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C5A701 (C5A701_THEGJ) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 29. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methionine--tRNA ligase HAMAP-Rule MF_00098
EC=6.1.1.10 HAMAP-Rule MF_00098
Alternative name(s):
Methionyl-tRNA synthetase HAMAP-Rule MF_00098
Gene names
Name:metG HAMAP-Rule MF_00098 EMBL ACS34013.1
Ordered Locus Names:TGAM_1511 EMBL ACS34013.1
OrganismThermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3) [Complete proteome] [HAMAP] EMBL ACS34013.1
Taxonomic identifier593117 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus ›

Protein attributes

Sequence length739 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP-Rule MF_00098

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP-Rule MF_00098

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00098

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00098

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00098.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily. HAMAP-Rule MF_00098

Contains 1 tRNA-binding domain. HAMAP-Rule MF_00098

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain639 – 739101tRNA-binding By similarity HAMAP-Rule MF_00098
Motif11 – 2111"HIGH" region By similarity HAMAP-Rule MF_00098
Motif344 – 3485"KMSKS" region By similarity HAMAP-Rule MF_00098

Sites

Metal binding1431Zinc By similarity HAMAP-Rule MF_00098
Metal binding1461Zinc By similarity HAMAP-Rule MF_00098
Metal binding1561Zinc By similarity HAMAP-Rule MF_00098
Metal binding1591Zinc By similarity HAMAP-Rule MF_00098
Binding site3471ATP By similarity HAMAP-Rule MF_00098

Sequences

Sequence LengthMass (Da)Tools
C5A701 [UniParc].

Last modified July 28, 2009. Version 1.
Checksum: EFF8FA6367405F33

FASTA73985,972
        10         20         30         40         50         60 
MVRYMVTSAL PYANGPIHAG HLAGAYLPAD IFVRYLRLKG EEVLFICGTD EHGTPITFRA 

        70         80         90        100        110        120 
LKEGRSPREI VDEFHEHIKT TFERAKISFD YFGRTELPVH YRISQEFFLK ALENGHLVKK 

       130        140        150        160        170        180 
VTKQAYCEHD KMFLPDRYVI GTCPYCGAEN QRGDQCEVCG RPLTPEILIN PRCNICGNPI 

       190        200        210        220        230        240 
TFKDSAHYYI KMQDFEERLK KWIESRPWKP NVKNTVLGWI NEGLEERAIT RDLDWGIPVP 

       250        260        270        280        290        300 
LDDEDMRGKV LYVWFEAPIG YISITIEALK RAGKEKEWKK FWLNLEGETR VIHFIGKDNI 

       310        320        330        340        350        360 
PFHAIFWPAF LMAYGKYKDE EVEADWNLPY DIPANEYLNL EGKKFSTSRN WAIWVHEFLD 

       370        380        390        400        410        420 
AFPADYLRYY LTAIMPETRD SDFSFADFKV KINEELVNNL GNFVHRALTF VNRYFDGIVP 

       430        440        450        460        470        480 
ERGELNELDK QAFEEIERAF EEVGELISQY RFKDALRRVM ELAIFGNRYF DHQRPWKTAK 

       490        500        510        520        530        540 
TDRKRTATTV NVSLQIVKAL GILLEPFLPD ASEKIWHLLN LEELKRWEFT EIPAGHRVRK 

       550        560        570        580        590        600 
ATPMFRKVTD EDIIYFILNY IARGNPESAK ILLDKYYKRD DVVKVALERF KNEEEARAIL 

       610        620        630        640        650        660 
KSVYGEELEV KSEKPGKASK KEKVKKKEGG KMDYVSFDDF AKLDLRVGKI IEVKDHPNAD 

       670        680        690        700        710        720 
RLYVVKVDLG DEVRQLVAGL KKYYKPEELL NKTVIVIANL EPKKLRGVES QGMLLAADDG 

       730 
ENVALLMPDK EVKLGARIR

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References

[1]"Genome analysis and genome-wide proteomics of Thermococcus gammatolerans, the most radioresistant organism known amongst the Archaea."
Zivanovic Y., Armengaud J., Lagorce A., Leplat C., Guerin P., Dutertre M., Anthouard V., Forterre P., Wincker P., Confalonieri F.
Genome Biol. 10:R70-R70(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 15229 / JCM 11827 / EJ3.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001398 Genomic DNA. Translation: ACS34013.1.
RefSeqYP_002959877.1. NC_012804.1.

3D structure databases

ProteinModelPortalC5A701.
ModBaseSearch...

Protein-protein interaction databases

STRING593117.TGAM_1511.
C5A701.

Proteomic databases

PaxDbC5A701.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACS34013; ACS34013; TGAM_1511.
GeneID7987313.
KEGGtga:TGAM_1511.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0143.
HOGENOMHOG000200402.
KOK01874.
OMAHEIAPFK.
ProtClustDBPRK00133.

Family and domain databases

Gene3D2.40.50.140. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00098. Met_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR004495. Met-tRNA-synth_Ia_bsu_C.
IPR023458. Met-tRNA_ligase_1.
IPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMsTIGR00398. metG. 1 hit.
TIGR00399. metG_C_term. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameC5A701_THEGJ
AccessionPrimary (citable) accession number: C5A701
Entry history
Integrated into UniProtKB/TrEMBL: July 28, 2009
Last sequence update: July 28, 2009
Last modified: May 1, 2013
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info