C5C697 (FMT_BEUC1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionyl-tRNA formyltransferase
EC=2.1.2.9

Gene names

Name:	fmt
Ordered Locus Names:	Bcav_2048

Organism

Beutenbergia cavernae (strain ATCC BAA-8 / DSM 12333 / NBRC 16432) [Complete proteome] [HAMAP]

Taxonomic identifier

471853 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Beutenbergiaceae › Beutenbergia › Beutenbergia cavernae

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by: (I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-GTP By similarity. HAMAP-Rule MF_00182
Catalytic activity	10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet). HAMAP-Rule MF_00182
Sequence similarities	Belongs to the Fmt family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translational initiation Inferred from electronic annotation. Source: GOC
Molecular_function	methionyl-tRNA formyltransferase activity Inferred from electronic annotation. Source: HAMAP methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Methionyl-tRNA formyltransferase HAMAP-Rule MF_00182		PRO_1000203848
Regions
	Region	109 – 112	4	Tetrahydrofolate (THF) binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	C5C697 [UniParc]. Last modified July 28, 2009. Version 1. Checksum: 1458CDB4B5CA0171	FASTA	311	32,670
	10 20 30 40 50 60 MRVLFAGTPD VAIPTLRALV GSRHHVVAAL TRPPARRSRR GHDEPSPVAA TAAELGVPVL 70 80 90 100 110 120 EPRSVRATDV AEELRALDLD VAVVVAYGAL LPEDLLAIPR HGWINLHFSV LPAWRGAAPV 130 140 150 160 170 180 QHAVWHGDEV TGATTFRITA GLDEGPVYGV LTERVRPRDT SGDLLARLAD AGPRLVLDTL 190 200 210 220 230 240 DAVADGTARP QAQPDDGVTL APRLSVEQAR VRWADPALAV DRQVRACTPA PGAWTTLDGA 250 260 270 280 290 300 RVKVGPLDPV DAPPLPAGRI AVERQRVLVG TGSAPVELGR LAPAGKGWMD AASWARGARL 310 AGDATFGADD G

References

[1]

"Complete genome sequence of Beutenbergia cavernae type strain (HKI 0122)." Land M., Pukall R., Abt B., Goker M., Rohde M., Glavina Del Rio T., Tice H., Copeland A., Cheng J.F., Lucas S., Chen F., Nolan M., Bruce D., Goodwin L., Pitluck S., Ivanova N., Mavromatis K., Ovchinnikova G. , Pati A., Chen A., Palaniappan K., Hauser L., Chang Y.J., Jefferies C.C., Saunders E., Brettin T., Detter J.C., Han C., Chain P., Bristow J., Eisen J.A., Markowitz V., Hugenholtz P., Kyrpides N.C., Klenk H.P., Lapidus A. Stand. Genomic Sci. 1:21-28(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-8 / DSM 12333 / NBRC 16432.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001618 Genomic DNA. Translation: ACQ80303.1.
RefSeq	YP_002882065.1. NC_012669.1.
3D structure databases
ProteinModelPortal	C5C697.
ModBase	Search...
Protein-protein interaction databases
STRING	471853.Bcav_2048.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACQ80303; ACQ80303; Bcav_2048.
GeneID	7859328.
KEGG	bcv:Bcav_2048.
PATRIC	21094754. VBIBeuCav91323_2089.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0223.
HOGENOM	HOG000261177.
KO	K00604.
OMA	GITLMQM.
Enzyme and pathway databases
BioCyc	BCAV471853:GI1Z-2078-MONOMER.
Family and domain databases
Gene3D	3.10.25.10. 1 hit. 3.40.50.170. 1 hit.
HAMAP	MF_00182. Formyl_trans.
InterPro	IPR005794. Fmt. IPR005793. Formyl_trans_C. IPR002376. Formyl_transf_N. IPR011034. Formyl_transferase_C-like. IPR015518. Met_tRNA_Form_TA-like. [Graphical view]
PANTHER	PTHR11138. PTHR11138. 1 hit.
Pfam	PF02911. Formyl_trans_C. 1 hit. PF00551. Formyl_trans_N. 1 hit. [Graphical view]
SUPFAM	SSF50486. FMT_C_like. 1 hit. SSF53328. formyl_transf. 1 hit.
TIGRFAMs	TIGR00460. fmt. 1 hit.
PROSITE	PS00373. GART. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FMT_BEUC1

Accession

Primary (citable) accession number: C5C697

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	July 28, 2009
Last modified:	May 29, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program