C5B706 (PLSB_EDWI9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-3-phosphate acyltransferase
Short name=GPAT
EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	NT01EI_0225

Organism

Edwardsiella ictaluri (strain 93-146) [Complete proteome] [HAMAP]

Taxonomic identifier

634503 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Edwardsiella

Protein attributes

Sequence length	818 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP MF_00393.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity. HAMAP MF_00393
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 818	818	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_1000205849
Regions
	Motif	305 – 310	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	C5B706 [UniParc]. Last modified July 28, 2009. Version 1. Checksum: C22D7ADC3D55C290	FASTA	818	92,827
	10 20 30 40 50 60 MSGWRKIYYK LLNLILKLLV KSKVIPTDPV AELRLDTTRP VFYVLPYNSK VDLLTLRDRC 70 80 90 100 110 120 LALDLPDPLD DNEIDGVILP RYVFIDDGPR VFRYYAPKQA SVKLFLDYLD LHRGNPSLDI 130 140 150 160 170 180 QMIPVSVMFG RAPGREDHKG APQLRLLNGI QKFFAVLWLG RDSFVRFSNT VSLRYMADEH 190 200 210 220 230 240 GTDKTIAQKL ARVARMHFSR LRLAAVGPRL PDRQALFNKL LGSKAIEKAV EDEARSKKIS 250 260 270 280 290 300 REKAQQNAVA LMEEIAADFT YEAVRLSDRV LSWTWNRLYQ GINVHNAERV RQLAQDGHEI 310 320 330 340 350 360 VYVPCHRSHM DYLLLSYVLY HQGLVPPHIA AGINLNFWPA GPIFRRLGAF FIRRTFKGNK 370 380 390 400 410 420 LYSTVFREYL GELFSRGYSV EYFMEGGRSR TGRLLEPKTG TLAMTLQAML RGGKRPITLV 430 440 450 460 470 480 PVYIGYEHVM EVATYAKELR GATKEKESLP QMVRGLRKLR NLGQGYVNFG EPISLNVWLN 490 500 510 520 530 540 QHVPEWREAI DPIEAQRPHW LPASVNSIAG EVMVNINKAA AANAMNLCAT ALLASRQRAL 550 560 570 580 590 600 TREQLLEQLE CYLQLLQNVP YAPDATLPQR TPQELLDHAL QMNKFEVEKD NIGDLIILPR 610 620 630 640 650 660 EQAVLMTYYR NNIQHMLVLP ALVASMVIHH RQISRDELLR QAAVIYPMLK QELFMHYVPE 670 680 690 700 710 720 TLPQVLSPII DELCRQQLIS LQDDTLIINP PRIRSLQLLA AGVRETLQRY AITFSLLSAN 730 740 750 760 770 780 PSISRGALEK ESRILAQRLS LLHGINAPEF FDKAVFATLV ATLRAEGYIN DVGDAVREQT 790 800 810 LEIYNLLADL LTPEIRLTIE SVSIAALEDT GGADGGTA

References

[1]

"Complete genome sequence of Edwardsiella ictaluri 93-146." Williams M.L., Gillaspy A.F., Dyer D.W., Thune R.L., Waldbieser G.C., Schuster S.C., Gipson J., Zaitshik J., Landry C., Lawrence M.L. Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 93-146.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001600 Genomic DNA. Translation: ACR67468.1.
RefSeq	YP_002931703.1. NC_012779.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	C5B706.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7960249.
GenomeReviews	Gene locus NT01EI_0225 in contig CP001600_GR.
KEGG	eic:NT01EI_0225.
PATRIC	21833728. VBIEdwIct114273_0201.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	WNKLYQG.
ProtClustDB	PRK04974.
Family and domain databases
HAMAP	MF_00393. Glyc3P_acyltrans. [Tree]
InterPro	IPR002123. Acyltransferase. IPR022284. G3P_O-AcylTrfase. [Graphical view]
KO	K00631.
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
PIRSF	PIRSF000437. GPAT_DHAPAT. 1 hit.
SMART	SM00563. PlsC. 1 hit. [Graphical view]
TIGRFAMs	TIGR03703. PlsB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLSB_EDWI9

Accession

Primary (citable) accession number: C5B706

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	July 28, 2009
Last modified:	January 25, 2012
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program