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C5B7M4

- SYI_EDWI9

UniProt

C5B7M4 - SYI_EDWI9

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Edwardsiella ictaluri (strain 93-146)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei572 – 5721Aminoacyl-adenylateUniRule annotation
Binding sitei616 – 6161ATPUniRule annotation
Metal bindingi911 – 9111ZincUniRule annotation
Metal bindingi914 – 9141ZincUniRule annotation
Metal bindingi931 – 9311ZincUniRule annotation
Metal bindingi934 – 9341ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciEICT634503:GCMY-674-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:NT01EI_0679
OrganismiEdwardsiella ictaluri (strain 93-146)
Taxonomic identifieri634503 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEdwardsiella
ProteomesiUP000001485: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 948948Isoleucine--tRNA ligasePRO_1000216235Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi634503.NT01EI_0679.

Structurei

3D structure databases

ProteinModelPortaliC5B7M4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi613 – 6175"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C5B7M4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDYKNTLNL PETGFPMRGD LAKREPGMLQ HWYEQDLYGI IRNAKQGKKS
60 70 80 90 100
FILHDGPPYA NGSIHIGHSV NKILKDIIIK SKGLSGYDSP YIPGWDCHGL
110 120 130 140 150
PIELKVEQLI GKPGEKYSAA EFREQCRAYA AEQVAGQKAD FIRLGVLGDW
160 170 180 190 200
EHPYLTMDYG TEANIIRALA RIVDNGHLMK GAKPVHWCPD CGSSLAEAEV
210 220 230 240 250
EYYDKVSPSI DVRFAAVDKA QVLAKFGVSE AKGDVNVVIW TTTPWTLPAN
260 270 280 290 300
RAVALHPELE YQLVQVEGEC LILAADLVES VMKRAGIDYW QVLGSCKGEA
310 320 330 340 350
LELLHFCHPF MNFDVPIIMG DHVTLDAGTG AVHTAPGHGP DDYVVGQKYG
360 370 380 390 400
LEIANPVGSN GCYLPGTHPV LDGLFVFKAN DIVIDLLKDS GALLHVEKLT
410 420 430 440 450
HSYPCCWRHK SPIIFRATPQ WFISMDRQGL RSQSLAEIDR IERQGLDEQN
460 470 480 490 500
LSGWIPAWGK ARIESMVANR PDWCISRQRT WGVPMAMLVH KETQELHPRT
510 520 530 540 550
TELMEMVAKR VEQAGIQAWW DLDIRDLLGD EADQYEKVPD TLDVWFDSGS
560 570 580 590 600
TSYSVVDARP EFNGHSPDMY LEGSDQHRGW FMSSLMISVA MKGKAPYRQV
610 620 630 640 650
LTHGFTVDGQ GRKMSKSVGN VVSPQQVMNK LGGDILRLWV ASTDYTSEMA
660 670 680 690 700
VSDEILKRSA DAYRRIRNTA RFLLANLNGF DPCKDMVKPE DMVVLDRWAV
710 720 730 740 750
GCAKQAQDEI IAAYENYDFH EVVQRLMQFC SVEMGSFYLD IIKDRQYTAK
760 770 780 790 800
ADSVARRSCQ TALYHIAEAL VRWMAPILSF TADEVWGYLP GDRAQFVFTE
810 820 830 840 850
EWYQGLFGLD AAEQMNDAFW AELLKVRGEV NRVIEQARND KKVGGSLEAA
860 870 880 890 900
ITLYADEALA TQLDSLQDEL RFVLITSAAR VQPLVQATAD AVASELAGLK
910 920 930 940
VGLGKADGSK CPRCWHYSTA IGQDAAHPQL CPRCVTNVAG QGEERKFA
Length:948
Mass (Da):105,791
Last modified:July 28, 2009 - v1
Checksum:i2399B079470C2A0C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001600 Genomic DNA. Translation: ACR67901.1.
RefSeqiWP_015870094.1. NC_012779.2.
YP_002932136.1. NC_012779.2.

Genome annotation databases

EnsemblBacteriaiACR67901; ACR67901; NT01EI_0679.
GeneIDi7962013.
KEGGieic:NT01EI_0679.
PATRICi21834582. VBIEdwIct114273_0611.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001600 Genomic DNA. Translation: ACR67901.1 .
RefSeqi WP_015870094.1. NC_012779.2.
YP_002932136.1. NC_012779.2.

3D structure databases

ProteinModelPortali C5B7M4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 634503.NT01EI_0679.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACR67901 ; ACR67901 ; NT01EI_0679 .
GeneIDi 7962013.
KEGGi eic:NT01EI_0679.
PATRICi 21834582. VBIEdwIct114273_0611.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci EICT634503:GCMY-674-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Edwardsiella ictaluri 93-146."
    Williams M.L., Gillaspy A.F., Dyer D.W., Thune R.L., Waldbieser G.C., Schuster S.C., Gipson J., Zaitshik J., Landry C., Lawrence M.L.
    Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 93-146.

Entry informationi

Entry nameiSYI_EDWI9
AccessioniPrimary (citable) accession number: C5B7M4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: November 26, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3