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C5BQX2

- SYI_TERTT

UniProt

C5BQX2 - SYI_TERTT

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Protein

Isoleucine--tRNA ligase

Gene
ileS, TERTU_1040
Organism
Teredinibacter turnerae (strain ATCC 39867 / T7901)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei559 – 5591Aminoacyl-adenylate By similarity
Binding sitei603 – 6031ATP By similarity
Metal bindingi897 – 8971Zinc By similarity
Metal bindingi900 – 9001Zinc By similarity
Metal bindingi917 – 9171Zinc By similarity
Metal bindingi920 – 9201Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciTTUR377629:GHSU-945-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:TERTU_1040
OrganismiTeredinibacter turnerae (strain ATCC 39867 / T7901)
Taxonomic identifieri377629 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadales genera incertae sedisTeredinibacter
ProteomesiUP000009080: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 934934Isoleucine--tRNA ligaseUniRule annotationPRO_1000216247Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi377629.TERTU_1040.

Structurei

3D structure databases

ProteinModelPortaliC5BQX2.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotationAdd
BLAST
Motifi600 – 6045"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C5BQX2-1 [UniParc]FASTAAdd to Basket

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MTDYKSTLNL PQTSFAMKAN LAQREPQTLK RWQKENLYQQ IRQARAGREK    50
FILHDGPPYA NGEIHIGHAV NKILKDIIVK AKTLSGFDAP YIPGWDCHGL 100
PIEHNVEKKV GKAGVKVDFA TFRKKCREYA AKQVAGQKEG FVRLGVLADW 150
DKPYLTMDYK TEADIVRALG KIVANGHLVR GFKPVYWSVV GGSALAEAEV 200
EYQEKTSFSI DVKYAVKDEA DFTQRVAELG GEGPVSVVIW TTTPWTLPSS 250
QAVSLNADLE YVVVQQPGAR LLVAEALLES VGKRAGIDTA TIVGRCHGRD 300
LENLVLQHPF YTREVPVILG DHVTTDAGTG CVHTAPDHGM EDFEVGSRYG 350
IGTLNYVDEN GLYRESVEIF AGDHVYKVDE KIIELLESRD ALLHQEKFTH 400
SYPHCWRTKT PLIFRATPQW FISMTKNGLL DTVKTAVDGV EWIPDWGEAR 450
MRSMLEASPD WCISRQRTWG VPIALFVHKE TQDLHPDTPA LVEKVAALIE 500
TDGMDAWFNL NPEEILGEDA ANYSKVTDTL DVWFDSGVTH YSVIQQREEL 550
QYPADLYLEG SDQHRGWFQS SLKTAIAING TAPYKQVLTH GFTVDANGKK 600
MSKSIGNTVS PQKVMNELGA DVLRLWVAAT DFSGDMSVSD EILMRTADSY 650
RRIRNTMRYF MSNLNGFDPA INSVAFDDMV ALDRWAVDRA AKLQRDIVAC 700
YDSYQFHTIY QKIHNFCIVD MGGFYLDIIK DRVYTMQEDS RARRSAQTAQ 750
YLIVQALVRW IAPILSFTAD EIWQALPGEK TGPVFVAEWL ELPELSEDDA 800
LNNSYWQTAA KVKTAVNKVL ESKRSSGVIG GSLGAEVTLY ASDELHAKLN 850
SLGEELRFVL LVSAVNLKKL DEAPEDADQV DVVGLKVAVT KSEAAKCARC 900
WHQRDDVGSH SEHPELCGRC VSNVEGNGEV RHYA 934
Length:934
Mass (Da):104,465
Last modified:July 28, 2009 - v1
Checksum:i4329BDB88BC66E91
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001614 Genomic DNA. Translation: ACR11703.1.
RefSeqiWP_015817815.1. NC_012997.1.
YP_003072635.1. NC_012997.1.

Genome annotation databases

EnsemblBacteriaiACR11703; ACR11703; TERTU_1040.
GeneIDi8211008.
KEGGittu:TERTU_1040.
PATRICi23869259. VBITerTur118718_0982.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001614 Genomic DNA. Translation: ACR11703.1 .
RefSeqi WP_015817815.1. NC_012997.1.
YP_003072635.1. NC_012997.1.

3D structure databases

ProteinModelPortali C5BQX2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 377629.TERTU_1040.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACR11703 ; ACR11703 ; TERTU_1040 .
GeneIDi 8211008.
KEGGi ttu:TERTU_1040.
PATRICi 23869259. VBITerTur118718_0982.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci TTUR377629:GHSU-945-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39867 / T7901.

Entry informationi

Entry nameiSYI_TERTT
AccessioniPrimary (citable) accession number: C5BQX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: September 3, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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