C5AF36 (C5AF36_BURGB) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Methionyl-tRNA formyltransferase HAMAP-Rule MF_00182
EC=2.1.2.9 HAMAP-Rule MF_00182

Gene names

Name:	fmt HAMAP-Rule MF_00182
Ordered Locus Names:	bglu_1g34090 EMBL ACR30471.1

Organism

Burkholderia glumae (strain BGR1) [Complete proteome] [HAMAP] EMBL ACR30471.1

Taxonomic identifier

626418 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia glumae

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by: (I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-GTP By similarity. HAMAP-Rule MF_00182 SAAS SAAS001555
Catalytic activity	10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet). HAMAP-Rule MF_00182 SAAS SAAS001555
Sequence similarities	Belongs to the fmt family. HAMAP-Rule MF_00182

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP-Rule MF_00182 SAAS SAAS001555
Molecular function	Methyltransferase HAMAP-Rule MF_00182 SAAS SAAS001555 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translational initiation Inferred from electronic annotation. Source: GOC
Molecular_function	methionyl-tRNA formyltransferase activity Inferred from electronic annotation. Source: HAMAP methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	121 – 124	4	Tetrahydrofolate (THF) binding By similarity HAMAP-Rule MF_00182

Sequences

	Sequence		Length	Mass (Da)
	C5AF36 [UniParc]. Last modified July 28, 2009. Version 1. Checksum: 8AC4917AA1573395	FASTA	327	34,034
	10 20 30 40 50 60 MTHSLRVIFA GTPEFAAAAL AAIHEAGFAV PLVLTQPDRP AGRGMKLQAS AVKRYALDHG 70 80 90 100 110 120 MPVAQPPSLR RTGKYPAEAA EALDRLRATP HDVMVVAAYG LLLPQEVLEM PPRGCINIHA 130 140 150 160 170 180 SLLPRWRGAA PIHRALEAGD AQTGVTLMQM DVGLDTGAML SEGRVAIAAD ETTASLHDKL 190 200 210 220 230 240 AQTGARLIVE ALVQLERDGE LAATPQPADG VTYAEKIGKH EAALDWREPA AVLARRIRAF 250 260 270 280 290 300 DPFPGGAGTL DGTVIKLWAA EPVVASGAAE PGTIVEAGAG GVVIACGEGA LRVTQLQKPG 310 320 GKRLPAREFL AGAPLAAGQR FALPEAG

References

[1]	"Complete genome sequence of Burkholderia glumae BGR1." Lim J., Lee T.H., Nahm B.H., Choi Y.D., Kim M., Hwang I. J. Bacteriol. 191:3758-3759(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BGR1 EMBL ACR30471.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001503 Genomic DNA. Translation: ACR30471.1.
RefSeq	YP_002913175.1. NC_012724.2.
3D structure databases
ProteinModelPortal	C5AF36.
ModBase	Search...
Protein-protein interaction databases
STRING	626418.bglu_1g34090.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACR30471; ACR30471; bglu_1g34090.
GeneID	7907338.
KEGG	bgl:bglu_1g34090.
PATRIC	19114644. VBIBurGlu130723_6371.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0223.
HOGENOM	HOG000261177.
KO	K00604.
OMA	GITLMQM.
ProtClustDB	PRK00005.
Enzyme and pathway databases
BioCyc	BGLU626418:GJI5-3461-MONOMER.
Family and domain databases
Gene3D	3.10.25.10. 1 hit. 3.40.50.170. 1 hit.
HAMAP	MF_00182. Formyl_trans.
InterPro	IPR005794. Fmt. IPR005793. Formyl_trans_C. IPR002376. Formyl_transf_N. IPR011034. Formyl_transferase_C-like. IPR001555. GART_AS. IPR015518. Met_tRNA_Form_TA-like. [Graphical view]
PANTHER	PTHR11138. PTHR11138. 1 hit.
Pfam	PF02911. Formyl_trans_C. 1 hit. PF00551. Formyl_trans_N. 1 hit. [Graphical view]
SUPFAM	SSF50486. FMT_C_like. 1 hit. SSF53328. formyl_transf. 1 hit.
TIGRFAMs	TIGR00460. fmt. 1 hit.
PROSITE	PS00373. GART. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C5AF36_BURGB

Accession

Primary (citable) accession number: C5AF36

Entry history

Integrated into UniProtKB/TrEMBL:	July 28, 2009
Last sequence update:	July 28, 2009
Last modified:	May 1, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)