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Protein

Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutB

Gene

rutB

Organism
Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby releasing one of the pyrimidine rings nitrogen atoms as ammonia and one of its carbons as CO2.UniRule annotation

Catalytic activityi

(Z)-3-ureidoacrylate peracid + H2O = (Z)-3-peroxyaminoacrylate + CO2 + NH3.UniRule annotation
(Z)-3-ureidoacrylate + H2O = (Z)-3-aminoacrylate + CO2 + NH3.UniRule annotation
(Z)-2-methyl-ureidoacrylate peracid + H2O = (Z)-2-methyl-peroxyaminoacrylate + CO2 + NH3.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei183 – 1831NucleophileUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciMEXT272630:GBY6-1591-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutBUniRule annotation (EC:3.5.1.110UniRule annotation)
Alternative name(s):
Ureidoacrylate amidohydrolaseUniRule annotation
Gene namesi
Name:rutBUniRule annotation
Ordered Locus Names:MexAM1_META1p1663
OrganismiMethylobacterium extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1)
Taxonomic identifieri272630 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
Proteomesi
  • UP000009081 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 248248Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutBPRO_0000402692Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272630.MexAM1_META1p1663.

Structurei

3D structure databases

ProteinModelPortaliC5B0U8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the isochorismatase family. RutB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4106XMA. Bacteria.
COG1335. LUCA.
HOGENOMiHOG000078669.
KOiK09020.
OMAiLAKGGWD.

Family and domain databases

Gene3Di3.40.50.850. 1 hit.
HAMAPiMF_00830. RutB. 1 hit.
InterProiIPR019916. IsoChorase_RutB.
IPR000868. Isochorismatase-like.
[Graphical view]
PfamiPF00857. Isochorismatase. 1 hit.
[Graphical view]
SUPFAMiSSF52499. SSF52499. 1 hit.
TIGRFAMsiTIGR03614. RutB. 1 hit.

Sequencei

Sequence statusi: Complete.

C5B0U8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDADAPAGYR DPSGRHGAVT LPARPEPVAF DADATVLIVV DMQNAYATKG
60 70 80 90 100
GYLDLAGFDV SATGPVIERI ARAVAAARAA GIRVVWFQNG WDPDYVEAGG
110 120 130 140 150
PGSPNWHKSN ALKTMRLRPQ MNQRLLAKGT WDYALVDALT PEPGDIVLPK
160 170 180 190 200
PRYSGFYNTP LDSMLRARGI RTLVFTGIAT NVCVESTLRD GYHREYFGIV
210 220 230 240
LADATHQAGP PALQEGALRN IETFFGWVSD VAAFEAALSS DEARRIPA
Length:248
Mass (Da):26,787
Last modified:July 28, 2009 - v1
Checksum:iB86E9FB723FEFBFF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001510 Genomic DNA. Translation: ACS39512.1.
RefSeqiWP_012752595.1. NC_012808.1.

Genome annotation databases

EnsemblBacteriaiACS39512; ACS39512; MexAM1_META1p1663.
KEGGimea:Mex_1p1663.
PATRICi22509154. VBIMetExt101010_1652.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001510 Genomic DNA. Translation: ACS39512.1.
RefSeqiWP_012752595.1. NC_012808.1.

3D structure databases

ProteinModelPortaliC5B0U8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272630.MexAM1_META1p1663.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACS39512; ACS39512; MexAM1_META1p1663.
KEGGimea:Mex_1p1663.
PATRICi22509154. VBIMetExt101010_1652.

Phylogenomic databases

eggNOGiENOG4106XMA. Bacteria.
COG1335. LUCA.
HOGENOMiHOG000078669.
KOiK09020.
OMAiLAKGGWD.

Enzyme and pathway databases

BioCyciMEXT272630:GBY6-1591-MONOMER.

Family and domain databases

Gene3Di3.40.50.850. 1 hit.
HAMAPiMF_00830. RutB. 1 hit.
InterProiIPR019916. IsoChorase_RutB.
IPR000868. Isochorismatase-like.
[Graphical view]
PfamiPF00857. Isochorismatase. 1 hit.
[Graphical view]
SUPFAMiSSF52499. SSF52499. 1 hit.
TIGRFAMsiTIGR03614. RutB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRUTB_METEA
AccessioniPrimary (citable) accession number: C5B0U8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: July 28, 2009
Last modified: September 7, 2016
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.