C5BGI8 (SURE_EDWI9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5'/3'-nucleotidase SurE
EC=3.1.3.5
EC=3.1.3.6

Alternative name(s):
Exopolyphosphatase
EC=3.6.1.11
Nucleoside monophosphate phosphohydrolase

Gene names

Name:	surE
Ordered Locus Names:	NT01EI_3253

Organism

Edwardsiella ictaluri (strain 93-146) [Complete proteome] [HAMAP]

Taxonomic identifier

634503 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Edwardsiella › Edwardsiella ictaluri

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Nucleotidase with a broad substrate specificity as it can dephosphorylate various ribo- and deoxyribonucleoside 5'-monophosphates and ribonucleoside 3'-monophosphates with highest affinity to 3'-AMP. Also hydrolyzes polyphosphate (exopolyphosphatase activity) with the preference for short-chain-length substrates (P20-25). Might be involved in the regulation of dNTP and NTP pools, and in the turnover of 3'-mononucleotides produced by numerous intracellular RNases (T1, T2, and F) during the degradation of various RNAs By similarity. HAMAP-Rule MF_00060
Catalytic activity	A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate. HAMAP-Rule MF_00060 A 3'-ribonucleotide + H2O = a ribonucleoside + phosphate. HAMAP-Rule MF_00060 (Polyphosphate)(n) + H2O = (polyphosphate)(n-1) + phosphate. HAMAP-Rule MF_00060
Cofactor	Binds 1 divalent metal cation per subunit By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00060.
Sequence similarities	Belongs to the SurE nucleotidase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	3'-nucleotidase activity Inferred from electronic annotation. Source: HAMAP 5'-nucleotidase activity Inferred from electronic annotation. Source: HAMAP exopolyphosphatase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 254	254	5'/3'-nucleotidase SurE HAMAP-Rule MF_00060		PRO_1000202367
Sites
	Metal binding	8	1	Divalent metal cation By similarity
	Metal binding	9	1	Divalent metal cation By similarity
	Metal binding	39	1	Divalent metal cation By similarity
	Metal binding	92	1	Divalent metal cation By similarity

Sequences

	Sequence		Length	Mass (Da)
	C5BGI8 [UniParc]. Last modified July 28, 2009. Version 1. Checksum: C60B2E2900A4D68B	FASTA	254	26,839
	10 20 30 40 50 60 MRILLSNDDG VTAPGIQTLA AALREFAQVQ VVAPNRNRSG SSNALTLESP LRSETLANGD 70 80 90 100 110 120 ISVIDGTPTD CVYLGVNALM RPRPDIVIAG INAGPNLGDD VIYSGTVAAA MEGRHLGFPA 130 140 150 160 170 180 LAVSLDGERH YDTAAAVTCR LLRMLSDAPL RSGRILNVNV PDVPLTAIRG WRVTRCGSRH 190 200 210 220 230 240 PAQTVIHQQD PRGKPLMWIG PPGAKQDAGE ETDFAAVAAG YISVTPLQVD LTAHGARGRL 250 AEWLGRVDKG GAAW

References

[1]

"Complete genome sequence of Edwardsiella ictaluri 93-146." Williams M.L., Gillaspy A.F., Dyer D.W., Thune R.L., Waldbieser G.C., Schuster S.C., Gipson J., Zaitshik J., Landry C., Lawrence M.L. Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 93-146.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001600 Genomic DNA. Translation: ACR70394.1.
RefSeq	YP_002934629.1. NC_012779.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	634503.NT01EI_3253.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACR70394; ACR70394; NT01EI_3253.
GeneID	7961086.
KEGG	eic:NT01EI_3253.
PATRIC	21839276. VBIEdwIct114273_2899.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0496.
HOGENOM	HOG000122500.
KO	K03787.
OMA	LGSNMGE.
ProtClustDB	PRK00346.
Enzyme and pathway databases
BioCyc	EICT634503:GCMY-3227-MONOMER.
Family and domain databases
Gene3D	3.40.1210.10. 1 hit.
HAMAP	MF_00060. SurE.
InterPro	IPR002828. SurE-like_Pase/nucleotidase. [Graphical view]
Pfam	PF01975. SurE. 1 hit. [Graphical view]
SUPFAM	SSF64167. SurE-like_Pase/nucleotidase. 1 hit.
TIGRFAMs	TIGR00087. surE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SURE_EDWI9

Accession

Primary (citable) accession number: C5BGI8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	July 28, 2009
Last modified:	May 29, 2013
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program