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C5AWP4 (C5AWP4_METEA) Unreviewed, UniProtKB/TrEMBL

Last modified May 29, 2013. Version 32. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047
EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddlB EMBL ACS40899.1
Synonyms:ddl HAMAP-Rule MF_00047
Ordered Locus Names:MexAM1_META1p3151 EMBL ACS40899.1
OrganismMethylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1) [Complete proteome] [HAMAP] EMBL ACS40899.1
Taxonomic identifier272630 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium ›

Protein attributes

Sequence length307 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS000291

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS000291

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS000291

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS000291

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS000291.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047 SAAS SAAS000291

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain101 – 301201ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding127 – 18256ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2511Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2681Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2681Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2701Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
C5AWP4 [UniParc].

Last modified July 28, 2009. Version 1.
Checksum: 851F27163875763C

FASTA30732,802
        10         20         30         40         50         60 
MSKHVAVLMG GWSSEREISL RSGNACAEAL EGEGYRVTRV DVGPDIATVL TELRPDAALN 

        70         80         90        100        110        120 
ALHGPAGEDG TIQGLLEILK IPYTHSGVLA SALAMHKERA KTVMRAAGVS VPEGRVVNRH 

       130        140        150        160        170        180 
DAAKSHPLTP PYVVKPIAEG SSMGVIIVRD ERSHPPQILA SDEWVYGEEV LAETYVAGRE 

       190        200        210        220        230        240 
LTCAVLGDRA LGVTEIKPVS GEWYDFDAKY AGGGSIHVLP ADLKLNIYQR VQELALTAHQ 

       250        260        270        280        290        300 
ALGCRGVSRA DLRYDDTPGG TGALVVLEVN TQPGMTQTSL VPEIAAHAGQ SFGELVRWMV 


EDASLNR

« Hide

References

[1]"Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources."
Vuilleumier S., Chistoserdova L., Lee M.C., Bringel F., Lajus A., Zhou Y., Gourion B., Barbe V., Chang J., Cruveiller S., Dossat C., Gillett W., Gruffaz C., Haugen E., Hourcade E., Levy R., Mangenot S., Muller E. expand/collapse author list , Nadalig T., Pagni M., Penny C., Peyraud R., Robinson D.G., Roche D., Rouy Z., Saenampechek C., Salvignol G., Vallenet D., Wu Z., Marx C.J., Vorholt J.A., Olson M.V., Kaul R., Weissenbach J., Medigue C., Lidstrom M.E.
PLoS ONE 4:E5584-E5584(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 14718 / DSM 1338 / AM1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001510 Genomic DNA. Translation: ACS40899.1.
RefSeqYP_002964176.1. NC_012808.1.

3D structure databases

ProteinModelPortalC5AWP4.
ModBaseSearch...

Protein-protein interaction databases

STRING272630.MexAM1_META1p3151.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACS40899; ACS40899; MexAM1_META1p3151.
GeneID7993034.
KEGGmea:Mex_1p3151.
PATRIC22512037. VBIMetExt101010_3082.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMAKYTEGFA.
ProtClustDBPRK01372.

Enzyme and pathway databases

BioCycMEXT272630:GBY6-2994-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameC5AWP4_METEA
AccessionPrimary (citable) accession number: C5AWP4
Entry history
Integrated into UniProtKB/TrEMBL: July 28, 2009
Last sequence update: July 28, 2009
Last modified: May 29, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info