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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Micrococcus luteus (strain ATCC 4698 / DSM 20030 / JCM 1464 / NBRC 3333 / NCIMB 9278 / NCTC 2665 / VKM Ac-2230) (Micrococcus lysodeikticus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi523 – 5231MagnesiumUniRule annotation
Metal bindingi529 – 5291MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciMLUT465515:GHH6-709-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Mlut_07100
OrganismiMicrococcus luteus (strain ATCC 4698 / DSM 20030 / JCM 1464 / NBRC 3333 / NCIMB 9278 / NCTC 2665 / VKM Ac-2230) (Micrococcus lysodeikticus)
Taxonomic identifieri465515 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesMicrococcaceaeMicrococcus
ProteomesiUP000000738 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 753753Polyribonucleotide nucleotidyltransferasePRO_1000215664Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi465515.MlutN2_010100003623.

Structurei

3D structure databases

ProteinModelPortaliC5C9U0.
SMRiC5C9U0. Positions 5-726.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini589 – 64860KHUniRule annotationAdd
BLAST
Domaini660 – 73273S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR014069. pppGpp_PNP.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
TIGR02696. pppGpp_PNP. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C5C9U0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGPEITFAE AVIDNGSYGK RTVRFETGRL AQQAAGAAMV YLDEETSMLS
60 70 80 90 100
ATTVGKSPRE GFDFFPLTVD VEERMYASGR IPGSFFRREG RPSTDAILTC
110 120 130 140 150
RLIDRPLRPA FVKGIRNEVQ VVVTVTSIAP DEIYDTVAIN AASLSTQLSG
160 170 180 190 200
LPFSGPIGGV RMALMDDGSG ARQWVAFPKH SQLKGAVFNM AVAGRVVGDD
210 220 230 240 250
VAIMMVEAEA TPDSWTLVKE RGAQAPTEEI VAEGLEAAKP FIRALCEAQA
260 270 280 290 300
DLVKRAGKDP VDVPVFQDYQ DDAYAAVEKL ATDRLTEIFS IADKQERESA
310 320 330 340 350
SYAYLLEVLE ELAGEGKDFA ERKGEIAKAY GSLTKQIVRR RILTDQVRID
360 370 380 390 400
GRGLTDIRKL TAEVEVLPRV HGSAIFERGE TQIMGVTTLN MLKMEQQIDS
410 420 430 440 450
LSPETAKRYM HHYNFPPYST GETGRVGSPK RREIGHGALA ERALVPVLPA
460 470 480 490 500
REEFPYAIRQ VSEALSSNGS TSMGSVCAST LSLLNAGVPL RAHVAGIAMG
510 520 530 540 550
LVSAEVDGQT QYAALTDILG AEDAFGDMDF KVAGTAEFVT AIQLDTKLDG
560 570 580 590 600
IPASVLASAL TQAREARLHI LSVLNAAIDA PDEMAPTAPR IISVRIPVDK
610 620 630 640 650
IGAVIGPKGA MINQIQDDTG ADITIEDDGT VLIGATDGAS AEAARSAVNA
660 670 680 690 700
IANPQVPEVG ERYLGTVVKL TTFGAFVSLT PGKDGLLHVS ELRKLNEGKR
710 720 730 740 750
VDDVEDVLGV GQKVQVEITK IDDRGKLSLS PVGAESDAVA ETADAIESSQ

TEA
Length:753
Mass (Da):80,493
Last modified:July 28, 2009 - v1
Checksum:iCEB3A92448CFD330
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001628 Genomic DNA. Translation: ACS30242.1.
RefSeqiWP_010079121.1. NZ_CABC01000046.1.
YP_002956796.1. NC_012803.1.

Genome annotation databases

EnsemblBacteriaiACS30242; ACS30242; Mlut_07100.
GeneIDi23700070.
KEGGimlu:Mlut_07100.
PATRICi22622057. VBIMicLut29563_0673.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001628 Genomic DNA. Translation: ACS30242.1.
RefSeqiWP_010079121.1. NZ_CABC01000046.1.
YP_002956796.1. NC_012803.1.

3D structure databases

ProteinModelPortaliC5C9U0.
SMRiC5C9U0. Positions 5-726.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi465515.MlutN2_010100003623.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACS30242; ACS30242; Mlut_07100.
GeneIDi23700070.
KEGGimlu:Mlut_07100.
PATRICi22622057. VBIMicLut29563_0673.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OrthoDBiEOG6WT8CC.

Enzyme and pathway databases

BioCyciMLUT465515:GHH6-709-MONOMER.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR014069. pppGpp_PNP.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
TIGR02696. pppGpp_PNP. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 4698 / DSM 20030 / JCM 1464 / NBRC 3333 / NCIMB 9278 / NCTC 2665 / VKM Ac-2230.

Entry informationi

Entry nameiPNP_MICLC
AccessioniPrimary (citable) accession number: C5C9U0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: June 24, 2015
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.