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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei647ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetaseUniRule annotationImported, Ligase

Keywords - Biological processi

Protein biosynthesisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Gene namesi
Name:leuSUniRule annotationImported
Ordered Locus Names:MexAM1_META1p1470Imported
OrganismiMethylobacterium extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1)Imported
Taxonomic identifieri272630 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
Proteomesi
  • UP000009081 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi272630.MexAM1_META1p1470.

Structurei

3D structure databases

ProteinModelPortaliC5AZJ8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 185tRNA-synt_1InterPro annotationAdd BLAST158
Domaini235 – 427tRNA-synt_1_2InterPro annotationAdd BLAST193
Domaini446 – 602tRNA-synt_1InterPro annotationAdd BLAST157
Domaini643 – 682tRNA-synt_1InterPro annotationAdd BLAST40
Domaini721 – 841Anticodon_1InterPro annotationAdd BLAST121

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi56 – 66"HIGH" regionUniRule annotationAdd BLAST11
Motifi644 – 648"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C8T. Bacteria.
COG0495. LUCA.
HOGENOMiHOG000200747.
KOiK01869.
OMAiHRFIKRL.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 4 hits.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C5AZJ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDSAQPSPT AQERHQERYN AKDAEPRWQQ AWDAAKLFET KNDDPRPKYY
60 70 80 90 100
VLEMFPYPSG RIHIGHVRNY AMGDVVARYK RAKGHNVLHP MGWDAFGLPA
110 120 130 140 150
ENAAMERKVN PREWTYANIA TMRGQLQSMG LSLDWSREIA TCDPDYYKHQ
160 170 180 190 200
QRMFLDFLGK GLVTRRTAKV NWDPVDHTVL ANEQVIDGRG WRSGALVEQR
210 220 230 240 250
ELTQWFFKIT DFAQDLHDAL DGLERWPEKV RLMQRNWIGR SEGLEVRFAL
260 270 280 290 300
AAPFAGTDEV TVYTTRPDTL FGAKFLAISV DHPLARALAE SGPKAAELQA
310 320 330 340 350
FAEECRRIGT AQEAIDTAEK LGFDTGLTVR HPLDPSWELP VFVANFVLME
360 370 380 390 400
YGTGAVFGCP AHDQRDLDFA NKYGLGNTPV VCPEGQDPDA FIITDTAYDG
410 420 430 440 450
DGRMIHSRFL DGMTTDEAFK AVADRLEGET LGDAPVARRK VQFRLRDWGV
460 470 480 490 500
SRQRYWGCPI PIIHCEACGP VPVPVADLPV KLPDEVSFDQ PGNPLEREQA
510 520 530 540 550
WRQVPCPRCG AAARRETDTM DTFVDSSWYY ARFTAPWLAD APTDRKTVDH
560 570 580 590 600
WLAVDQYIGG VEHAILHLLY SRFFMRAMQA TGWSGVSEPF AGLFTQGMVV
610 620 630 640 650
HETYKDAAGA WTPPAEIRFE TQDGARKAFH VKTGEPIEIG PTEKMSKSKK
660 670 680 690 700
NVVDPDDIIA SYGADTARWF MLSDSPPERD VIWTEEGVQG AARFVQRVWR
710 720 730 740 750
LVHGATAANG PGGTADAPLR KAAHKALAAV GEDIERLRFN RCVAHIYTLA
760 770 780 790 800
NALDEGLRGG ASKAAAGEAA RILVQLIAPM MPHLAEESWR VLGGEGLVAE
810 820 830 840 850
TAWPVADQAL LVEDEITLPV QINGKKRADV TVPRDADAKA VEAATLALEP
860 870
VQRALEGRAP RKVIVVPGRI VNLVV
Length:875
Mass (Da):97,337
Last modified:July 28, 2009 - v1
Checksum:iBF494B79EA51E6F5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001510 Genomic DNA. Translation: ACS39332.1.
RefSeqiWP_012752502.1. NC_012808.1.

Genome annotation databases

EnsemblBacteriaiACS39332; ACS39332; MexAM1_META1p1470.
KEGGimea:Mex_1p1470.
PATRICi22508779. VBIMetExt101010_1466.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001510 Genomic DNA. Translation: ACS39332.1.
RefSeqiWP_012752502.1. NC_012808.1.

3D structure databases

ProteinModelPortaliC5AZJ8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272630.MexAM1_META1p1470.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACS39332; ACS39332; MexAM1_META1p1470.
KEGGimea:Mex_1p1470.
PATRICi22508779. VBIMetExt101010_1466.

Phylogenomic databases

eggNOGiENOG4105C8T. Bacteria.
COG0495. LUCA.
HOGENOMiHOG000200747.
KOiK01869.
OMAiHRFIKRL.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 4 hits.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiC5AZJ8_METEA
AccessioniPrimary (citable) accession number: C5AZJ8
Entry historyi
Integrated into UniProtKB/TrEMBL: July 28, 2009
Last sequence update: July 28, 2009
Last modified: November 2, 2016
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.