C5AZJ8 (C5AZJ8_METEA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Leucine--tRNA ligase HAMAP-Rule MF_00049
EC=6.1.1.4 HAMAP-Rule MF_00049

Alternative name(s):
Leucyl-tRNA synthetase HAMAP-Rule MF_00049

Gene names

Name:	leuS HAMAP-Rule MF_00049 EMBL ACS39332.1
Ordered Locus Names:	MexAM1_META1p1470 EMBL ACS39332.1

Organism

Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1) [Complete proteome] [HAMAP] EMBL ACS39332.1

Taxonomic identifier

272630 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium › Methylobacterium extorquens group › Methylobacterium extorquens

Protein attributes

Sequence length	875 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00049.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. HAMAP-Rule MF_00049 RuleBase RU003489

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP-Rule MF_00049 RuleBase RU003489
Cellular component	Cytoplasm HAMAP-Rule MF_00049
Ligand	ATP-binding HAMAP-Rule MF_00049 RuleBase RU003489 Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase HAMAP-Rule MF_00049 RuleBase RU003489 EMBL ACS39332.1 Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	leucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro leucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Motif	56 – 66	11	"HIGH" region By similarity HAMAP-Rule MF_00049
	Motif	644 – 648	5	"KMSKS" region By similarity HAMAP-Rule MF_00049
Sites
	Binding site	647	1	ATP By similarity HAMAP-Rule MF_00049

Sequences

	Sequence		Length	Mass (Da)
	C5AZJ8 [UniParc]. Last modified July 28, 2009. Version 1. Checksum: BF494B79EA51E6F5	FASTA	875	97,337
	10 20 30 40 50 60 MTDSAQPSPT AQERHQERYN AKDAEPRWQQ AWDAAKLFET KNDDPRPKYY VLEMFPYPSG 70 80 90 100 110 120 RIHIGHVRNY AMGDVVARYK RAKGHNVLHP MGWDAFGLPA ENAAMERKVN PREWTYANIA 130 140 150 160 170 180 TMRGQLQSMG LSLDWSREIA TCDPDYYKHQ QRMFLDFLGK GLVTRRTAKV NWDPVDHTVL 190 200 210 220 230 240 ANEQVIDGRG WRSGALVEQR ELTQWFFKIT DFAQDLHDAL DGLERWPEKV RLMQRNWIGR 250 260 270 280 290 300 SEGLEVRFAL AAPFAGTDEV TVYTTRPDTL FGAKFLAISV DHPLARALAE SGPKAAELQA 310 320 330 340 350 360 FAEECRRIGT AQEAIDTAEK LGFDTGLTVR HPLDPSWELP VFVANFVLME YGTGAVFGCP 370 380 390 400 410 420 AHDQRDLDFA NKYGLGNTPV VCPEGQDPDA FIITDTAYDG DGRMIHSRFL DGMTTDEAFK 430 440 450 460 470 480 AVADRLEGET LGDAPVARRK VQFRLRDWGV SRQRYWGCPI PIIHCEACGP VPVPVADLPV 490 500 510 520 530 540 KLPDEVSFDQ PGNPLEREQA WRQVPCPRCG AAARRETDTM DTFVDSSWYY ARFTAPWLAD 550 560 570 580 590 600 APTDRKTVDH WLAVDQYIGG VEHAILHLLY SRFFMRAMQA TGWSGVSEPF AGLFTQGMVV 610 620 630 640 650 660 HETYKDAAGA WTPPAEIRFE TQDGARKAFH VKTGEPIEIG PTEKMSKSKK NVVDPDDIIA 670 680 690 700 710 720 SYGADTARWF MLSDSPPERD VIWTEEGVQG AARFVQRVWR LVHGATAANG PGGTADAPLR 730 740 750 760 770 780 KAAHKALAAV GEDIERLRFN RCVAHIYTLA NALDEGLRGG ASKAAAGEAA RILVQLIAPM 790 800 810 820 830 840 MPHLAEESWR VLGGEGLVAE TAWPVADQAL LVEDEITLPV QINGKKRADV TVPRDADAKA 850 860 870 VEAATLALEP VQRALEGRAP RKVIVVPGRI VNLVV

References

[1]

"Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources." Vuilleumier S., Chistoserdova L., Lee M.C., Bringel F., Lajus A., Zhou Y., Gourion B., Barbe V., Chang J., Cruveiller S., Dossat C., Gillett W., Gruffaz C., Haugen E., Hourcade E., Levy R., Mangenot S., Muller E. , Nadalig T., Pagni M., Penny C., Peyraud R., Robinson D.G., Roche D., Rouy Z., Saenampechek C., Salvignol G., Vallenet D., Wu Z., Marx C.J., Vorholt J.A., Olson M.V., Kaul R., Weissenbach J., Medigue C., Lidstrom M.E. PLoS ONE 4:E5584-E5584(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 14718 / DSM 1338 / AM1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001510 Genomic DNA. Translation: ACS39332.1.
RefSeq	YP_002962609.1. NC_012808.1.
3D structure databases
ProteinModelPortal	C5AZJ8.
ModBase	Search...
Protein-protein interaction databases
STRING	272630.MexAM1_META1p1470.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACS39332; ACS39332; MexAM1_META1p1470.
GeneID	7991449.
KEGG	mea:Mex_1p1470.
PATRIC	22508779. VBIMetExt101010_1466.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0495.
HOGENOM	HOG000200747.
KO	K01869.
OMA	GWPEQVK.
ProtClustDB	PRK00390.
Family and domain databases
Gene3D	3.40.50.620. 3 hits. 3.90.740.10. 1 hit.
HAMAP	MF_00049_B. Leu_tRNA_synth_B.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002302. Leu-tRNA-ligase_bac/mito. IPR025709. Leu_tRNA-synth_edit. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
PANTHER	PTHR11946:SF7. PTHR11946:SF7. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 3 hits. PF13603. tRNA-synt_1_2. 1 hit. [Graphical view]
PRINTS	PR00985. TRNASYNTHLEU.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00396. leuS_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C5AZJ8_METEA

Accession

Primary (citable) accession number: C5AZJ8

Entry history

Integrated into UniProtKB/TrEMBL:	July 28, 2009
Last sequence update:	July 28, 2009
Last modified:	April 3, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)