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C5B7M4

- SYI_EDWI9

UniProt

C5B7M4 - SYI_EDWI9

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Protein
Isoleucine--tRNA ligase
Gene
ileS, NT01EI_0679
Organism
Edwardsiella ictaluri (strain 93-146)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei572 – 5721Aminoacyl-adenylate By similarity
Binding sitei616 – 6161ATP By similarity
Metal bindingi911 – 9111Zinc By similarity
Metal bindingi914 – 9141Zinc By similarity
Metal bindingi931 – 9311Zinc By similarity
Metal bindingi934 – 9341Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciEICT634503:GCMY-674-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:NT01EI_0679
OrganismiEdwardsiella ictaluri (strain 93-146)
Taxonomic identifieri634503 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEdwardsiella
ProteomesiUP000001485: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 948948Isoleucine--tRNA ligaseUniRule annotation
PRO_1000216235Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi634503.NT01EI_0679.

Structurei

3D structure databases

ProteinModelPortaliC5B7M4.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotation
Add
BLAST
Motifi613 – 6175"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C5B7M4-1 [UniParc]FASTAAdd to Basket

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MSDYKNTLNL PETGFPMRGD LAKREPGMLQ HWYEQDLYGI IRNAKQGKKS    50
FILHDGPPYA NGSIHIGHSV NKILKDIIIK SKGLSGYDSP YIPGWDCHGL 100
PIELKVEQLI GKPGEKYSAA EFREQCRAYA AEQVAGQKAD FIRLGVLGDW 150
EHPYLTMDYG TEANIIRALA RIVDNGHLMK GAKPVHWCPD CGSSLAEAEV 200
EYYDKVSPSI DVRFAAVDKA QVLAKFGVSE AKGDVNVVIW TTTPWTLPAN 250
RAVALHPELE YQLVQVEGEC LILAADLVES VMKRAGIDYW QVLGSCKGEA 300
LELLHFCHPF MNFDVPIIMG DHVTLDAGTG AVHTAPGHGP DDYVVGQKYG 350
LEIANPVGSN GCYLPGTHPV LDGLFVFKAN DIVIDLLKDS GALLHVEKLT 400
HSYPCCWRHK SPIIFRATPQ WFISMDRQGL RSQSLAEIDR IERQGLDEQN 450
LSGWIPAWGK ARIESMVANR PDWCISRQRT WGVPMAMLVH KETQELHPRT 500
TELMEMVAKR VEQAGIQAWW DLDIRDLLGD EADQYEKVPD TLDVWFDSGS 550
TSYSVVDARP EFNGHSPDMY LEGSDQHRGW FMSSLMISVA MKGKAPYRQV 600
LTHGFTVDGQ GRKMSKSVGN VVSPQQVMNK LGGDILRLWV ASTDYTSEMA 650
VSDEILKRSA DAYRRIRNTA RFLLANLNGF DPCKDMVKPE DMVVLDRWAV 700
GCAKQAQDEI IAAYENYDFH EVVQRLMQFC SVEMGSFYLD IIKDRQYTAK 750
ADSVARRSCQ TALYHIAEAL VRWMAPILSF TADEVWGYLP GDRAQFVFTE 800
EWYQGLFGLD AAEQMNDAFW AELLKVRGEV NRVIEQARND KKVGGSLEAA 850
ITLYADEALA TQLDSLQDEL RFVLITSAAR VQPLVQATAD AVASELAGLK 900
VGLGKADGSK CPRCWHYSTA IGQDAAHPQL CPRCVTNVAG QGEERKFA 948
Length:948
Mass (Da):105,791
Last modified:July 28, 2009 - v1
Checksum:i2399B079470C2A0C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001600 Genomic DNA. Translation: ACR67901.1.
RefSeqiYP_002932136.1. NC_012779.2.

Genome annotation databases

EnsemblBacteriaiACR67901; ACR67901; NT01EI_0679.
GeneIDi7962013.
KEGGieic:NT01EI_0679.
PATRICi21834582. VBIEdwIct114273_0611.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001600 Genomic DNA. Translation: ACR67901.1 .
RefSeqi YP_002932136.1. NC_012779.2.

3D structure databases

ProteinModelPortali C5B7M4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 634503.NT01EI_0679.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACR67901 ; ACR67901 ; NT01EI_0679 .
GeneIDi 7962013.
KEGGi eic:NT01EI_0679.
PATRICi 21834582. VBIEdwIct114273_0611.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci EICT634503:GCMY-674-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Edwardsiella ictaluri 93-146."
    Williams M.L., Gillaspy A.F., Dyer D.W., Thune R.L., Waldbieser G.C., Schuster S.C., Gipson J., Zaitshik J., Landry C., Lawrence M.L.
    Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 93-146.

Entry informationi

Entry nameiSYI_EDWI9
AccessioniPrimary (citable) accession number: C5B7M4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: May 14, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi