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Protein

DNA polymerase sliding clamp

Gene

pcn

Organism
Thermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Sliding clamp subunit that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase and other proteins to DNA during high-speed replication.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase sliding clampUniRule annotation
Alternative name(s):
Proliferating cell nuclear antigen homologUniRule annotation
Short name:
PCNAUniRule annotation
Gene namesi
Name:pcnUniRule annotation
Ordered Locus Names:TGAM_1046
OrganismiThermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3)
Taxonomic identifieri593117 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
Proteomesi
  • UP000001488 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002050821 – 249DNA polymerase sliding clampAdd BLAST249

Proteomic databases

PaxDbiC5A5N6.
PRIDEiC5A5N6.

Interactioni

Subunit structurei

Homotrimer. The subunits circularize to form a toroid; DNA passes through its center. Replication factor C (RFC) is required to load the toroid on the DNA.UniRule annotation

Protein-protein interaction databases

STRINGi593117.TGAM_1046.

Structurei

Secondary structure

1249
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Helixi10 – 21Combined sources12
Beta strandi25 – 31Combined sources7
Beta strandi33 – 41Combined sources9
Beta strandi47 – 54Combined sources8
Helixi55 – 57Combined sources3
Beta strandi58 – 63Combined sources6
Beta strandi67 – 72Combined sources6
Helixi73 – 80Combined sources8
Beta strandi88 – 93Combined sources6
Beta strandi95 – 114Combined sources20
Beta strandi132 – 137Combined sources6
Helixi138 – 149Combined sources12
Beta strandi153 – 159Combined sources7
Beta strandi161 – 169Combined sources9
Beta strandi174 – 180Combined sources7
Beta strandi186 – 193Combined sources8
Beta strandi195 – 200Combined sources6
Helixi201 – 208Combined sources8
Beta strandi216 – 221Combined sources6
Beta strandi227 – 233Combined sources7
Turni234 – 236Combined sources3
Beta strandi237 – 243Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5A6DX-ray2.80A/B1-249[»]
SMRiC5A5N6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PCNA family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00488. Archaea.
COG0592. LUCA.
HOGENOMiHOG000222371.
KOiK04802.
OMAiSDGFDKY.

Family and domain databases

HAMAPiMF_00317. DNApol_clamp_arch. 1 hit.
InterProiIPR000730. Pr_cel_nuc_antig.
IPR022649. Pr_cel_nuc_antig_C.
IPR022659. Pr_cel_nuc_antig_CS.
IPR022648. Pr_cel_nuc_antig_N.
[Graphical view]
PANTHERiPTHR11352. PTHR11352. 1 hit.
PfamiPF02747. PCNA_C. 1 hit.
PF00705. PCNA_N. 1 hit.
[Graphical view]
PRINTSiPR00339. PCNACYCLIN.
TIGRFAMsiTIGR00590. pcna. 1 hit.
PROSITEiPS01251. PCNA_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C5A5N6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFEIVFDGA KEFADLIATA SNLIDEAAFK ITEEGVSMRA MDPSRVVLID
60 70 80 90 100
LNLPESIFSK YEVEEPETIG INMDHFKKIL KRGKSKDTLI LRKGDENFLE
110 120 130 140 150
ITFEGTAKRT FRLPLIDVEE LELELPELPF TAKVVLLGEV LKEAIKDASL
160 170 180 190 200
VSDSLKFIAK EDEFTMKAEG ETNEVEIKLT LEDEGLLDLE VEEETRSAYG
210 220 230 240
ISYLADMVKG IGKADEVTLR FGTEMPLQMD YFIRDEGKLT FLLAPRVEE
Length:249
Mass (Da):28,125
Last modified:July 28, 2009 - v1
Checksum:i3320BE45B71FB1AB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001398 Genomic DNA. Translation: ACS33548.1.
RefSeqiWP_015858662.1. NC_012804.1.

Genome annotation databases

EnsemblBacteriaiACS33548; ACS33548; TGAM_1046.
GeneIDi7986920.
KEGGitga:TGAM_1046.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001398 Genomic DNA. Translation: ACS33548.1.
RefSeqiWP_015858662.1. NC_012804.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5A6DX-ray2.80A/B1-249[»]
SMRiC5A5N6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi593117.TGAM_1046.

Proteomic databases

PaxDbiC5A5N6.
PRIDEiC5A5N6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACS33548; ACS33548; TGAM_1046.
GeneIDi7986920.
KEGGitga:TGAM_1046.

Phylogenomic databases

eggNOGiarCOG00488. Archaea.
COG0592. LUCA.
HOGENOMiHOG000222371.
KOiK04802.
OMAiSDGFDKY.

Family and domain databases

HAMAPiMF_00317. DNApol_clamp_arch. 1 hit.
InterProiIPR000730. Pr_cel_nuc_antig.
IPR022649. Pr_cel_nuc_antig_C.
IPR022659. Pr_cel_nuc_antig_CS.
IPR022648. Pr_cel_nuc_antig_N.
[Graphical view]
PANTHERiPTHR11352. PTHR11352. 1 hit.
PfamiPF02747. PCNA_C. 1 hit.
PF00705. PCNA_N. 1 hit.
[Graphical view]
PRINTSiPR00339. PCNACYCLIN.
TIGRFAMsiTIGR00590. pcna. 1 hit.
PROSITEiPS01251. PCNA_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCNA_THEGJ
AccessioniPrimary (citable) accession number: C5A5N6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: November 2, 2016
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.