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C5AA24

- C5AA24_BURGB

UniProt

C5AA24 - C5AA24_BURGB

Protein

Acetyl-coenzyme A synthetase

Gene

acsA

Organism
Burkholderia glumae (strain BGR1)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 37 (01 Oct 2014)
      Sequence version 1 (28 Jul 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.UniRule annotation

    Catalytic activityi

    ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei317 – 3171Coenzyme AUniRule annotation
    Binding sitei397 – 3971Substrate; via nitrogen amideUniRule annotation
    Binding sitei512 – 5121SubstrateUniRule annotation
    Binding sitei528 – 5281SubstrateUniRule annotation
    Active sitei530 – 5301UniRule annotation
    Binding sitei536 – 5361Coenzyme AUniRule annotation
    Binding sitei539 – 5391SubstrateUniRule annotation
    Metal bindingi550 – 5501Magnesium; via carbonyl oxygenUniRule annotation
    Metal bindingi552 – 5521Magnesium; via carbonyl oxygenUniRule annotation
    Metal bindingi555 – 5551Magnesium; via carbonyl oxygenUniRule annotation
    Binding sitei600 – 6001Coenzyme AUniRule annotation

    GO - Molecular functioni

    1. acetate-CoA ligase activity Source: UniProtKB-HAMAP
    2. AMP binding Source: InterPro
    3. ATP binding Source: UniProtKB-KW
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. acetyl-CoA biosynthetic process from acetate Source: InterPro

    Keywords - Molecular functioni

    LigaseUniRule annotationImported

    Keywords - Ligandi

    ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBGLU626418:GJI5-2579-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acetyl-coenzyme A synthetaseUniRule annotation (EC:6.2.1.1UniRule annotation)
    Short name:
    AcCoA synthetaseUniRule annotation
    Short name:
    AcsUniRule annotation
    Alternative name(s):
    Acetate--CoA ligaseUniRule annotation
    Acyl-activating enzymeUniRule annotation
    Gene namesi
    Name:acsAUniRule annotation
    Ordered Locus Names:bglu_1g25360Imported
    OrganismiBurkholderia glumae (strain BGR1)Imported
    Taxonomic identifieri626418 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderia
    ProteomesiUP000002187: Chromosome 1

    PTM / Processingi

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei625 – 6251N6-acetyllysineUniRule annotation

    Post-translational modificationi

    Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.UniRule annotation

    Keywords - PTMi

    AcetylationUniRule annotation

    Interactioni

    Protein-protein interaction databases

    STRINGi626418.bglu_1g25360.

    Structurei

    3D structure databases

    ProteinModelPortaliC5AA24.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0365.
    HOGENOMiHOG000229981.
    KOiK01895.
    OMAiAWIWYRD.
    OrthoDBiEOG68WR2H.

    Family and domain databases

    HAMAPiMF_01123. Ac_CoA_synth.
    InterProiIPR011904. Ac_CoA_lig.
    IPR025110. AMP-bd_C.
    IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view]
    PfamiPF00501. AMP-binding. 1 hit.
    PF13193. AMP-binding_C. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
    PROSITEiPS00455. AMP_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    C5AA24-1 [UniParc]FASTAAdd to Basket

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    MSAIESVLHE RRQFAPPASV EQAANISGMD AYRALVDEAG RDYEGFWARL    50
    ARESLDWHKP FSSVLDESNA PFYKWFEDGE LNASYNCLDR HVAAGRGERV 100
    AVIFEADDGT VTRVTYAELL ARVCRLANAL KARGIGRGDR VIIYIPMSIE 150
    GIVAMQACAR IGATHSVVFG GFSAKSLNER LVDVGACALI TADEQVRGGK 200
    TLPIKSIADD AIAMGGCEAV KSVIVYRRTG GEVAWHEGRD LWLDEITANQ 250
    PDTCEPEWVG AEHPLFILYT SGSTGKPKGL QHSTGGYLLW AALTMDWTFD 300
    RKPDDVFWCT ADIGWITGHT YITYGPLATC STQVVFEGVP TYPNAGRFWK 350
    MVADHKVTVF YTAPTAIRSL IKASGADEAA HPSRYDLSSL RIIGTVGEPI 400
    NPESWMWYHK NVGGERCPIV DTWFQTETGG HMITPLPGAT PTVPGSCTLG 450
    LPGIMAAVVD ETGQDVPNGQ GGILVIKRPW PAMARTIWGD PDRFRKSYFP 500
    DELGGRLYLA GDGTVRDKDT GYFTIMGRID DVLNVSGHRL GTMEIESALV 550
    SHELVAEAAV VGRPDDMTGE AVVAFVVLKR TRPEGEEAQQ IAKMLRDWVG 600
    KEIGPIAKPK DIRFGDNLPK TRSGKIMRRL LRSLAKGEAV TQDTSTLENP 650
    AILEQLAEAR 660
    Length:660
    Mass (Da):72,189
    Last modified:July 28, 2009 - v1
    Checksum:i2C43F2FA5C9CF54C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001503 Genomic DNA. Translation: ACR29612.1.
    RefSeqiWP_015876482.1. NC_012724.2.
    YP_002912316.1. NC_012724.2.

    Genome annotation databases

    EnsemblBacteriaiACR29612; ACR29612; bglu_1g25360.
    GeneIDi7906710.
    KEGGibgl:bglu_1g25360.
    PATRICi19112782. VBIBurGlu130723_5450.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001503 Genomic DNA. Translation: ACR29612.1 .
    RefSeqi WP_015876482.1. NC_012724.2.
    YP_002912316.1. NC_012724.2.

    3D structure databases

    ProteinModelPortali C5AA24.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 626418.bglu_1g25360.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACR29612 ; ACR29612 ; bglu_1g25360 .
    GeneIDi 7906710.
    KEGGi bgl:bglu_1g25360.
    PATRICi 19112782. VBIBurGlu130723_5450.

    Phylogenomic databases

    eggNOGi COG0365.
    HOGENOMi HOG000229981.
    KOi K01895.
    OMAi AWIWYRD.
    OrthoDBi EOG68WR2H.

    Enzyme and pathway databases

    BioCyci BGLU626418:GJI5-2579-MONOMER.

    Family and domain databases

    HAMAPi MF_01123. Ac_CoA_synth.
    InterProi IPR011904. Ac_CoA_lig.
    IPR025110. AMP-bd_C.
    IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view ]
    Pfami PF00501. AMP-binding. 1 hit.
    PF13193. AMP-binding_C. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
    PROSITEi PS00455. AMP_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of Burkholderia glumae BGR1."
      Lim J., Lee T.-H., Nahm B.H., Choi Y.D., Kim M., Hwang I.
      J. Bacteriol. 191:3758-3759(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: BGR1Imported.

    Entry informationi

    Entry nameiC5AA24_BURGB
    AccessioniPrimary (citable) accession number: C5AA24
    Entry historyi
    Integrated into UniProtKB/TrEMBL: July 28, 2009
    Last sequence update: July 28, 2009
    Last modified: October 1, 2014
    This is version 37 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3