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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Edwardsiella ictaluri (strain 93-146)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei572Aminoacyl-adenylateUniRule annotation1
Binding sitei616ATPUniRule annotation1
Metal bindingi911ZincUniRule annotation1
Metal bindingi914ZincUniRule annotation1
Metal bindingi931ZincUniRule annotation1
Metal bindingi934ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciEICT634503:G1GVC-630-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:NT01EI_0679
OrganismiEdwardsiella ictaluri (strain 93-146)
Taxonomic identifieri634503 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesHafniaceaeEdwardsiella
Proteomesi
  • UP000001485 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002162351 – 948Isoleucine--tRNA ligaseAdd BLAST948

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi634503.NT01EI_0679

Structurei

3D structure databases

ProteinModelPortaliC5B7M4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi58 – 68"HIGH" regionAdd BLAST11
Motifi613 – 617"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.6202 hits
3.90.740.101 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

C5B7M4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDYKNTLNL PETGFPMRGD LAKREPGMLQ HWYEQDLYGI IRNAKQGKKS
60 70 80 90 100
FILHDGPPYA NGSIHIGHSV NKILKDIIIK SKGLSGYDSP YIPGWDCHGL
110 120 130 140 150
PIELKVEQLI GKPGEKYSAA EFREQCRAYA AEQVAGQKAD FIRLGVLGDW
160 170 180 190 200
EHPYLTMDYG TEANIIRALA RIVDNGHLMK GAKPVHWCPD CGSSLAEAEV
210 220 230 240 250
EYYDKVSPSI DVRFAAVDKA QVLAKFGVSE AKGDVNVVIW TTTPWTLPAN
260 270 280 290 300
RAVALHPELE YQLVQVEGEC LILAADLVES VMKRAGIDYW QVLGSCKGEA
310 320 330 340 350
LELLHFCHPF MNFDVPIIMG DHVTLDAGTG AVHTAPGHGP DDYVVGQKYG
360 370 380 390 400
LEIANPVGSN GCYLPGTHPV LDGLFVFKAN DIVIDLLKDS GALLHVEKLT
410 420 430 440 450
HSYPCCWRHK SPIIFRATPQ WFISMDRQGL RSQSLAEIDR IERQGLDEQN
460 470 480 490 500
LSGWIPAWGK ARIESMVANR PDWCISRQRT WGVPMAMLVH KETQELHPRT
510 520 530 540 550
TELMEMVAKR VEQAGIQAWW DLDIRDLLGD EADQYEKVPD TLDVWFDSGS
560 570 580 590 600
TSYSVVDARP EFNGHSPDMY LEGSDQHRGW FMSSLMISVA MKGKAPYRQV
610 620 630 640 650
LTHGFTVDGQ GRKMSKSVGN VVSPQQVMNK LGGDILRLWV ASTDYTSEMA
660 670 680 690 700
VSDEILKRSA DAYRRIRNTA RFLLANLNGF DPCKDMVKPE DMVVLDRWAV
710 720 730 740 750
GCAKQAQDEI IAAYENYDFH EVVQRLMQFC SVEMGSFYLD IIKDRQYTAK
760 770 780 790 800
ADSVARRSCQ TALYHIAEAL VRWMAPILSF TADEVWGYLP GDRAQFVFTE
810 820 830 840 850
EWYQGLFGLD AAEQMNDAFW AELLKVRGEV NRVIEQARND KKVGGSLEAA
860 870 880 890 900
ITLYADEALA TQLDSLQDEL RFVLITSAAR VQPLVQATAD AVASELAGLK
910 920 930 940
VGLGKADGSK CPRCWHYSTA IGQDAAHPQL CPRCVTNVAG QGEERKFA
Length:948
Mass (Da):105,791
Last modified:July 28, 2009 - v1
Checksum:i2399B079470C2A0C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001600 Genomic DNA Translation: ACR67901.1
RefSeqiWP_015870094.1, NC_012779.2

Genome annotation databases

EnsemblBacteriaiACR67901; ACR67901; NT01EI_0679
GeneIDi7962013
KEGGieic:NT01EI_0679
PATRICifig|634503.3.peg.611

Similar proteinsi

Entry informationi

Entry nameiSYI_EDWI9
AccessioniPrimary (citable) accession number: C5B7M4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: March 28, 2018
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome