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Protein

Urease subunit alpha

Gene

ureC

Organism
Micrococcus luteus (strain ATCC 4698 / DSM 20030 / JCM 1464 / NBRC 3333 / NCIMB 9278 / NCTC 2665 / VKM Ac-2230) (Micrococcus lysodeikticus)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.UniRule annotation

Cofactori

Ni cationUniRule annotationNote: Binds 2 nickel ions per subunit.UniRule annotation

Pathwayi: urea degradation

This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Urease subunit beta (ureB), Urease subunit alpha (ureC), Urease subunit gamma (ureA)
This subpathway is part of the pathway urea degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (urease route), the pathway urea degradation and in Nitrogen metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi137Nickel 1; via tele nitrogenUniRule annotation1
Metal bindingi139Nickel 1; via tele nitrogenUniRule annotation1
Metal bindingi220Nickel 1; via carbamate groupUniRule annotation1
Metal bindingi220Nickel 2; via carbamate groupUniRule annotation1
Binding sitei222SubstrateUniRule annotation1
Metal bindingi249Nickel 2; via pros nitrogenUniRule annotation1
Metal bindingi275Nickel 2; via tele nitrogenUniRule annotation1
Active sitei323Proton donorUniRule annotation1
Metal bindingi363Nickel 1UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandMetal-binding, Nickel

Enzyme and pathway databases

BioCyciMLUT465515:G1GEW-337-MONOMER
UniPathwayiUPA00258; UER00370

Protein family/group databases

MEROPSiM38.982

Names & Taxonomyi

Protein namesi
Recommended name:
Urease subunit alphaUniRule annotation (EC:3.5.1.5UniRule annotation)
Alternative name(s):
Urea amidohydrolase subunit alphaUniRule annotation
Gene namesi
Name:ureCUniRule annotation
Ordered Locus Names:Mlut_03440
OrganismiMicrococcus luteus (strain ATCC 4698 / DSM 20030 / JCM 1464 / NBRC 3333 / NCIMB 9278 / NCTC 2665 / VKM Ac-2230) (Micrococcus lysodeikticus)
Taxonomic identifieri465515 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesMicrococcaceaeMicrococcus
Proteomesi
  • UP000000738 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002161981 – 571Urease subunit alphaAdd BLAST571

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei220N6-carboxylysineUniRule annotation1

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two nickel ions.UniRule annotation

Proteomic databases

PRIDEiC5C8U3

Interactioni

Subunit structurei

Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.UniRule annotation

Protein-protein interaction databases

STRINGi465515.MlutN2_010100005508

Structurei

3D structure databases

ProteinModelPortaliC5C8U3
SMRiC5C8U3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini132 – 571UreaseUniRule annotationAdd BLAST440

Sequence similaritiesi

Belongs to the metallo-dependent hydrolases superfamily. Urease alpha subunit family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQM Bacteria
COG0804 LUCA
HOGENOMiHOG000075064
KOiK01428
OMAiGFDSHIH
OrthoDBiPOG091H0ARR

Family and domain databases

CDDicd00375 Urease_alpha, 1 hit
Gene3Di2.30.40.10, 2 hits
HAMAPiMF_01953 Urease_alpha, 1 hit
InterProiView protein in InterPro
IPR006680 Amidohydro-rel
IPR011059 Metal-dep_hydrolase_composite
IPR032466 Metal_Hydrolase
IPR011612 Urease_alpha_N_dom
IPR005848 Urease_asu
IPR017951 Urease_asu_c
IPR029754 Urease_Ni-bd
PANTHERiPTHR43440 PTHR43440, 1 hit
PfamiView protein in Pfam
PF01979 Amidohydro_1, 1 hit
PF00449 Urease_alpha, 1 hit
PRINTSiPR01752 UREASE
SUPFAMiSSF51338 SSF51338, 1 hit
SSF51556 SSF51556, 2 hits
TIGRFAMsiTIGR01792 urease_alph, 1 hit
PROSITEiView protein in PROSITE
PS01120 UREASE_1, 1 hit
PS51368 UREASE_3, 1 hit

Sequencei

Sequence statusi: Complete.

C5C8U3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFEISREQY ASLYGPTTGD AIRLADTELF AVVEEDLTTP GEEAVFGGGK
60 70 80 90 100
VIRDGMGQNS QLVRDVGVPD LVITNVVVID WTGIYKADIA VRDAHIVAIG
110 120 130 140 150
KAGNPHTMDG VDIVIGVATD VISGEGKILT AGGIDTHVHF ISPDQIETAL
160 170 180 190 200
SSGLTTMIGG GTGPSESSKA TTITPGEWNI HTMLRSFEHW PMNFGLLGKG
210 220 230 240 250
HGSSISPMAE QIRAGAIGLK VHEDWGATPS SINTALQVAD EYDVQVAIHT
260 270 280 290 300
DTLNESGFVE DTRAAIDGRV IHTFHTEGAG GGHAPDIIEL AQYPNILPAS
310 320 330 340 350
TNPTLPYTTN TVEEHVDMLM VAHHLNADLP EDVAFADSRI RQETIAAEDV
360 370 380 390 400
LQDMGIFSMT SSDSQAMGRV GEVLIRTWQV ADSMKRQRGP LPEDEGTAGD
410 420 430 440 450
NHRIKRYVSK YTINPAIAHG IADSVGSVEV GKFADLVLWE PQFFGVKPDL
460 470 480 490 500
VIKGGQMVYG VVGDPNGSIP TPQPRWYRRS FGAYGQAVHT SAITFMSEAS
510 520 530 540 550
IRAGLPRALG LQKTIRAVHG IRDLTKADMR HNGETPRLEV DPETYEVRVD
560 570
GEPVTCEPQD VLPMAQRYFL F
Length:571
Mass (Da):61,595
Last modified:July 28, 2009 - v1
Checksum:i6085DF31D56DFE5E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001628 Genomic DNA Translation: ACS29895.1
RefSeqiWP_010079477.1, NZ_CABC01000068.1

Genome annotation databases

EnsemblBacteriaiACS29895; ACS29895; Mlut_03440
GeneIDi7986066
KEGGimlu:Mlut_03440
PATRICifig|465515.4.peg.324

Similar proteinsi

Entry informationi

Entry nameiURE1_MICLC
AccessioniPrimary (citable) accession number: C5C8U3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: May 23, 2018
This is version 55 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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