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Protein

Urease subunit alpha

Gene

ureC

Organism
Micrococcus luteus (strain ATCC 4698 / DSM 20030 / JCM 1464 / NBRC 3333 / NCIMB 9278 / NCTC 2665 / VKM Ac-2230) (Micrococcus lysodeikticus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.UniRule annotation

Cofactori

Ni cationUniRule annotationNote: Binds 2 nickel ions per subunit.UniRule annotation

Pathwayi: urea degradation

This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Urease subunit beta (ureB), Urease subunit alpha (ureC), Urease subunit gamma (ureA)
This subpathway is part of the pathway urea degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (urease route), the pathway urea degradation and in Nitrogen metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi137 – 1371Nickel 1; via tele nitrogenUniRule annotation
Metal bindingi139 – 1391Nickel 1; via tele nitrogenUniRule annotation
Metal bindingi220 – 2201Nickel 1; via carbamate groupUniRule annotation
Metal bindingi220 – 2201Nickel 2; via carbamate groupUniRule annotation
Binding sitei222 – 2221SubstrateUniRule annotation
Metal bindingi249 – 2491Nickel 2; via pros nitrogenUniRule annotation
Metal bindingi275 – 2751Nickel 2; via tele nitrogenUniRule annotation
Active sitei323 – 3231Proton donorUniRule annotation
Metal bindingi363 – 3631Nickel 1UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Nickel

Enzyme and pathway databases

BioCyciMLUT465515:GHH6-343-MONOMER.
UniPathwayiUPA00258; UER00370.

Protein family/group databases

MEROPSiM38.982.

Names & Taxonomyi

Protein namesi
Recommended name:
Urease subunit alphaUniRule annotation (EC:3.5.1.5UniRule annotation)
Alternative name(s):
Urea amidohydrolase subunit alphaUniRule annotation
Gene namesi
Name:ureCUniRule annotation
Ordered Locus Names:Mlut_03440
OrganismiMicrococcus luteus (strain ATCC 4698 / DSM 20030 / JCM 1464 / NBRC 3333 / NCIMB 9278 / NCTC 2665 / VKM Ac-2230) (Micrococcus lysodeikticus)
Taxonomic identifieri465515 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesMicrococcaceaeMicrococcus
Proteomesi
  • UP000000738 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 571571Urease subunit alphaPRO_1000216198Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei220 – 2201N6-carboxylysineUniRule annotation

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two nickel ions.UniRule annotation

Interactioni

Subunit structurei

Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.UniRule annotation

Protein-protein interaction databases

STRINGi465515.MlutN2_010100005508.

Structurei

3D structure databases

ProteinModelPortaliC5C8U3.
SMRiC5C8U3. Positions 5-571.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini132 – 571440UreaseUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the urease family.UniRule annotation
Contains 1 urease domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQM. Bacteria.
COG0804. LUCA.
HOGENOMiHOG000075064.
KOiK01428.
OMAiFDSHIHF.
OrthoDBiEOG6ND0GM.

Family and domain databases

Gene3Di2.30.40.10. 2 hits.
HAMAPiMF_01953. Urease_alpha.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 1 hit.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C5C8U3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFEISREQY ASLYGPTTGD AIRLADTELF AVVEEDLTTP GEEAVFGGGK
60 70 80 90 100
VIRDGMGQNS QLVRDVGVPD LVITNVVVID WTGIYKADIA VRDAHIVAIG
110 120 130 140 150
KAGNPHTMDG VDIVIGVATD VISGEGKILT AGGIDTHVHF ISPDQIETAL
160 170 180 190 200
SSGLTTMIGG GTGPSESSKA TTITPGEWNI HTMLRSFEHW PMNFGLLGKG
210 220 230 240 250
HGSSISPMAE QIRAGAIGLK VHEDWGATPS SINTALQVAD EYDVQVAIHT
260 270 280 290 300
DTLNESGFVE DTRAAIDGRV IHTFHTEGAG GGHAPDIIEL AQYPNILPAS
310 320 330 340 350
TNPTLPYTTN TVEEHVDMLM VAHHLNADLP EDVAFADSRI RQETIAAEDV
360 370 380 390 400
LQDMGIFSMT SSDSQAMGRV GEVLIRTWQV ADSMKRQRGP LPEDEGTAGD
410 420 430 440 450
NHRIKRYVSK YTINPAIAHG IADSVGSVEV GKFADLVLWE PQFFGVKPDL
460 470 480 490 500
VIKGGQMVYG VVGDPNGSIP TPQPRWYRRS FGAYGQAVHT SAITFMSEAS
510 520 530 540 550
IRAGLPRALG LQKTIRAVHG IRDLTKADMR HNGETPRLEV DPETYEVRVD
560 570
GEPVTCEPQD VLPMAQRYFL F
Length:571
Mass (Da):61,595
Last modified:July 28, 2009 - v1
Checksum:i6085DF31D56DFE5E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001628 Genomic DNA. Translation: ACS29895.1.
RefSeqiWP_010079477.1. NZ_CABC01000068.1.

Genome annotation databases

EnsemblBacteriaiACS29895; ACS29895; Mlut_03440.
GeneIDi7986066.
KEGGimlu:Mlut_03440.
PATRICi22621331. VBIMicLut29563_0324.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001628 Genomic DNA. Translation: ACS29895.1.
RefSeqiWP_010079477.1. NZ_CABC01000068.1.

3D structure databases

ProteinModelPortaliC5C8U3.
SMRiC5C8U3. Positions 5-571.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi465515.MlutN2_010100005508.

Protein family/group databases

MEROPSiM38.982.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACS29895; ACS29895; Mlut_03440.
GeneIDi7986066.
KEGGimlu:Mlut_03440.
PATRICi22621331. VBIMicLut29563_0324.

Phylogenomic databases

eggNOGiENOG4105CQM. Bacteria.
COG0804. LUCA.
HOGENOMiHOG000075064.
KOiK01428.
OMAiFDSHIHF.
OrthoDBiEOG6ND0GM.

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.
BioCyciMLUT465515:GHH6-343-MONOMER.

Family and domain databases

Gene3Di2.30.40.10. 2 hits.
HAMAPiMF_01953. Urease_alpha.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 1 hit.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 4698 / DSM 20030 / JCM 1464 / NBRC 3333 / NCIMB 9278 / NCTC 2665 / VKM Ac-2230.

Entry informationi

Entry nameiURE1_MICLC
AccessioniPrimary (citable) accession number: C5C8U3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: November 11, 2015
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.