C5B8I4 (NUOCD_EDWI9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit C/D
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit C/D
NDH-1 subunit C/D

Gene names

Name:	nuoC
Synonyms:	nuoCD, nuoD
Ordered Locus Names:	NT01EI_2677

Organism

Edwardsiella ictaluri (strain 93-146) [Complete proteome] [HAMAP]

Taxonomic identifier

634503 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Edwardsiella › Edwardsiella ictaluri

Protein attributes

Sequence length	598 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP-Rule MF_01359
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP-Rule MF_01359
Subunit structure	NDH-1 is composed of 13 different subunits. Subunits NuoB, CD, E, F, and G constitute the peripheral sector of the complex By similarity.
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP-Rule MF_01359.
Sequence similarities	In the N-terminal section; belongs to the complex I 30 kDa subunit family. In the C-terminal section; belongs to the complex I 49 kDa subunit family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological_process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	NADH dehydrogenase complex Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	NAD binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 598	598	NADH-quinone oxidoreductase subunit C/D HAMAP-Rule MF_01359		PRO_1000214870
Regions
	Region	1 – 189	189	NADH dehydrogenase I subunit C By similarity
	Region	213 – 598	386	NADH dehydrogenase I subunit D By similarity

Sequences

	Sequence		Length	Mass (Da)
	C5B8I4 [UniParc]. Last modified July 28, 2009. Version 1. Checksum: 117C5648EB6E95AD	FASTA	598	68,866
	10 20 30 40 50 60 MTDSTTHDRE MSAWQTHDHL DDPVIGELRN RFGPEAFSVQ PTRTGMPVVW VKREQLLEVM 70 80 90 100 110 120 AFLRQLPKPY VMLFDLHGMD ERLRTHRQGL PAADFSVFYH LISIDRNRDI MLKVALAEQD 130 140 150 160 170 180 LHLPTVTRLF PNANWYERET FDLFGITFDG HPHLTRLLMP RTWQGHPLRK DYPARATEFD 190 200 210 220 230 240 PFELTRQKQD LEMESLKFRP EEWGMQRGTA NEDFMFLNLG PNHPSSHGAF RIVLQLDGED 250 260 270 280 290 300 IVDCVPDIGY HHRGAEKMGE RQSWHSYIPY TDRIEYLGGC VNEMPYVLAV EKLAGIEVPE 310 320 330 340 350 360 RVKVIRVMLS ELFRINSHLL YISTFIQDVG AMTPVFFAFT DRQKIYDIVE AVTGFRMHPA 370 380 390 400 410 420 WFRIGGVAHD LPKGWDRLLR EFLEWMPKRL DSYVKAALHN SILKGRSIGV AAYGAKDALA 430 440 450 460 470 480 WGTTGAGLRA TGIAFDVRKW RPYSGYENFE FEVPTASNGD CYDRVMLKVE EIRQSLRILQ 490 500 510 520 530 540 QCLKNMPEGP FKADHPLTTP PPKERTLQHI DTLINHFLQV SWGPVMPANE SFQMVEATKG 550 560 570 580 590 INSYYLTSDG GTMSYRTRIR TPSYAHLQQI PSVIRGCLVS DLIVYLGSID FVMSDVDR

References

[1]

"Complete genome sequence of Edwardsiella ictaluri 93-146." Williams M.L., Gillaspy A.F., Dyer D.W., Thune R.L., Waldbieser G.C., Schuster S.C., Gipson J., Zaitshik J., Landry C., Lawrence M.L. Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 93-146.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001600 Genomic DNA. Translation: ACR69845.1.
RefSeq	YP_002934080.1. NC_012779.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	634503.NT01EI_2677.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACR69845; ACR69845; NT01EI_2677.
GeneID	7961703.
KEGG	eic:NT01EI_2677.
PATRIC	21838214. VBIEdwIct114273_2392.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0649.
HOGENOM	HOG000228264.
KO	K13378.
OMA	VWDMFGI.
ProtClustDB	PRK11742.
Family and domain databases
HAMAP	MF_01359. NDH1_NuoCD_1.
InterPro	IPR010218. NADH_DH_suC. IPR023062. NADH_DH_suCD. IPR001135. NADH_Q_OxRdtase_suD. IPR001268. NADH_UbQ_OxRdtase_30kDa_su. IPR014029. NADH_UbQ_OxRdtase_49kDa_CS. IPR022885. NDH1_su_D/H. [Graphical view]
Pfam	PF00329. Complex1_30kDa. 1 hit. PF00346. Complex1_49kDa. 1 hit. [Graphical view]
ProDom	PD001581. NADH_UbQ_OxRdtase_30kDa_su. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01961. NuoC_fam. 1 hit. TIGR01962. NuoD. 1 hit.
PROSITE	PS00542. COMPLEX1_30K. False negative. PS00535. COMPLEX1_49K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOCD_EDWI9

Accession

Primary (citable) accession number: C5B8I4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	July 28, 2009
Last modified:	May 1, 2013
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program