C5BFM6 (NAGZ_EDWI9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-hexosaminidase
EC=3.2.1.52

Alternative name(s):
Beta-N-acetylhexosaminidase
N-acetyl-beta-glucosaminidase

Gene names

Name:	nagZ
Ordered Locus Names:	NT01EI_1812

Organism

Edwardsiella ictaluri (strain 93-146) [Complete proteome] [HAMAP]

Taxonomic identifier

634503 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Edwardsiella › Edwardsiella ictaluri

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves GlcNAc linked beta-1,4 to MurNAc tripeptides By similarity. HAMAP-Rule MF_00364
Catalytic activity	Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. HAMAP-Rule MF_00364
Pathway	Cell wall biogenesis; peptidoglycan recycling. HAMAP-Rule MF_00364
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 3 family. NagZ subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan turnover Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	beta-N-acetylhexosaminidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Beta-hexosaminidase HAMAP-Rule MF_00364		PRO_1000205460
Sites
	Active site	248	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	C5BFM6 [UniParc]. Last modified July 28, 2009. Version 1. Checksum: 97CC6A724CAD7F80	FASTA	340	37,276
	10 20 30 40 50 60 MGPVMLDVVG HELDAEEREI LDHPLVGGVI LFSRNFDDPA QLRELVRQIR QAARHPLLVT 70 80 90 100 110 120 VDQEGGRVQR FHQGFTRLPA AQSYAALNTP QEAQRLALEG GWLMACEMIA MDIDLSFAPV 130 140 150 160 170 180 LDLGHGSAAI GERAFHREAE SAIPLAQAFI DGMHQAGMKA TGKHFPGHGK VSADSHKETP 190 200 210 220 230 240 IDARPLVQIV EHDMVIFRTL NACGKLDAVM PAHVIYSQAD SRPASGSPYW LQQVLRQTLG 250 260 270 280 290 300 FEGVVFSDDL SMNGAAVMGS YAQRAQASLD AGCDMVLVCN NRQGAISVLD HLSAAGSPRI 310 320 330 340 ARLRHRGQVD REGLMGSARW KQAHQDLQRL HQRWEEAKQA

References

[1]

"Complete genome sequence of Edwardsiella ictaluri 93-146." Williams M.L., Gillaspy A.F., Dyer D.W., Thune R.L., Waldbieser G.C., Schuster S.C., Gipson J., Zaitshik J., Landry C., Lawrence M.L. Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 93-146.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001600 Genomic DNA. Translation: ACR68989.1.
RefSeq	YP_002933224.1. NC_012779.2.
3D structure databases
ProteinModelPortal	C5BFM6.
ModBase	Search...
Protein-protein interaction databases
STRING	634503.NT01EI_1812.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACR68989; ACR68989; NT01EI_1812.
GeneID	7962133.
KEGG	eic:NT01EI_1812.
PATRIC	21836670. VBIEdwIct114273_1625.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1472.
HOGENOM	HOG000248526.
KO	K01207.
OMA	VVLHCNG.
ProtClustDB	PRK05337.
Enzyme and pathway databases
UniPathway	UPA00544.
Family and domain databases
Gene3D	3.20.20.300. 1 hit.
HAMAP	MF_00364. NagZ.
InterPro	IPR022956. Beta_hexosaminidase_bac. IPR001764. Glyco_hydro_3_N. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00933. Glyco_hydro_3. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00775. GLYCOSYL_HYDROL_F3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAGZ_EDWI9

Accession

Primary (citable) accession number: C5BFM6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	July 28, 2009
Last modified:	May 1, 2013
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program