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Protein

Class B acid phosphatase

Gene

aphA

Organism
Edwardsiella ictaluri (strain 93-146)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Dephosphorylates several organic phosphate monoesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds (By similarity).By similarity

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.By similarity

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei72NucleophileBy similarity1
Metal bindingi72MagnesiumBy similarity1
Active sitei74Proton donorBy similarity1
Metal bindingi74Magnesium; via carbonyl oxygenBy similarity1
Binding sitei181SubstrateBy similarity1
Metal bindingi196MagnesiumBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Class B acid phosphataseBy similarity (EC:3.1.3.2By similarity)
Short name:
CBAPBy similarity
Gene namesi
Name:aphABy similarity
Ordered Locus Names:NT01EI_3833
OrganismiEdwardsiella ictaluri (strain 93-146)
Taxonomic identifieri634503 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesHafniaceaeEdwardsiella
Proteomesi
  • UP000001485 Componenti: Chromosome

Subcellular locationi

  • Periplasm By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000041522528 – 241Class B acid phosphataseSequence analysisAdd BLAST214

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi634503.NT01EI_3833.

Structurei

3D structure databases

ProteinModelPortaliC5BC46.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni141 – 142Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the class B bacterial acid phosphatase family.By similarity

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105F0S. Bacteria.
COG3700. LUCA.
HOGENOMiHOG000270623.
KOiK03788.
OMAiPEFWEKM.
OrthoDBiPOG091H06BK.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR005519. Acid_phosphat_B-like.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamiPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01672. AphA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

C5BC46-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFITTKKSLI ALVLATAGLI SSPVSFATET GAQPNLGATL TQLTQQYPIH
60 70 80 90 100
WISIEQVAES LKGKAPISVG FDIDDTLLFS SPAFFYGKQK FSPDSNAFLK
110 120 130 140 150
NQKFWDAVSS SGWDRFSIPK DSGRALMELH LKRGDHVYFI TGRPMPSNGK
160 170 180 190 200
EDLTQTLKDD FKIPDNQLNK VIFAGTKQDA KVEYMQKYHI TIFYGDSDND
210 220 230 240
IQDARKAGAE GIRVLRPLNS TNKPMPKNGA FGEKVIVNSQ Y
Length:241
Mass (Da):26,760
Last modified:July 28, 2009 - v1
Checksum:i1BD08167680BA319
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001600 Genomic DNA. Translation: ACR70952.1.
RefSeqiWP_015872984.1. NC_012779.2.

Genome annotation databases

EnsemblBacteriaiACR70952; ACR70952; NT01EI_3833.
GeneIDi7959874.
KEGGieic:NT01EI_3833.
PATRICi21840359. VBIEdwIct114273_3420.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001600 Genomic DNA. Translation: ACR70952.1.
RefSeqiWP_015872984.1. NC_012779.2.

3D structure databases

ProteinModelPortaliC5BC46.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi634503.NT01EI_3833.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACR70952; ACR70952; NT01EI_3833.
GeneIDi7959874.
KEGGieic:NT01EI_3833.
PATRICi21840359. VBIEdwIct114273_3420.

Phylogenomic databases

eggNOGiENOG4105F0S. Bacteria.
COG3700. LUCA.
HOGENOMiHOG000270623.
KOiK03788.
OMAiPEFWEKM.
OrthoDBiPOG091H06BK.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR005519. Acid_phosphat_B-like.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamiPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01672. AphA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAPHA_EDWI9
AccessioniPrimary (citable) accession number: C5BC46
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: July 28, 2009
Last modified: November 2, 2016
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.