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C5B7B9 (NAPA_EDWI9) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:NT01EI_1217
OrganismEdwardsiella ictaluri (strain 93-146) [Complete proteome] [HAMAP]
Taxonomic identifier634503 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEdwardsiella

Protein attributes

Sequence length829 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131Tat-type signal Potential
Chain32 – 829798Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_1000215768

Regions

Domain40 – 96574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding471Iron-sulfur (4Fe-4S) By similarity
Metal binding501Iron-sulfur (4Fe-4S) By similarity
Metal binding541Iron-sulfur (4Fe-4S) By similarity
Metal binding821Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
C5B7B9 [UniParc].

Last modified July 28, 2009. Version 1.
Checksum: BB81F67C978D1141

FASTA82992,241
        10         20         30         40         50         60 
MKLSRRDFMK ANAVAAAAAA AGLTIPTVAR AVTDAGSDGI TWDKAPCRFC GTGCGVLVGT 

        70         80         90        100        110        120 
QNGRIVASQG DPDAPVNRGL NCIKGYFLPK IMYGKDRLTQ PLLRMKGGQY DKEGEFTPVS 

       130        140        150        160        170        180 
WDQAFDVMAA KFKGTLKEKG PGAVGMFGSG QWTVWEGYAA AKLFKAGFRS NNLDPNARHC 

       190        200        210        220        230        240 
MASAVVGFMR TFGMDEPMGC YDDIEQADAF VLWGSNMAEM HPILWSRITN RRLSNDKVQV 

       250        260        270        280        290        300 
AVLSTFEQRS FELADNPIIF TPQSDLVILN YIANYIIQNN AVDQAFMDKH VNIRKGATDI 

       310        320        330        340        350        360 
GYGLRPTDPL EKAAKNPGSD ASEPMSFDDY KAFVADYTLE KTAKMTGVPQ DQLLALAKLY 

       370        380        390        400        410        420 
ADPNIKVVSY WTMGFNQHTR GVWANNLCYN IHLLTGKISK PGCGPFSLTG QPSACGTARE 

       430        440        450        460        470        480 
VGTFAHRLPA DMVVTNEKHR QIAETRWKIP AGTIPDKVGL HAVAQDRALK DGKLNAYWVM 

       490        500        510        520        530        540 
CNNNMQAGPN INADRMPGWR DPRNFIVVSD PYPTVSALSA DLILPTAMWV EKEGAYGNAE 

       550        560        570        580        590        600 
RRTQFWHQQV KAPGEAKSDL WQLMEFSKRF TVDEVWPAAL LAKMPALQGK TLYEVLYVNG 

       610        620        630        640        650        660 
NVDRYPLDEV PADRLNDEAR ACGFYVQKGL FEEYAGFGRG HGHDLAPFDA YHKARGIRWP 

       670        680        690        700        710        720 
VVDGKETLWR YREGYDPFVK NGEEVRFYGK PDGKAVIFAL PYEPPAESPD QEYDLWLSTG 

       730        740        750        760        770        780 
RVLEHWHTGS MTRRVPELHR AFPEAVLFIH PLDAKARGLR RGDKVKVMSR RGELVSIVET 

       790        800        810        820 
RGRNRVPQGL VYMPFFDAAQ LVNVLTLDAT DPLSKEADFK KCAVKIEKV 

« Hide

References

[1]"Complete genome sequence of Edwardsiella ictaluri 93-146."
Williams M.L., Gillaspy A.F., Dyer D.W., Thune R.L., Waldbieser G.C., Schuster S.C., Gipson J., Zaitshik J., Landry C., Lawrence M.L.
Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 93-146.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001600 Genomic DNA. Translation: ACR68425.1.
RefSeqYP_002932660.1. NC_012779.2.

3D structure databases

ProteinModelPortalC5B7B9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING634503.NT01EI_1217.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACR68425; ACR68425; NT01EI_1217.
GeneID7960963.
KEGGeic:NT01EI_1217.
PATRIC21835580. VBIEdwIct114273_1106.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMAFTTDEMW.
OrthoDBEOG6CVV7G.
ProtClustDBPRK13532.

Enzyme and pathway databases

BioCycEICT634503:GCMY-1202-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_EDWI9
AccessionPrimary (citable) accession number: C5B7B9
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: February 19, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families