C5ATZ1 (C5ATZ1_METEA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase RuleBase RU004136
EC=6.3.2.10 RuleBase RU004136

Gene names

Name:	murF EMBL ACS42690.1
Ordered Locus Names:	MexAM1_META1p5086 EMBL ACS42690.1

Organism

Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1) [Complete proteome] [HAMAP] EMBL ACS42690.1

Taxonomic identifier

272630 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium › Methylobacterium extorquens group › Methylobacterium extorquens

Protein attributes

Sequence length	487 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the MurCDEF family. RuleBase RU003630

Ontologies

Keywords
Biological process	Cell cycle Cell division RuleBase RU004136 Cell shape Cell wall biogenesis/degradation RuleBase RU004136 Peptidoglycan synthesis RuleBase RU004136
Ligand	ATP-binding RuleBase RU003630 Nucleotide-binding
Molecular function	Ligase RuleBase RU003630 EMBL ACS42690.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity Inferred from electronic annotation. Source: EC UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	C5ATZ1 [UniParc]. Last modified July 28, 2009. Version 1. Checksum: 946DA594CB00F681	FASTA	487	50,405
	10 20 30 40 50 60 MSDAPLWTPA ALEAATGGRL IGAPRAVTGA SIDTRSLEPG DLFFAIRGEA RDGHDFVRAA 70 80 90 100 110 120 LEKGAGAAVV AEARAKEFEG AGPVLAVPGE GDDPVLDAMR RLGLAARART GAAIVAVTGS 130 140 150 160 170 180 VGKTGTKEAL RHVLSAQGAT HASVASYNNH WGVPLTLTRM LEDTRFGVFE IGMNHGAEIL 190 200 210 220 230 240 PLTAMVRPDV ALITTVEPVH IEHFRSLSAI ADAKGEIFSG LKPGGVAVIN RDNPNYERLL 250 260 270 280 290 300 AHAQASRAGR VITFGEHAAA DVCAHRILTR PDVSVVDATV MGVPVTYQLG TPGRHVAMNS 310 320 330 340 350 360 LGVLACIHAL GADLARAALS LASLKPPVGR GERTALRIGD GEAFLVDESY NANPASIRAA 370 380 390 400 410 420 LSTLAGIDTG AKGRRIAVLG DMKELGAAGE ALHRELADAV AANGIDLVFA VGPLMAHLFE 430 440 450 460 470 480 ALPMPVRGTA APTAEGVTDA VLATLRSGDA VMVKGSNSMR MVRIVEAVKA RYAVAPDPRE ASLNAVR

References

[1]

"Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources." Vuilleumier S., Chistoserdova L., Lee M.C., Bringel F., Lajus A., Zhou Y., Gourion B., Barbe V., Chang J., Cruveiller S., Dossat C., Gillett W., Gruffaz C., Haugen E., Hourcade E., Levy R., Mangenot S., Muller E. , Nadalig T., Pagni M., Penny C., Peyraud R., Robinson D.G., Roche D., Rouy Z., Saenampechek C., Salvignol G., Vallenet D., Wu Z., Marx C.J., Vorholt J.A., Olson M.V., Kaul R., Weissenbach J., Medigue C., Lidstrom M.E. PLoS ONE 4:E5584-E5584(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 14718 / DSM 1338 / AM1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001510 Genomic DNA. Translation: ACS42690.1.
RefSeq	YP_002965967.1. NC_012808.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	272630.MexAM1_META1p5086.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACS42690; ACS42690; MexAM1_META1p5086.
GeneID	7994853.
KEGG	mea:Mex_1p5086.
PATRIC	22515823. VBIMetExt101010_4960.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0770.
HOGENOM	HOG000268120.
KO	K01929.
OMA	HCVGPRM.
ProtClustDB	CLSK2332278.
Enzyme and pathway databases
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.40.1190.10. 1 hit. 3.90.190.20. 1 hit.
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005863. UDP-N-AcMur-pentapeptide_synth. [Graphical view]
PANTHER	PTHR23135:SF3. PTHR23135:SF3. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01143. murF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

C5ATZ1_METEA

Accession

Primary (citable) accession number: C5ATZ1

Entry history

Integrated into UniProtKB/TrEMBL:	July 28, 2009
Last sequence update:	July 28, 2009
Last modified:	April 3, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)