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C5BC26

- PFKA_EDWI9

UniProt

C5BC26 - PFKA_EDWI9

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Protein

ATP-dependent 6-phosphofructokinase

Gene
pfkA, NT01EI_3808
Organism
Edwardsiella ictaluri (strain 93-146)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei12 – 121ATP; via amide nitrogen By similarity
Metal bindingi104 – 1041Magnesium; catalytic By similarity
Active sitei128 – 1281Proton acceptor By similarity
Binding sitei155 – 1551Allosteric activator ADP By similarity
Binding sitei163 – 1631Substrate; shared with dimeric partner By similarity
Binding sitei212 – 2121Allosteric activator ADP By similarity
Binding sitei223 – 2231Substrate By similarity
Binding sitei244 – 2441Substrate; shared with dimeric partner By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi73 – 742ATP By similarity
Nucleotide bindingi103 – 1064ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEICT634503:GCMY-3782-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase (EC:2.7.1.11)
Short name:
ATP-PFK
Short name:
Phosphofructokinase
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:pfkA
Ordered Locus Names:NT01EI_3808
OrganismiEdwardsiella ictaluri (strain 93-146)
Taxonomic identifieri634503 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEdwardsiella
ProteomesiUP000001485: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 320320ATP-dependent 6-phosphofructokinaseUniRule annotationPRO_1000205246Add
BLAST

Proteomic databases

PRIDEiC5BC26.

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi634503.NT01EI_3808.

Structurei

3D structure databases

ProteinModelPortaliC5BC26.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni22 – 265Allosteric activator ADP binding; shared with dimeric partner By similarity
Regioni126 – 1283Substrate binding By similarity
Regioni170 – 1723Substrate binding By similarity
Regioni186 – 1883Allosteric activator ADP binding By similarity
Regioni214 – 2163Allosteric activator ADP binding By similarity
Regioni250 – 2534Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248870.
KOiK00850.
OMAiGFGGRCV.
OrthoDBiEOG644ZRM.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1.
InterProiIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C5BC26-1 [UniParc]FASTAAdd to Basket

« Hide

MIKKIGVLTS GGDAPGMNAA IRGVVRAALS KGLDVYGIHD GYLGLYEGRI    50
EQLDRHSVSD VINRGGTFLG SARFPEFRDE KVREKAVENM REFGLDALVV 100
IGGDGSYLGA KRLTEMGFPC IGLPGTIDND VAGTDYTIGY FTALETVVEA 150
IDRLRDTSSS HQRISIVEVM GRYCGDLTLA AAIAGGCEYI VLPEVEFKRE 200
DLVEEIKVGI TKGKKHAIVA ITEHICDVDE LAKFIEQETG RETRSTVLGH 250
IQRGGAPVAY DRILASRMGA YSIELLLQGY GGRCVGIQNE KLVHHDIIDA 300
VENMKRPFKG DWLDTAKMLF 320
Length:320
Mass (Da):34,953
Last modified:July 28, 2009 - v1
Checksum:i043646A6A394FCD1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001600 Genomic DNA. Translation: ACR70932.1.
RefSeqiWP_015872965.1. NC_012779.2.
YP_002935167.1. NC_012779.2.

Genome annotation databases

EnsemblBacteriaiACR70932; ACR70932; NT01EI_3808.
GeneIDi7959316.
KEGGieic:NT01EI_3808.
PATRICi21840309. VBIEdwIct114273_3401.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001600 Genomic DNA. Translation: ACR70932.1 .
RefSeqi WP_015872965.1. NC_012779.2.
YP_002935167.1. NC_012779.2.

3D structure databases

ProteinModelPortali C5BC26.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 634503.NT01EI_3808.

Proteomic databases

PRIDEi C5BC26.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACR70932 ; ACR70932 ; NT01EI_3808 .
GeneIDi 7959316.
KEGGi eic:NT01EI_3808.
PATRICi 21840309. VBIEdwIct114273_3401.

Phylogenomic databases

eggNOGi COG0205.
HOGENOMi HOG000248870.
KOi K00850.
OMAi GFGGRCV.
OrthoDBi EOG644ZRM.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci EICT634503:GCMY-3782-MONOMER.

Family and domain databases

HAMAPi MF_00339. Phosphofructokinase_I_B1.
InterProi IPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Edwardsiella ictaluri 93-146."
    Williams M.L., Gillaspy A.F., Dyer D.W., Thune R.L., Waldbieser G.C., Schuster S.C., Gipson J., Zaitshik J., Landry C., Lawrence M.L.
    Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 93-146.

Entry informationi

Entry nameiPFKA_EDWI9
AccessioniPrimary (citable) accession number: C5BC26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: September 3, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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