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Protein

Glycerol-3-phosphate acyltransferase

Gene

plsB

Organism
Edwardsiella ictaluri (strain 93-146)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.UniRule annotation

Pathwayi: CDP-diacylglycerol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glycerol-3-phosphate acyltransferase (plsB)
  2. no protein annotated in this organism
  3. Phosphatidate cytidylyltransferase (NT01EI_0844)
This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciEICT634503:GCMY-224-MONOMER.
UniPathwayiUPA00557; UER00612.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate acyltransferaseUniRule annotation (EC:2.3.1.15UniRule annotation)
Short name:
GPATUniRule annotation
Gene namesi
Name:plsBUniRule annotation
Ordered Locus Names:NT01EI_0225
OrganismiEdwardsiella ictaluri (strain 93-146)
Taxonomic identifieri634503 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEdwardsiella
Proteomesi
  • UP000001485 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 818818Glycerol-3-phosphate acyltransferasePRO_1000205849Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi634503.NT01EI_0225.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi305 – 3106HXXXXD motif

Domaini

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.UniRule annotation

Sequence similaritiesi

Belongs to the GPAT/DAPAT family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E55. Bacteria.
COG2937. LUCA.
HOGENOMiHOG000218231.
KOiK00631.
OMAiEVIYVPC.
OrthoDBiEOG6B625B.

Family and domain databases

HAMAPiMF_00393. Glyc3P_acyltrans.
InterProiIPR022284. GPAT/DHAPAT.
IPR028354. GPAT_PlsB.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PANTHERiPTHR12563. PTHR12563. 2 hits.
PfamiPF01553. Acyltransferase. 1 hit.
[Graphical view]
PIRSFiPIRSF500064. GPAT. 1 hit.
PIRSF000437. GPAT_DHAPAT. 1 hit.
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03703. plsB. 1 hit.

Sequencei

Sequence statusi: Complete.

C5B706-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGWRKIYYK LLNLILKLLV KSKVIPTDPV AELRLDTTRP VFYVLPYNSK
60 70 80 90 100
VDLLTLRDRC LALDLPDPLD DNEIDGVILP RYVFIDDGPR VFRYYAPKQA
110 120 130 140 150
SVKLFLDYLD LHRGNPSLDI QMIPVSVMFG RAPGREDHKG APQLRLLNGI
160 170 180 190 200
QKFFAVLWLG RDSFVRFSNT VSLRYMADEH GTDKTIAQKL ARVARMHFSR
210 220 230 240 250
LRLAAVGPRL PDRQALFNKL LGSKAIEKAV EDEARSKKIS REKAQQNAVA
260 270 280 290 300
LMEEIAADFT YEAVRLSDRV LSWTWNRLYQ GINVHNAERV RQLAQDGHEI
310 320 330 340 350
VYVPCHRSHM DYLLLSYVLY HQGLVPPHIA AGINLNFWPA GPIFRRLGAF
360 370 380 390 400
FIRRTFKGNK LYSTVFREYL GELFSRGYSV EYFMEGGRSR TGRLLEPKTG
410 420 430 440 450
TLAMTLQAML RGGKRPITLV PVYIGYEHVM EVATYAKELR GATKEKESLP
460 470 480 490 500
QMVRGLRKLR NLGQGYVNFG EPISLNVWLN QHVPEWREAI DPIEAQRPHW
510 520 530 540 550
LPASVNSIAG EVMVNINKAA AANAMNLCAT ALLASRQRAL TREQLLEQLE
560 570 580 590 600
CYLQLLQNVP YAPDATLPQR TPQELLDHAL QMNKFEVEKD NIGDLIILPR
610 620 630 640 650
EQAVLMTYYR NNIQHMLVLP ALVASMVIHH RQISRDELLR QAAVIYPMLK
660 670 680 690 700
QELFMHYVPE TLPQVLSPII DELCRQQLIS LQDDTLIINP PRIRSLQLLA
710 720 730 740 750
AGVRETLQRY AITFSLLSAN PSISRGALEK ESRILAQRLS LLHGINAPEF
760 770 780 790 800
FDKAVFATLV ATLRAEGYIN DVGDAVREQT LEIYNLLADL LTPEIRLTIE
810
SVSIAALEDT GGADGGTA
Length:818
Mass (Da):92,827
Last modified:July 28, 2009 - v1
Checksum:iC22D7ADC3D55C290
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001600 Genomic DNA. Translation: ACR67468.1.
RefSeqiWP_015869677.1. NC_012779.2.

Genome annotation databases

EnsemblBacteriaiACR67468; ACR67468; NT01EI_0225.
GeneIDi7960249.
KEGGieic:NT01EI_0225.
PATRICi21833728. VBIEdwIct114273_0201.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001600 Genomic DNA. Translation: ACR67468.1.
RefSeqiWP_015869677.1. NC_012779.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi634503.NT01EI_0225.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACR67468; ACR67468; NT01EI_0225.
GeneIDi7960249.
KEGGieic:NT01EI_0225.
PATRICi21833728. VBIEdwIct114273_0201.

Phylogenomic databases

eggNOGiENOG4105E55. Bacteria.
COG2937. LUCA.
HOGENOMiHOG000218231.
KOiK00631.
OMAiEVIYVPC.
OrthoDBiEOG6B625B.

Enzyme and pathway databases

UniPathwayiUPA00557; UER00612.
BioCyciEICT634503:GCMY-224-MONOMER.

Family and domain databases

HAMAPiMF_00393. Glyc3P_acyltrans.
InterProiIPR022284. GPAT/DHAPAT.
IPR028354. GPAT_PlsB.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PANTHERiPTHR12563. PTHR12563. 2 hits.
PfamiPF01553. Acyltransferase. 1 hit.
[Graphical view]
PIRSFiPIRSF500064. GPAT. 1 hit.
PIRSF000437. GPAT_DHAPAT. 1 hit.
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03703. plsB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of Edwardsiella ictaluri 93-146."
    Williams M.L., Gillaspy A.F., Dyer D.W., Thune R.L., Waldbieser G.C., Schuster S.C., Gipson J., Zaitshik J., Landry C., Lawrence M.L.
    Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 93-146.

Entry informationi

Entry nameiPLSB_EDWI9
AccessioniPrimary (citable) accession number: C5B706
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: November 11, 2015
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.