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C5BC46

- APHA_EDWI9

UniProt

C5BC46 - APHA_EDWI9

Protein

Class B acid phosphatase

Gene

aphA

Organism
Edwardsiella ictaluri (strain 93-146)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 33 (01 Oct 2014)
      Sequence version 1 (28 Jul 2009)
      Previous versions | rss
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    Functioni

    Dephosphorylates several organic phosphate monoesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds By similarity.By similarity

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.By similarity

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei72 – 721NucleophileBy similarity
    Metal bindingi72 – 721MagnesiumBy similarity
    Active sitei74 – 741Proton donorBy similarity
    Metal bindingi74 – 741Magnesium; via carbonyl oxygenBy similarity
    Binding sitei181 – 1811SubstrateBy similarity
    Metal bindingi196 – 1961MagnesiumBy similarity

    GO - Molecular functioni

    1. acid phosphatase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciEICT634503:GCMY-3809-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Class B acid phosphataseBy similarity (EC:3.1.3.2By similarity)
    Short name:
    CBAPBy similarity
    Gene namesi
    Name:aphABy similarity
    Ordered Locus Names:NT01EI_3833
    OrganismiEdwardsiella ictaluri (strain 93-146)
    Taxonomic identifieri634503 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEdwardsiella
    ProteomesiUP000001485: Chromosome

    Subcellular locationi

    Periplasm By similarity

    GO - Cellular componenti

    1. outer membrane-bounded periplasmic space Source: InterPro

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Sequence AnalysisAdd
    BLAST
    Chaini28 – 241214Class B acid phosphataseSequence AnalysisPRO_0000415225Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi634503.NT01EI_3833.

    Structurei

    3D structure databases

    ProteinModelPortaliC5BC46.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni141 – 1422Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the class B bacterial acid phosphatase family.By similarity

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3700.
    HOGENOMiHOG000270623.
    KOiK03788.
    OMAiPEFWEKM.
    OrthoDBiEOG6HTNVZ.

    Family and domain databases

    Gene3Di3.40.50.1000. 1 hit.
    InterProiIPR005519. Acid_phosphat_B.
    IPR023214. HAD-like_dom.
    IPR010025. HAD-SF_ppase_IIIB_AphA.
    [Graphical view]
    PfamiPF03767. Acid_phosphat_B. 1 hit.
    [Graphical view]
    PIRSFiPIRSF017818. Acid_Ptase_B. 1 hit.
    SUPFAMiSSF56784. SSF56784. 1 hit.
    TIGRFAMsiTIGR01672. AphA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    C5BC46-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFITTKKSLI ALVLATAGLI SSPVSFATET GAQPNLGATL TQLTQQYPIH    50
    WISIEQVAES LKGKAPISVG FDIDDTLLFS SPAFFYGKQK FSPDSNAFLK 100
    NQKFWDAVSS SGWDRFSIPK DSGRALMELH LKRGDHVYFI TGRPMPSNGK 150
    EDLTQTLKDD FKIPDNQLNK VIFAGTKQDA KVEYMQKYHI TIFYGDSDND 200
    IQDARKAGAE GIRVLRPLNS TNKPMPKNGA FGEKVIVNSQ Y 241
    Length:241
    Mass (Da):26,760
    Last modified:July 28, 2009 - v1
    Checksum:i1BD08167680BA319
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001600 Genomic DNA. Translation: ACR70952.1.
    RefSeqiWP_015872984.1. NC_012779.2.
    YP_002935187.1. NC_012779.2.

    Genome annotation databases

    EnsemblBacteriaiACR70952; ACR70952; NT01EI_3833.
    GeneIDi7959874.
    KEGGieic:NT01EI_3833.
    PATRICi21840359. VBIEdwIct114273_3420.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001600 Genomic DNA. Translation: ACR70952.1 .
    RefSeqi WP_015872984.1. NC_012779.2.
    YP_002935187.1. NC_012779.2.

    3D structure databases

    ProteinModelPortali C5BC46.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 634503.NT01EI_3833.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACR70952 ; ACR70952 ; NT01EI_3833 .
    GeneIDi 7959874.
    KEGGi eic:NT01EI_3833.
    PATRICi 21840359. VBIEdwIct114273_3420.

    Phylogenomic databases

    eggNOGi COG3700.
    HOGENOMi HOG000270623.
    KOi K03788.
    OMAi PEFWEKM.
    OrthoDBi EOG6HTNVZ.

    Enzyme and pathway databases

    BioCyci EICT634503:GCMY-3809-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.1000. 1 hit.
    InterProi IPR005519. Acid_phosphat_B.
    IPR023214. HAD-like_dom.
    IPR010025. HAD-SF_ppase_IIIB_AphA.
    [Graphical view ]
    Pfami PF03767. Acid_phosphat_B. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF017818. Acid_Ptase_B. 1 hit.
    SUPFAMi SSF56784. SSF56784. 1 hit.
    TIGRFAMsi TIGR01672. AphA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of Edwardsiella ictaluri 93-146."
      Williams M.L., Gillaspy A.F., Dyer D.W., Thune R.L., Waldbieser G.C., Schuster S.C., Gipson J., Zaitshik J., Landry C., Lawrence M.L.
      Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 93-1461 Publication.

    Entry informationi

    Entry nameiAPHA_EDWI9
    AccessioniPrimary (citable) accession number: C5BC46
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 25, 2012
    Last sequence update: July 28, 2009
    Last modified: October 1, 2014
    This is version 33 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3