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C5B7B9

- NAPA_EDWI9

UniProt

C5B7B9 - NAPA_EDWI9

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Protein
Periplasmic nitrate reductase
Gene
napA, NT01EI_1217
Organism
Edwardsiella ictaluri (strain 93-146)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster By similarity.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471Iron-sulfur (4Fe-4S) By similarity
Metal bindingi50 – 501Iron-sulfur (4Fe-4S) By similarity
Metal bindingi54 – 541Iron-sulfur (4Fe-4S) By similarity
Metal bindingi82 – 821Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciEICT634503:GCMY-1202-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductase (EC:1.7.99.4)
Gene namesi
Name:napA
Ordered Locus Names:NT01EI_1217
OrganismiEdwardsiella ictaluri (strain 93-146)
Taxonomic identifieri634503 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEdwardsiella
ProteomesiUP000001485: Chromosome

Subcellular locationi

Periplasm By similarity UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Tat-type signal Reviewed prediction
Add
BLAST
Chaini32 – 829798Periplasmic nitrate reductaseUniRule annotation
PRO_1000215768Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Interacts with NapB By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi634503.NT01EI_1217.

Structurei

3D structure databases

ProteinModelPortaliC5B7B9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 96574Fe-4S Mo/W bis-MGD-type
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiAGKMHDS.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

C5B7B9-1 [UniParc]FASTAAdd to Basket

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MKLSRRDFMK ANAVAAAAAA AGLTIPTVAR AVTDAGSDGI TWDKAPCRFC    50
GTGCGVLVGT QNGRIVASQG DPDAPVNRGL NCIKGYFLPK IMYGKDRLTQ 100
PLLRMKGGQY DKEGEFTPVS WDQAFDVMAA KFKGTLKEKG PGAVGMFGSG 150
QWTVWEGYAA AKLFKAGFRS NNLDPNARHC MASAVVGFMR TFGMDEPMGC 200
YDDIEQADAF VLWGSNMAEM HPILWSRITN RRLSNDKVQV AVLSTFEQRS 250
FELADNPIIF TPQSDLVILN YIANYIIQNN AVDQAFMDKH VNIRKGATDI 300
GYGLRPTDPL EKAAKNPGSD ASEPMSFDDY KAFVADYTLE KTAKMTGVPQ 350
DQLLALAKLY ADPNIKVVSY WTMGFNQHTR GVWANNLCYN IHLLTGKISK 400
PGCGPFSLTG QPSACGTARE VGTFAHRLPA DMVVTNEKHR QIAETRWKIP 450
AGTIPDKVGL HAVAQDRALK DGKLNAYWVM CNNNMQAGPN INADRMPGWR 500
DPRNFIVVSD PYPTVSALSA DLILPTAMWV EKEGAYGNAE RRTQFWHQQV 550
KAPGEAKSDL WQLMEFSKRF TVDEVWPAAL LAKMPALQGK TLYEVLYVNG 600
NVDRYPLDEV PADRLNDEAR ACGFYVQKGL FEEYAGFGRG HGHDLAPFDA 650
YHKARGIRWP VVDGKETLWR YREGYDPFVK NGEEVRFYGK PDGKAVIFAL 700
PYEPPAESPD QEYDLWLSTG RVLEHWHTGS MTRRVPELHR AFPEAVLFIH 750
PLDAKARGLR RGDKVKVMSR RGELVSIVET RGRNRVPQGL VYMPFFDAAQ 800
LVNVLTLDAT DPLSKEADFK KCAVKIEKV 829
Length:829
Mass (Da):92,241
Last modified:July 28, 2009 - v1
Checksum:iBB81F67C978D1141
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001600 Genomic DNA. Translation: ACR68425.1.
RefSeqiYP_002932660.1. NC_012779.2.

Genome annotation databases

EnsemblBacteriaiACR68425; ACR68425; NT01EI_1217.
GeneIDi7960963.
KEGGieic:NT01EI_1217.
PATRICi21835580. VBIEdwIct114273_1106.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001600 Genomic DNA. Translation: ACR68425.1 .
RefSeqi YP_002932660.1. NC_012779.2.

3D structure databases

ProteinModelPortali C5B7B9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 634503.NT01EI_1217.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACR68425 ; ACR68425 ; NT01EI_1217 .
GeneIDi 7960963.
KEGGi eic:NT01EI_1217.
PATRICi 21835580. VBIEdwIct114273_1106.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi AGKMHDS.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci EICT634503:GCMY-1202-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Edwardsiella ictaluri 93-146."
    Williams M.L., Gillaspy A.F., Dyer D.W., Thune R.L., Waldbieser G.C., Schuster S.C., Gipson J., Zaitshik J., Landry C., Lawrence M.L.
    Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 93-146.

Entry informationi

Entry nameiNAPA_EDWI9
AccessioniPrimary (citable) accession number: C5B7B9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: May 14, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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