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C5BC46 (APHA_EDWI9) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 30. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Class B acid phosphatase

Short name=CBAP
EC=3.1.3.2
Gene names
Name:aphA
Ordered Locus Names:NT01EI_3833
OrganismEdwardsiella ictaluri (strain 93-146) [Complete proteome] [HAMAP]
Taxonomic identifier634503 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEdwardsiella

Protein attributes

Sequence length241 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Dephosphorylates several organic phosphate monoesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds By similarity.

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Subunit structure

Homotetramer By similarity.

Subcellular location

Periplasm By similarity.

Sequence similarities

Belongs to the class B bacterial acid phosphatase family.

Ontologies

Keywords
   Cellular componentPeriplasm
   DomainSignal
   LigandMagnesium
Metal-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentouter membrane-bounded periplasmic space

Inferred from electronic annotation. Source: InterPro

   Molecular_functionacid phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Chain28 – 241214Class B acid phosphatase
PRO_0000415225

Regions

Region141 – 1422Substrate binding By similarity

Sites

Active site721Nucleophile By similarity
Active site741Proton donor By similarity
Metal binding721Magnesium By similarity
Metal binding741Magnesium; via carbonyl oxygen By similarity
Metal binding1961Magnesium By similarity
Binding site1811Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
C5BC46 [UniParc].

Last modified July 28, 2009. Version 1.
Checksum: 1BD08167680BA319

FASTA24126,760
        10         20         30         40         50         60 
MFITTKKSLI ALVLATAGLI SSPVSFATET GAQPNLGATL TQLTQQYPIH WISIEQVAES 

        70         80         90        100        110        120 
LKGKAPISVG FDIDDTLLFS SPAFFYGKQK FSPDSNAFLK NQKFWDAVSS SGWDRFSIPK 

       130        140        150        160        170        180 
DSGRALMELH LKRGDHVYFI TGRPMPSNGK EDLTQTLKDD FKIPDNQLNK VIFAGTKQDA 

       190        200        210        220        230        240 
KVEYMQKYHI TIFYGDSDND IQDARKAGAE GIRVLRPLNS TNKPMPKNGA FGEKVIVNSQ 


Y 

« Hide

References

[1]"Complete genome sequence of Edwardsiella ictaluri 93-146."
Williams M.L., Gillaspy A.F., Dyer D.W., Thune R.L., Waldbieser G.C., Schuster S.C., Gipson J., Zaitshik J., Landry C., Lawrence M.L.
Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 93-146.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001600 Genomic DNA. Translation: ACR70952.1.
RefSeqYP_002935187.1. NC_012779.2.

3D structure databases

ProteinModelPortalC5BC46.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING634503.NT01EI_3833.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACR70952; ACR70952; NT01EI_3833.
GeneID7959874.
KEGGeic:NT01EI_3833.
PATRIC21840359. VBIEdwIct114273_3420.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3700.
HOGENOMHOG000270623.
KOK03788.
OMAPEFWEKM.
OrthoDBEOG6HTNVZ.
ProtClustDBPRK11009.

Enzyme and pathway databases

BioCycEICT634503:GCMY-3809-MONOMER.

Family and domain databases

Gene3D3.40.50.1000. 1 hit.
InterProIPR005519. Acid_phosphat_B.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
PIRSFPIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMSSF56784. SSF56784. 1 hit.
TIGRFAMsTIGR01672. AphA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameAPHA_EDWI9
AccessionPrimary (citable) accession number: C5BC46
Entry history
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: July 28, 2009
Last modified: April 16, 2014
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families