C5A4U0 (PUR2_THEGJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylamine--glycine ligase
EC=6.3.4.13

Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	TGAM_0750

Organism

Thermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3) [Complete proteome] [HAMAP]

Taxonomic identifier

593117 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus › Thermococcus gammatolerans

Protein attributes

Sequence length	438 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway purine nucleobase biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 438	438	Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138		PRO_1000203243
Regions
	Domain	108 – 316	209	ATP-grasp
	Nucleotide binding	135 – 194	60	ATP By similarity
Sites
	Metal binding	274	1	Magnesium or manganese 1 By similarity
	Metal binding	286	1	Magnesium or manganese 1 By similarity
	Metal binding	286	1	Magnesium or manganese 2 By similarity
	Metal binding	288	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	C5A4U0 [UniParc]. Last modified July 28, 2009. Version 1. Checksum: 1C452A64EBBDFCE9	FASTA	438	48,267
	10 20 30 40 50 60 MRVLLVGGGG REHAIGEALV RGGAELYVVS KHKNPGLARL ARGYGLAKET DIKKVLEYAE 70 80 90 100 110 120 KFGVELAFIG PEAPLEKGIV DALEENGIPA VGPSREAAKL ETDKAFARTF MERNEIPGRK 130 140 150 160 170 180 VFRVFTDVSK MRSWVDDFGR PVVVKPIGLT GGKGVKVVGY QLRDNEEAKS YAEELIRRDG 190 200 210 220 230 240 RVLIEERTNG VEFTFQVFTD GKRVLPMPLA QDYPHAYEND EGPITGGMGS YSCSNGLLPF 250 260 270 280 290 300 VTREDYEKAL ETLKATVEAM RKEGTPYKGI LYGQFMLSKG GPVLIEYNAR FGDPEAINVL 310 320 330 340 350 360 PLLETSLLEV AEGIVDGNLQ GAEFEKKATV VKYLAPKGYP TNPVRGVKVE VNEKAVEEVG 370 380 390 400 410 420 ARLYYASIDE NFTLLGSRAI AVVGIADSLE EAERIAKSVI PHVKGELFYR RDVGTRKSVE 430 KRIELMKEFG KEFEPNPC

References

[1]

"Genome analysis and genome-wide proteomics of Thermococcus gammatolerans, the most radioresistant organism known amongst the Archaea." Zivanovic Y., Armengaud J., Lagorce A., Leplat C., Guerin P., Dutertre M., Anthouard V., Forterre P., Wincker P., Confalonieri F. Genome Biol. 10:R70.1-R70.23(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 15229 / JCM 11827 / EJ3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001398 Genomic DNA. Translation: ACS33252.1.
RefSeq	YP_002959116.1. NC_012804.1.
3D structure databases
ProteinModelPortal	C5A4U0.
ModBase	Search...
Protein-protein interaction databases
STRING	593117.TGAM_0750.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACS33252; ACS33252; TGAM_0750.
GeneID	7987724.
KEGG	tga:TGAM_0750.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0151.
HOGENOM	HOG000033464.
KO	K01945.
OMA	MGAYTPL.
ProtClustDB	PRK00885.
Enzyme and pathway databases
UniPathway	UPA00074; UER00125.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 1 hit. 3.40.50.20. 1 hit. 3.90.600.10. 1 hit.
HAMAP	MF_00138. GARS.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR016185. PreATP-grasp_dom. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N. IPR011054. Rudment_hybrid_motif. [Graphical view]
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit. SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_THEGJ

Accession

Primary (citable) accession number: C5A4U0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	July 28, 2009
Last modified:	May 1, 2013
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program