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C5A4U0 (PUR2_THEGJ) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:TGAM_0750
OrganismThermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3) [Complete proteome] [HAMAP]
Taxonomic identifier593117 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus

Protein attributes

Sequence length438 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 438438Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_1000203243

Regions

Domain108 – 316209ATP-grasp
Nucleotide binding135 – 19460ATP By similarity

Sites

Metal binding2741Magnesium or manganese 1 By similarity
Metal binding2861Magnesium or manganese 1 By similarity
Metal binding2861Magnesium or manganese 2 By similarity
Metal binding2881Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
C5A4U0 [UniParc].

Last modified July 28, 2009. Version 1.
Checksum: 1C452A64EBBDFCE9

FASTA43848,267
        10         20         30         40         50         60 
MRVLLVGGGG REHAIGEALV RGGAELYVVS KHKNPGLARL ARGYGLAKET DIKKVLEYAE 

        70         80         90        100        110        120 
KFGVELAFIG PEAPLEKGIV DALEENGIPA VGPSREAAKL ETDKAFARTF MERNEIPGRK 

       130        140        150        160        170        180 
VFRVFTDVSK MRSWVDDFGR PVVVKPIGLT GGKGVKVVGY QLRDNEEAKS YAEELIRRDG 

       190        200        210        220        230        240 
RVLIEERTNG VEFTFQVFTD GKRVLPMPLA QDYPHAYEND EGPITGGMGS YSCSNGLLPF 

       250        260        270        280        290        300 
VTREDYEKAL ETLKATVEAM RKEGTPYKGI LYGQFMLSKG GPVLIEYNAR FGDPEAINVL 

       310        320        330        340        350        360 
PLLETSLLEV AEGIVDGNLQ GAEFEKKATV VKYLAPKGYP TNPVRGVKVE VNEKAVEEVG 

       370        380        390        400        410        420 
ARLYYASIDE NFTLLGSRAI AVVGIADSLE EAERIAKSVI PHVKGELFYR RDVGTRKSVE 

       430 
KRIELMKEFG KEFEPNPC 

« Hide

References

[1]"Genome analysis and genome-wide proteomics of Thermococcus gammatolerans, the most radioresistant organism known amongst the Archaea."
Zivanovic Y., Armengaud J., Lagorce A., Leplat C., Guerin P., Dutertre M., Anthouard V., Forterre P., Wincker P., Confalonieri F.
Genome Biol. 10:R70.1-R70.23(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 15229 / JCM 11827 / EJ3.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001398 Genomic DNA. Translation: ACS33252.1.
RefSeqYP_002959116.1. NC_012804.1.

3D structure databases

ProteinModelPortalC5A4U0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING593117.TGAM_0750.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACS33252; ACS33252; TGAM_0750.
GeneID7987724.
KEGGtga:TGAM_0750.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033464.
KOK01945.
OMADPEAMNV.
ProtClustDBPRK00885.

Enzyme and pathway databases

BioCycTGAM593117:GHFT-770-MONOMER.
UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_THEGJ
AccessionPrimary (citable) accession number: C5A4U0
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: February 19, 2014
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways