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C5CAR9 (C5CAR9_MICLC) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
3-oxoacyl-[acyl-carrier-protein] synthase 3 HAMAP-Rule MF_01815

EC=2.3.1.180 HAMAP-Rule MF_01815
Alternative name(s):
3-oxoacyl-[acyl-carrier-protein] synthase III HAMAP-Rule MF_01815
Beta-ketoacyl-ACP synthase III HAMAP-Rule MF_01815
Gene names
Name:fabH HAMAP-Rule MF_01815
Ordered Locus Names:Mlut_09310 EMBL ACS30452.1
OrganismMicrococcus luteus (strain ATCC 4698 / DSM 20030 / JCM 1464 / NBRC 3333 / NCIMB 9278 / NCTC 2665 / VKM Ac-2230) (Micrococcus lysodeikticus) [Complete proteome] [HAMAP] EMBL ACS30452.1
Taxonomic identifier465515 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeMicrococcus

Protein attributes

Sequence length350 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched-chain and/or straight-chain of fatty acids By similarity. HAMAP-Rule MF_01815

Catalytic activity

Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacetyl-[acyl-carrier-protein] + CoA + CO2. HAMAP-Rule MF_01815 SAAS SAAS004655

Pathway

Lipid metabolism; fatty acid biosynthesis. HAMAP-Rule MF_01815 SAAS SAAS004655

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01815 SAAS SAAS004655

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01815 SAAS SAAS004655.

Domain

The last Arg residue of the ACP-binding site is essential for the weak association between ACP/AcpP and FabH By similarity. HAMAP-Rule MF_01815

Sequence similarities

Belongs to the FabH family. HAMAP-Rule MF_01815

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Region276 – 2805ACP-binding By similarity HAMAP-Rule MF_01815

Sites

Active site1231 By similarity HAMAP-Rule MF_01815
Active site2751 By similarity HAMAP-Rule MF_01815
Active site3061 By similarity HAMAP-Rule MF_01815

Sequences

Sequence LengthMass (Da)Tools
C5CAR9 [UniParc].

Last modified July 28, 2009. Version 1.
Checksum: 7CD8432B19C723B7

FASTA35036,749
        10         20         30         40         50         60 
MTVTLKQHER PAASRIVAVG AYRPANLVPN EDLIGPIDSS DEWIRQRTGI VTRQRATAEE 

        70         80         90        100        110        120 
TVPVMAVGAA REALERAGLQ GSDLDAVIVS TVTFPHATPS AAALVAHEIG ATPAPAYDVS 

       130        140        150        160        170        180 
AACAGYCYGV AQADALVRSG TARHVLVVGV ERLSDVVDPT DRSISFLLGD GAGAVIVAAS 

       190        200        210        220        230        240 
DEPGISPSVW GSDGERWSTI SMTHSQLELR DAVEHARTTG DASAITGAEG MLWPTLRQDG 

       250        260        270        280        290        300 
PSVFRWAVWS MAKVAREALD AAGVEPEDLA AFIPHQANMR IIDEFAKQLK LPESVVVARD 

       310        320        330        340        350 
IADAGNTSAA SIPLAMHRLL EENPELSGGL ALQIGFGAGL VYGAQVVRLP 

« Hide

References

« Hide 'large scale' references
[1]"Genome sequence of the Fleming strain of Micrococcus luteus, a simple free-living actinobacterium."
Young M., Artsatbanov V., Beller H.R., Chandra G., Chater K.F., Dover L.G., Goh E.B., Kahan T., Kaprelyants A.S., Kyrpides N., Lapidus A., Lowry S.R., Lykidis A., Mahillon J., Markowitz V., Mavromatis K., Mukamolova G.V., Oren A. expand/collapse author list , Rokem J.S., Smith M.C., Young D.I., Greenblatt C.L.
J. Bacteriol. 192:841-860(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 4698 / DSM 20030 / JCM 1464 / NBRC 3333 / NCIMB 9278 / NCTC 2665 / VKM Ac-2230.
[2]"Structure of FabH and factors affecting the distribution of branched fatty acids in Micrococcus luteus."
Pereira J.H., Goh E.B., Keasling J.D., Beller H.R., Adams P.D.
Acta Crystallogr. D 68:1320-1328(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001628 Genomic DNA. Translation: ACS30452.1.
RefSeqYP_002957006.1. NC_012803.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4EWPX-ray2.20A/B/C/D/E/F1-350[»]
ProteinModelPortalC5CAR9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING465515.Mlut_09310.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACS30452; ACS30452; Mlut_09310.
GeneID7986167.
KEGGmlu:Mlut_09310.
PATRIC22622508. VBIMicLut29563_0892.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0332.
HOGENOMHOG000246674.
KOK00648.
OrthoDBEOG6J74XN.

Enzyme and pathway databases

BioCycMLUT465515:GHH6-929-MONOMER.
UniPathwayUPA00094.

Family and domain databases

Gene3D3.40.47.10. 2 hits.
HAMAPMF_01815. FabH.
InterProIPR013751. ACP_syn_III.
IPR013747. ACP_syn_III_C.
IPR004655. FabH_synth.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamPF08545. ACP_syn_III. 1 hit.
PF08541. ACP_syn_III_C. 1 hit.
[Graphical view]
SUPFAMSSF53901. SSF53901. 1 hit.
TIGRFAMsTIGR00747. fabH. 1 hit.
ProtoNetSearch...

Entry information

Entry nameC5CAR9_MICLC
AccessionPrimary (citable) accession number: C5CAR9
Entry history
Integrated into UniProtKB/TrEMBL: July 28, 2009
Last sequence update: July 28, 2009
Last modified: June 11, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)