Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

C5BCF2 (TRMB_EDWI9) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:NT01EI_0273
OrganismEdwardsiella ictaluri (strain 93-146) [Complete proteome] [HAMAP]
Taxonomic identifier634503 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEdwardsiella

Protein attributes

Sequence length238 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Subunit structure

Monomer By similarity. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 238238tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_1000213439

Regions

Region216 – 2194Substrate binding By similarity

Sites

Active site1431 By similarity
Binding site681S-adenosyl-L-methionine By similarity
Binding site931S-adenosyl-L-methionine By similarity
Binding site1201S-adenosyl-L-methionine By similarity
Binding site1431S-adenosyl-L-methionine By similarity
Binding site1471Substrate By similarity
Binding site1791Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
C5BCF2 [UniParc].

Last modified July 28, 2009. Version 1.
Checksum: BB6313E76A4A4802

FASTA23827,086
        10         20         30         40         50         60 
MNDVITAEFD ENGRAIRRIR SFVRRQGRLT KGQQLALDEY WPLMGVEFTP EALDLPTLFG 

        70         80         90        100        110        120 
REAPVVLEIG FGMGASLVAM AQQHPERDFL GIEVHSPGVG ACLSSAHEAG VNNLRVMCHD 

       130        140        150        160        170        180 
AVEVLERMIP DASLDMVQLF FPDPWHKARH HKRRIVQSAF AGRIRGKLKI SGVFHMATDW 

       190        200        210        220        230 
ENYAEHMLEV MNHAPGYRNL SDDNTYVPRP DSRPVTKFEL RGQRLGHGNW DLMFERVE 

« Hide

References

[1]"Complete genome sequence of Edwardsiella ictaluri 93-146."
Williams M.L., Gillaspy A.F., Dyer D.W., Thune R.L., Waldbieser G.C., Schuster S.C., Gipson J., Zaitshik J., Landry C., Lawrence M.L.
Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 93-146.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001600 Genomic DNA. Translation: ACR67515.1.
RefSeqYP_002931750.1. NC_012779.2.

3D structure databases

ProteinModelPortalC5BCF2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING634503.NT01EI_0273.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACR67515; ACR67515; NT01EI_0273.
GeneID7960053.
KEGGeic:NT01EI_0273.
PATRIC21833818. VBIEdwIct114273_0245.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMAHSTLEMA.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycEICT634503:GCMY-272-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_EDWI9
AccessionPrimary (citable) accession number: C5BCF2
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: June 11, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways