., Lowry S.R., Lykidis A., Mahillon J., Markowitz V., Mavromatis K., Mukamolova G.V., Oren A. , Rokem J.S., Smith M.C., Young D.I., Greenblatt C.L. J. Bacteriol. 192:841-860(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 4698 / DSM 20030 / JCM 1464 / NBRC 3333 / NCIMB 9278 / NCTC 2665 / VKM Ac-2230.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001628 Genomic DNA. Translation: ACS31207.1.
RefSeq	YP_002957761.1. NC_012803.1.
3D structure databases
ProteinModelPortal	C5CC66.
SMR	C5CC66. Positions 2-394.
ModBase	Search...
Protein-protein interaction databases
STRING	465515.Mlut_17200.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACS31207; ACS31207; Mlut_17200.
GeneID	7985012.
KEGG	mlu:Mlut_17200.
PATRIC	22624080. VBIMicLut29563_1659.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0050.
HOGENOM	HOG000229290.
KO	K02358.
ProtClustDB	PRK00049.
Enzyme and pathway databases
BioCyc	MLUT465515:GHH6-1717-MONOMER.
Family and domain databases
HAMAP	MF_00118_B. EF_Tu_B.
InterPro	IPR000795. EF_GTP-bd_dom. IPR005225. Small_GTP-bd_dom. IPR009001. Transl_elong_EF1A/Init_IF2_C. IPR004161. Transl_elong_EFTu/EF1A_2. IPR004541. Transl_elong_EFTu/EF1A_bac/org. IPR004160. Transl_elong_EFTu/EF1A_C. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Pfam	PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF03143. GTP_EFTU_D3. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SUPFAM	SSF50465. Elong_init_C. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00485. EF-Tu. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFTU_MICLC

Accession

Primary (citable) accession number: C5CC66

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	July 28, 2009
Last modified:	May 1, 2013
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program