C5A4U6 (PURL_THEGJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	TGAM_0756

Organism

Thermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3) [Complete proteome] [HAMAP]

Taxonomic identifier

593117 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus

Protein attributes

Sequence length	712 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 712	712	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_1000206050
Regions
	Nucleotide binding	89 – 100	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	C5A4U6 [UniParc]. Last modified July 28, 2009. Version 1. Checksum: 3CAD6B054C617A83	FASTA	712	78,051
	10 20 30 40 50 60 MFPHEEKLIR ERLGREPNEL EWAMLEVMWS EHASYKSSRP WLKLLPTENE HVILGPGEDA 70 80 90 100 110 120 GIVKFDDETW VVVGIESHNH PSAVEPYGGA ATGVGGIVRD ILCMGARPIA LLDPIRFGPL 130 140 150 160 170 180 EKERNRYLFE YVVKGIADYG NRIGVPTVGG ETEFDESLDS YTLVNVACVG IMRPEHLVHS 190 200 210 220 230 240 YVTEAGLKLI LVGNRTGRDG VHGVTFASEE LSENTEEDRS AVQIPDPFTE KLLIEATLEA 250 260 270 280 290 300 VYTGRVKALK DLGGGGLTCA ASEMAGKKGF GAVIYADRVP LREPGMTPTE VMISESQERM 310 320 330 340 350 360 LFAVKPEDVE EIGRIFEEYE LEWTVVGETI EEPRFVVYWK GEKVADLPIE LLTEVPTIEW 370 380 390 400 410 420 ELKPYSAEGP VETPDVPFER AFDLVWGSPN ILSKRWVWEQ YDHEVQGRTV LKPGRDAAVL 430 440 450 460 470 480 KINDEYGLAF VADGNPNHSY LNPYQGAMGA VAEVVRNLVS VGAEPLALVD NLNFASPERP 490 500 510 520 530 540 EVYWSFAETV RGLADAARAF GLAYVSGNVS FYNEVVDRPI KPTPVVAGLG KVKLEAIPNG 550 560 570 580 590 600 GFEEGLLIGV VGLTKPELGG SELFARLGVE GGLAPRVNLE EEKANANGVL EAIRRGLVRA 610 620 630 640 650 660 VHDVSRGGLA VALAKMAVAG NTGFTADLSK VPSETTNPIE VAFSESHGRY IVAFPEENLE 670 680 690 700 710 ELKGLFKHFV IIGRTGGSDA VFLWNGWELL RKPVSELKAV HESLPMLLGE EE

References

[1]

"Genome analysis and genome-wide proteomics of Thermococcus gammatolerans, the most radioresistant organism known amongst the Archaea." Zivanovic Y., Armengaud J., Lagorce A., Leplat C., Guerin P., Dutertre M., Anthouard V., Forterre P., Wincker P., Confalonieri F. Genome Biol. 10:R70.1-R70.23(2007) [PubMed: 19558674] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 15229 / JCM 11827 / EJ3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001398 Genomic DNA. Translation: ACS33258.1.
RefSeq	YP_002959122.1. NC_012804.1.
3D structure databases
ProteinModelPortal	C5A4U6.
ModBase	Search...
Protein-protein interaction databases
STRING	C5A4U6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7987730.
GenomeReviews	Gene locus TGAM_0756 in contig CP001398_GR.
KEGG	tga:TGAM_0756.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	YGNSFGV.
ProtClustDB	PRK01213.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_THEGJ

Accession

Primary (citable) accession number: C5A4U6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	July 28, 2009
Last modified:	January 25, 2012
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program