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Protein

UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase

Gene

murF

Organism
Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein.UniRule annotation

Catalytic activityi

ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity Source: InterPro
  3. UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. cell wall organization Source: UniProtKB-KW
  4. peptidoglycan biosynthetic process Source: UniProtKB-UniPathway
  5. regulation of cell shape Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Biological processi

Cell cycle, Cell divisionUniRule annotation, Cell shape, Cell wall biogenesis/degradationUniRule annotation, Peptidoglycan synthesisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciMEXT272630:GBY6-4812-MONOMER.
UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligaseUniRule annotation (EC:6.3.2.10UniRule annotation)
Gene namesi
Name:murFImported
Ordered Locus Names:MexAM1_META1p5086Imported
OrganismiMethylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1)Imported
Taxonomic identifieri272630 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
ProteomesiUP000009081 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Interactioni

Protein-protein interaction databases

STRINGi272630.MexAM1_META1p5086.

Family & Domainsi

Sequence similaritiesi

Belongs to the MurCDEF family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0770.
HOGENOMiHOG000268120.
KOiK01929.
OMAiGNRNNEI.
OrthoDBiEOG6PKFCR.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.1390.10. 1 hit.
3.90.190.20. 1 hit.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005863. UDP-N-AcMur-pentapeptide_synth.
[Graphical view]
PANTHERiPTHR23135:SF3. PTHR23135:SF3. 1 hit.
PfamiPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
SSF63418. SSF63418. 1 hit.
TIGRFAMsiTIGR01143. murF. 1 hit.

Sequencei

Sequence statusi: Complete.

C5ATZ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDAPLWTPA ALEAATGGRL IGAPRAVTGA SIDTRSLEPG DLFFAIRGEA
60 70 80 90 100
RDGHDFVRAA LEKGAGAAVV AEARAKEFEG AGPVLAVPGE GDDPVLDAMR
110 120 130 140 150
RLGLAARART GAAIVAVTGS VGKTGTKEAL RHVLSAQGAT HASVASYNNH
160 170 180 190 200
WGVPLTLTRM LEDTRFGVFE IGMNHGAEIL PLTAMVRPDV ALITTVEPVH
210 220 230 240 250
IEHFRSLSAI ADAKGEIFSG LKPGGVAVIN RDNPNYERLL AHAQASRAGR
260 270 280 290 300
VITFGEHAAA DVCAHRILTR PDVSVVDATV MGVPVTYQLG TPGRHVAMNS
310 320 330 340 350
LGVLACIHAL GADLARAALS LASLKPPVGR GERTALRIGD GEAFLVDESY
360 370 380 390 400
NANPASIRAA LSTLAGIDTG AKGRRIAVLG DMKELGAAGE ALHRELADAV
410 420 430 440 450
AANGIDLVFA VGPLMAHLFE ALPMPVRGTA APTAEGVTDA VLATLRSGDA
460 470 480
VMVKGSNSMR MVRIVEAVKA RYAVAPDPRE ASLNAVR
Length:487
Mass (Da):50,405
Last modified:July 28, 2009 - v1
Checksum:i946DA594CB00F681
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001510 Genomic DNA. Translation: ACS42690.1.
RefSeqiWP_003603656.1. NC_012808.1.
YP_002965967.1. NC_012808.1.

Genome annotation databases

EnsemblBacteriaiACS42690; ACS42690; MexAM1_META1p5086.
GeneIDi7994853.
KEGGimea:Mex_1p5086.
PATRICi22515823. VBIMetExt101010_4960.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001510 Genomic DNA. Translation: ACS42690.1.
RefSeqiWP_003603656.1. NC_012808.1.
YP_002965967.1. NC_012808.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272630.MexAM1_META1p5086.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACS42690; ACS42690; MexAM1_META1p5086.
GeneIDi7994853.
KEGGimea:Mex_1p5086.
PATRICi22515823. VBIMetExt101010_4960.

Phylogenomic databases

eggNOGiCOG0770.
HOGENOMiHOG000268120.
KOiK01929.
OMAiGNRNNEI.
OrthoDBiEOG6PKFCR.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciMEXT272630:GBY6-4812-MONOMER.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.1390.10. 1 hit.
3.90.190.20. 1 hit.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005863. UDP-N-AcMur-pentapeptide_synth.
[Graphical view]
PANTHERiPTHR23135:SF3. PTHR23135:SF3. 1 hit.
PfamiPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
SSF63418. SSF63418. 1 hit.
TIGRFAMsiTIGR01143. murF. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 14718 / DSM 1338 / AM1Imported.

Entry informationi

Entry nameiC5ATZ1_METEA
AccessioniPrimary (citable) accession number: C5ATZ1
Entry historyi
Integrated into UniProtKB/TrEMBL: July 28, 2009
Last sequence update: July 28, 2009
Last modified: April 1, 2015
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.