Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

C5BC46

- APHA_EDWI9

UniProt

C5BC46 - APHA_EDWI9

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Class B acid phosphatase

Gene

aphA

Organism
Edwardsiella ictaluri (strain 93-146)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Dephosphorylates several organic phosphate monoesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds (By similarity).By similarity

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.By similarity

Cofactori

Mg2+By similarityNote: Binds 1 Mg(2+) ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei72 – 721NucleophileBy similarity
Metal bindingi72 – 721MagnesiumBy similarity
Active sitei74 – 741Proton donorBy similarity
Metal bindingi74 – 741Magnesium; via carbonyl oxygenBy similarity
Binding sitei181 – 1811SubstrateBy similarity
Metal bindingi196 – 1961MagnesiumBy similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciEICT634503:GCMY-3809-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Class B acid phosphataseBy similarity (EC:3.1.3.2By similarity)
Short name:
CBAPBy similarity
Gene namesi
Name:aphABy similarity
Ordered Locus Names:NT01EI_3833
OrganismiEdwardsiella ictaluri (strain 93-146)
Taxonomic identifieri634503 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEdwardsiella
ProteomesiUP000001485: Chromosome

Subcellular locationi

Periplasm By similarity

GO - Cellular componenti

  1. outer membrane-bounded periplasmic space Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence AnalysisAdd
BLAST
Chaini28 – 241214Class B acid phosphataseSequence AnalysisPRO_0000415225Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi634503.NT01EI_3833.

Structurei

3D structure databases

ProteinModelPortaliC5BC46.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni141 – 1422Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class B bacterial acid phosphatase family.By similarity

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3700.
HOGENOMiHOG000270623.
KOiK03788.
OMAiPEFWEKM.
OrthoDBiEOG6HTNVZ.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR005519. Acid_phosphat_B.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamiPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
PIRSFiPIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01672. AphA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

C5BC46-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFITTKKSLI ALVLATAGLI SSPVSFATET GAQPNLGATL TQLTQQYPIH
60 70 80 90 100
WISIEQVAES LKGKAPISVG FDIDDTLLFS SPAFFYGKQK FSPDSNAFLK
110 120 130 140 150
NQKFWDAVSS SGWDRFSIPK DSGRALMELH LKRGDHVYFI TGRPMPSNGK
160 170 180 190 200
EDLTQTLKDD FKIPDNQLNK VIFAGTKQDA KVEYMQKYHI TIFYGDSDND
210 220 230 240
IQDARKAGAE GIRVLRPLNS TNKPMPKNGA FGEKVIVNSQ Y
Length:241
Mass (Da):26,760
Last modified:July 28, 2009 - v1
Checksum:i1BD08167680BA319
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001600 Genomic DNA. Translation: ACR70952.1.
RefSeqiYP_002935187.1. NC_012779.2.

Genome annotation databases

EnsemblBacteriaiACR70952; ACR70952; NT01EI_3833.
GeneIDi7959874.
KEGGieic:NT01EI_3833.
PATRICi21840359. VBIEdwIct114273_3420.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001600 Genomic DNA. Translation: ACR70952.1 .
RefSeqi YP_002935187.1. NC_012779.2.

3D structure databases

ProteinModelPortali C5BC46.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 634503.NT01EI_3833.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACR70952 ; ACR70952 ; NT01EI_3833 .
GeneIDi 7959874.
KEGGi eic:NT01EI_3833.
PATRICi 21840359. VBIEdwIct114273_3420.

Phylogenomic databases

eggNOGi COG3700.
HOGENOMi HOG000270623.
KOi K03788.
OMAi PEFWEKM.
OrthoDBi EOG6HTNVZ.

Enzyme and pathway databases

BioCyci EICT634503:GCMY-3809-MONOMER.

Family and domain databases

Gene3Di 3.40.50.1000. 1 hit.
InterProi IPR005519. Acid_phosphat_B.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view ]
Pfami PF03767. Acid_phosphat_B. 1 hit.
[Graphical view ]
PIRSFi PIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMi SSF56784. SSF56784. 1 hit.
TIGRFAMsi TIGR01672. AphA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Edwardsiella ictaluri 93-146."
    Williams M.L., Gillaspy A.F., Dyer D.W., Thune R.L., Waldbieser G.C., Schuster S.C., Gipson J., Zaitshik J., Landry C., Lawrence M.L.
    Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 93-1461 Publication.

Entry informationi

Entry nameiAPHA_EDWI9
AccessioniPrimary (citable) accession number: C5BC46
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: July 28, 2009
Last modified: November 26, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3