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Protein

UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase

Gene

murF

Organism
Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein.UniRule annotation

Catalytic activityi

ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine.UniRule annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Biological processi

Cell cycle, Cell divisionUniRule annotation, Cell shape, Cell wall biogenesis/degradationUniRule annotation, Peptidoglycan synthesisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciMEXT272630:GBY6-4812-MONOMER.
UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligaseUniRule annotation (EC:6.3.2.10UniRule annotation)
Alternative name(s):
D-alanyl-D-alanine-adding enzymeUniRule annotation
Gene namesi
Name:murFUniRule annotationImported
Ordered Locus Names:MexAM1_META1p5086Imported
OrganismiMethylobacterium extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1)Imported
Taxonomic identifieri272630 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
Proteomesi
  • UP000009081 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi272630.MexAM1_META1p5086.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 10680Mur_ligaseInterPro annotationAdd
BLAST
Domaini117 – 306190Mur_ligase_MInterPro annotationAdd
BLAST
Domaini344 – 40966Mur_ligase_CInterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the MurCDEF family. MurF subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107EES. Bacteria.
COG0770. LUCA.
HOGENOMiHOG000268120.
KOiK01929.
OMAiDYAVIEM.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.1390.10. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_02019. MurF. 1 hit.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005863. UDP-N-AcMur_synth.
[Graphical view]
PANTHERiPTHR23135:SF3. PTHR23135:SF3. 1 hit.
PfamiPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
SSF63418. SSF63418. 1 hit.
TIGRFAMsiTIGR01143. murF. 1 hit.

Sequencei

Sequence statusi: Complete.

C5ATZ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDAPLWTPA ALEAATGGRL IGAPRAVTGA SIDTRSLEPG DLFFAIRGEA
60 70 80 90 100
RDGHDFVRAA LEKGAGAAVV AEARAKEFEG AGPVLAVPGE GDDPVLDAMR
110 120 130 140 150
RLGLAARART GAAIVAVTGS VGKTGTKEAL RHVLSAQGAT HASVASYNNH
160 170 180 190 200
WGVPLTLTRM LEDTRFGVFE IGMNHGAEIL PLTAMVRPDV ALITTVEPVH
210 220 230 240 250
IEHFRSLSAI ADAKGEIFSG LKPGGVAVIN RDNPNYERLL AHAQASRAGR
260 270 280 290 300
VITFGEHAAA DVCAHRILTR PDVSVVDATV MGVPVTYQLG TPGRHVAMNS
310 320 330 340 350
LGVLACIHAL GADLARAALS LASLKPPVGR GERTALRIGD GEAFLVDESY
360 370 380 390 400
NANPASIRAA LSTLAGIDTG AKGRRIAVLG DMKELGAAGE ALHRELADAV
410 420 430 440 450
AANGIDLVFA VGPLMAHLFE ALPMPVRGTA APTAEGVTDA VLATLRSGDA
460 470 480
VMVKGSNSMR MVRIVEAVKA RYAVAPDPRE ASLNAVR
Length:487
Mass (Da):50,405
Last modified:July 28, 2009 - v1
Checksum:i946DA594CB00F681
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001510 Genomic DNA. Translation: ACS42690.1.
RefSeqiWP_003603656.1. NC_012808.1.

Genome annotation databases

EnsemblBacteriaiACS42690; ACS42690; MexAM1_META1p5086.
KEGGimea:Mex_1p5086.
PATRICi22515823. VBIMetExt101010_4960.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001510 Genomic DNA. Translation: ACS42690.1.
RefSeqiWP_003603656.1. NC_012808.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272630.MexAM1_META1p5086.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACS42690; ACS42690; MexAM1_META1p5086.
KEGGimea:Mex_1p5086.
PATRICi22515823. VBIMetExt101010_4960.

Phylogenomic databases

eggNOGiENOG4107EES. Bacteria.
COG0770. LUCA.
HOGENOMiHOG000268120.
KOiK01929.
OMAiDYAVIEM.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciMEXT272630:GBY6-4812-MONOMER.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.1390.10. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_02019. MurF. 1 hit.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005863. UDP-N-AcMur_synth.
[Graphical view]
PANTHERiPTHR23135:SF3. PTHR23135:SF3. 1 hit.
PfamiPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
SSF63418. SSF63418. 1 hit.
TIGRFAMsiTIGR01143. murF. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiC5ATZ1_METEA
AccessioniPrimary (citable) accession number: C5ATZ1
Entry historyi
Integrated into UniProtKB/TrEMBL: July 28, 2009
Last sequence update: July 28, 2009
Last modified: September 7, 2016
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.