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C5BGC7 (SYL_EDWI9) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:NT01EI_2936
OrganismEdwardsiella ictaluri (strain 93-146) [Complete proteome] [HAMAP]
Taxonomic identifier634503 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEdwardsiella

Protein attributes

Sequence length860 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 860860Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000202219

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif619 – 6235"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6221ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
C5BGC7 [UniParc].

Last modified July 28, 2009. Version 1.
Checksum: 913E815D3AC84093

FASTA86097,160
        10         20         30         40         50         60 
MQEQYRPEDI ESHVQRHWDE QKTFQVTEDA GKEKYYCLSM LPYPSGRLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VISRYQRMLG KNVLQPIGWD AFGLPAEGAA VKNNTAPAPW TYANIDYMKN QLKLLGFGYD 

       130        140        150        160        170        180 
WSREIATCKP DYYRWEQWFF TKLYEKGLVY KKTSAVNWCP NDQTVLANEQ VIDGCCWRCD 

       190        200        210        220        230        240 
SKVERKEIPQ WFIKITDYAD QLLNDLDRLE EWPEQVKTMQ RNWIGRSEGV EITFHVADRD 

       250        260        270        280        290        300 
DTFAVYTTRP DTFMGVSYLA IAAAHPLAQQ AATGNPALTQ FIDECKNTKV AEADMATMEK 

       310        320        330        340        350        360 
KGMATGLYAI HPLNGERLPI WVANFVLMDY GTGAVMSVPA HDQRDWEFAT RYDLPMKPVI 

       370        380        390        400        410        420 
LTADGQAPDI RAAAMTDKGV LFNSGEFDGL DFSAAFDAVA NRLIAAGVGE RKVNYRLRDW 

       430        440        450        460        470        480 
GVSRQRYWGA PIPMMTLEDG SVIPTPEEQL PVILPEDVVM NGITSPIKAD PSWAKTTVNG 

       490        500        510        520        530        540 
QPALRETDTF DTFMESSWYY ARYTCPQFDQ GMLDPQAANY WLPVDQYVGG IEHAIMHLMY 

       550        560        570        580        590        600 
FRFFHKLMRD AGLVDSDEPA KRLLCQGMVL ADAFYYTGTN GERNWVSPVD VTVERDDKGR 

       610        620        630        640        650        660 
ITQATDRDGR ELVYAGMSKM SKSKNNGIDP QVMVERYGAD TVRLFMMFAS PAEMTLEWQE 

       670        680        690        700        710        720 
SGVEGANRFL KRVWKLAFDH QQKGLASALD LTALNDDQKA LRRDLHKTIA KVSDDIGRRQ 

       730        740        750        760        770        780 
TFNTAIAAVM ELMNKLTRAP QESEQDRALM QEALLAVVRM LYPFTPHVCF TLWRALGGAG 

       790        800        810        820        830        840 
DIDTAPWPVA DDAAMVEDSK LIVVQVNGKV RGKITVAADA SEEQVRALAA QEPLVAKYLD 

       850        860 
GVTVRKVIFV PGKLLNLVVG 

« Hide

References

[1]"Complete genome sequence of Edwardsiella ictaluri 93-146."
Williams M.L., Gillaspy A.F., Dyer D.W., Thune R.L., Waldbieser G.C., Schuster S.C., Gipson J., Zaitshik J., Landry C., Lawrence M.L.
Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 93-146.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001600 Genomic DNA. Translation: ACR70090.1.
RefSeqYP_002934325.1. NC_012779.2.

3D structure databases

ProteinModelPortalC5BGC7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING634503.NT01EI_2936.

Proteomic databases

PRIDEC5BGC7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACR70090; ACR70090; NT01EI_2936.
GeneID7960214.
KEGGeic:NT01EI_2936.
PATRIC21838704. VBIEdwIct114273_2623.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycEICT634503:GCMY-2912-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_EDWI9
AccessionPrimary (citable) accession number: C5BGC7
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: May 14, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries