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C5BQX2

- SYI_TERTT

UniProt

C5BQX2 - SYI_TERTT

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Teredinibacter turnerae (strain ATCC 39867 / T7901)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 41 (01 Oct 2014)
      Sequence version 1 (28 Jul 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei559 – 5591Aminoacyl-adenylateUniRule annotation
    Binding sitei603 – 6031ATPUniRule annotation
    Metal bindingi897 – 8971ZincUniRule annotation
    Metal bindingi900 – 9001ZincUniRule annotation
    Metal bindingi917 – 9171ZincUniRule annotation
    Metal bindingi920 – 9201ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciTTUR377629:GHSU-945-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:TERTU_1040
    OrganismiTeredinibacter turnerae (strain ATCC 39867 / T7901)
    Taxonomic identifieri377629 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadales genera incertae sedisTeredinibacter
    ProteomesiUP000009080: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 934934Isoleucine--tRNA ligasePRO_1000216247Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi377629.TERTU_1040.

    Structurei

    3D structure databases

    ProteinModelPortaliC5BQX2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi600 – 6045"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    C5BQX2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDYKSTLNL PQTSFAMKAN LAQREPQTLK RWQKENLYQQ IRQARAGREK    50
    FILHDGPPYA NGEIHIGHAV NKILKDIIVK AKTLSGFDAP YIPGWDCHGL 100
    PIEHNVEKKV GKAGVKVDFA TFRKKCREYA AKQVAGQKEG FVRLGVLADW 150
    DKPYLTMDYK TEADIVRALG KIVANGHLVR GFKPVYWSVV GGSALAEAEV 200
    EYQEKTSFSI DVKYAVKDEA DFTQRVAELG GEGPVSVVIW TTTPWTLPSS 250
    QAVSLNADLE YVVVQQPGAR LLVAEALLES VGKRAGIDTA TIVGRCHGRD 300
    LENLVLQHPF YTREVPVILG DHVTTDAGTG CVHTAPDHGM EDFEVGSRYG 350
    IGTLNYVDEN GLYRESVEIF AGDHVYKVDE KIIELLESRD ALLHQEKFTH 400
    SYPHCWRTKT PLIFRATPQW FISMTKNGLL DTVKTAVDGV EWIPDWGEAR 450
    MRSMLEASPD WCISRQRTWG VPIALFVHKE TQDLHPDTPA LVEKVAALIE 500
    TDGMDAWFNL NPEEILGEDA ANYSKVTDTL DVWFDSGVTH YSVIQQREEL 550
    QYPADLYLEG SDQHRGWFQS SLKTAIAING TAPYKQVLTH GFTVDANGKK 600
    MSKSIGNTVS PQKVMNELGA DVLRLWVAAT DFSGDMSVSD EILMRTADSY 650
    RRIRNTMRYF MSNLNGFDPA INSVAFDDMV ALDRWAVDRA AKLQRDIVAC 700
    YDSYQFHTIY QKIHNFCIVD MGGFYLDIIK DRVYTMQEDS RARRSAQTAQ 750
    YLIVQALVRW IAPILSFTAD EIWQALPGEK TGPVFVAEWL ELPELSEDDA 800
    LNNSYWQTAA KVKTAVNKVL ESKRSSGVIG GSLGAEVTLY ASDELHAKLN 850
    SLGEELRFVL LVSAVNLKKL DEAPEDADQV DVVGLKVAVT KSEAAKCARC 900
    WHQRDDVGSH SEHPELCGRC VSNVEGNGEV RHYA 934
    Length:934
    Mass (Da):104,465
    Last modified:July 28, 2009 - v1
    Checksum:i4329BDB88BC66E91
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001614 Genomic DNA. Translation: ACR11703.1.
    RefSeqiWP_015817815.1. NC_012997.1.
    YP_003072635.1. NC_012997.1.

    Genome annotation databases

    EnsemblBacteriaiACR11703; ACR11703; TERTU_1040.
    GeneIDi8211008.
    KEGGittu:TERTU_1040.
    PATRICi23869259. VBITerTur118718_0982.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001614 Genomic DNA. Translation: ACR11703.1 .
    RefSeqi WP_015817815.1. NC_012997.1.
    YP_003072635.1. NC_012997.1.

    3D structure databases

    ProteinModelPortali C5BQX2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 377629.TERTU_1040.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACR11703 ; ACR11703 ; TERTU_1040 .
    GeneIDi 8211008.
    KEGGi ttu:TERTU_1040.
    PATRICi 23869259. VBITerTur118718_0982.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci TTUR377629:GHSU-945-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 39867 / T7901.

    Entry informationi

    Entry nameiSYI_TERTT
    AccessioniPrimary (citable) accession number: C5BQX2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: July 28, 2009
    Last modified: October 1, 2014
    This is version 41 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3