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C5B7B9

- NAPA_EDWI9

UniProt

C5B7B9 - NAPA_EDWI9

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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Edwardsiella ictaluri (strain 93-146)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi50 – 501Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi54 – 541Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi82 – 821Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciEICT634503:GCMY-1202-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:NT01EI_1217
OrganismiEdwardsiella ictaluri (strain 93-146)
Taxonomic identifieri634503 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEdwardsiella
ProteomesiUP000001485: Chromosome

Subcellular locationi

Periplasm UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Tat-type signalUniRule annotationAdd
BLAST
Chaini32 – 829798Periplasmic nitrate reductasePRO_1000215768Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi634503.NT01EI_1217.

Structurei

3D structure databases

ProteinModelPortaliC5B7B9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 96574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiAGKMHDS.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

C5B7B9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLSRRDFMK ANAVAAAAAA AGLTIPTVAR AVTDAGSDGI TWDKAPCRFC
60 70 80 90 100
GTGCGVLVGT QNGRIVASQG DPDAPVNRGL NCIKGYFLPK IMYGKDRLTQ
110 120 130 140 150
PLLRMKGGQY DKEGEFTPVS WDQAFDVMAA KFKGTLKEKG PGAVGMFGSG
160 170 180 190 200
QWTVWEGYAA AKLFKAGFRS NNLDPNARHC MASAVVGFMR TFGMDEPMGC
210 220 230 240 250
YDDIEQADAF VLWGSNMAEM HPILWSRITN RRLSNDKVQV AVLSTFEQRS
260 270 280 290 300
FELADNPIIF TPQSDLVILN YIANYIIQNN AVDQAFMDKH VNIRKGATDI
310 320 330 340 350
GYGLRPTDPL EKAAKNPGSD ASEPMSFDDY KAFVADYTLE KTAKMTGVPQ
360 370 380 390 400
DQLLALAKLY ADPNIKVVSY WTMGFNQHTR GVWANNLCYN IHLLTGKISK
410 420 430 440 450
PGCGPFSLTG QPSACGTARE VGTFAHRLPA DMVVTNEKHR QIAETRWKIP
460 470 480 490 500
AGTIPDKVGL HAVAQDRALK DGKLNAYWVM CNNNMQAGPN INADRMPGWR
510 520 530 540 550
DPRNFIVVSD PYPTVSALSA DLILPTAMWV EKEGAYGNAE RRTQFWHQQV
560 570 580 590 600
KAPGEAKSDL WQLMEFSKRF TVDEVWPAAL LAKMPALQGK TLYEVLYVNG
610 620 630 640 650
NVDRYPLDEV PADRLNDEAR ACGFYVQKGL FEEYAGFGRG HGHDLAPFDA
660 670 680 690 700
YHKARGIRWP VVDGKETLWR YREGYDPFVK NGEEVRFYGK PDGKAVIFAL
710 720 730 740 750
PYEPPAESPD QEYDLWLSTG RVLEHWHTGS MTRRVPELHR AFPEAVLFIH
760 770 780 790 800
PLDAKARGLR RGDKVKVMSR RGELVSIVET RGRNRVPQGL VYMPFFDAAQ
810 820
LVNVLTLDAT DPLSKEADFK KCAVKIEKV
Length:829
Mass (Da):92,241
Last modified:July 28, 2009 - v1
Checksum:iBB81F67C978D1141
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001600 Genomic DNA. Translation: ACR68425.1.
RefSeqiYP_002932660.1. NC_012779.2.

Genome annotation databases

EnsemblBacteriaiACR68425; ACR68425; NT01EI_1217.
GeneIDi7960963.
KEGGieic:NT01EI_1217.
PATRICi21835580. VBIEdwIct114273_1106.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001600 Genomic DNA. Translation: ACR68425.1 .
RefSeqi YP_002932660.1. NC_012779.2.

3D structure databases

ProteinModelPortali C5B7B9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 634503.NT01EI_1217.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACR68425 ; ACR68425 ; NT01EI_1217 .
GeneIDi 7960963.
KEGGi eic:NT01EI_1217.
PATRICi 21835580. VBIEdwIct114273_1106.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi AGKMHDS.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci EICT634503:GCMY-1202-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Edwardsiella ictaluri 93-146."
    Williams M.L., Gillaspy A.F., Dyer D.W., Thune R.L., Waldbieser G.C., Schuster S.C., Gipson J., Zaitshik J., Landry C., Lawrence M.L.
    Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 93-146.

Entry informationi

Entry nameiNAPA_EDWI9
AccessioniPrimary (citable) accession number: C5B7B9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: October 29, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3