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Protein

CCA-adding enzyme

Gene

cca

Organism
Thermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.UniRule annotation

Catalytic activityi

A tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei53 – 531ATP or CTPUniRule annotation
Binding sitei56 – 561ATP or CTPUniRule annotation
Metal bindingi65 – 651MagnesiumUniRule annotation
Metal bindingi67 – 671MagnesiumUniRule annotation
Metal bindingi119 – 1191MagnesiumUniRule annotation
Binding sitei142 – 1421ATP or CTPUniRule annotation
Binding sitei161 – 1611ATP or CTPUniRule annotation
Binding sitei170 – 1701ATP or CTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

RNA repair, tRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciTGAM593117:GHFT-430-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
CCA-adding enzymeUniRule annotation (EC:2.7.7.72UniRule annotation)
Alternative name(s):
CCA tRNA nucleotidyltransferaseUniRule annotation
tRNA CCA-pyrophosphorylaseUniRule annotation
tRNA adenylyl-/cytidylyl- transferaseUniRule annotation
tRNA nucleotidyltransferaseUniRule annotation
tRNA-NTUniRule annotation
Gene namesi
Name:ccaUniRule annotation
Ordered Locus Names:TGAM_0419
OrganismiThermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3)
Taxonomic identifieri593117 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
Proteomesi
  • UP000001488 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 454454CCA-adding enzymePRO_1000214146Add
BLAST

Proteomic databases

PaxDbiC5A3V9.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi593117.TGAM_0419.

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04249. Archaea.
COG1746. LUCA.
HOGENOMiHOG000226400.
KOiK07558.
OMAiDRTPFHN.

Family and domain databases

HAMAPiMF_01264. CCA_arch.
InterProiIPR008229. CCA-adding_arc.
IPR011068. NuclTrfase_I_C.
IPR002934. Polymerase_NTP_transf_dom.
IPR015329. tRNA_NucTransf2.
[Graphical view]
PfamiPF01909. NTP_transf_2. 1 hit.
PF09249. tRNA_NucTransf2. 1 hit.
[Graphical view]
PIRSFiPIRSF005335. CCA_arch. 1 hit.
SUPFAMiSSF55003. SSF55003. 1 hit.
TIGRFAMsiTIGR03671. cca_archaeal. 1 hit.

Sequencei

Sequence statusi: Complete.

C5A3V9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEMEEVLSEV IQRIRPSDEE RAFVKGLMEE LRTIAEERIE ELGLDARPYF
60 70 80 90 100
VGSLAKDTYL AGDHDIDLFL AFPLETPLEE LRKKGLELGK AIAEKLDSHE
110 120 130 140 150
IAYAEHPYVR ARYRGVRVDL VPCYDVGNWR DVRTAVDRSI LHTRWVNENL
160 170 180 190 200
RGRNDEVRLL KRFLKGINAY GSEIYVRGFS GYLAEILVIK YGSFLDVVEK
210 220 230 240 250
ADFLLRQKII DPENWLRKEP EVALKTVKRE TEEDRPLIVI DPVDPRRNVS
260 270 280 290 300
ANLSWEKYGR FYFKSIEFLE NPSVGFFFPP EKPKGSYLDE LRKRGTALVT
310 320 330 340 350
LLINVPDMVD DVLLPQLERS ARGFERTLER EGFGVLGWDV GRKGRAFIML
360 370 380 390 400
ELDRERRERV KIKPGPEFFT ERGRDFYRKN EKVWLIGKRL YSEKLVRESV
410 420 430 440 450
VDVIIELLEK NQVSLGKGIR DAIRRADILL NYVPKELEEE AYLFLSREKW

NIKG
Length:454
Mass (Da):53,071
Last modified:July 28, 2009 - v1
Checksum:i075AA82A53C95716
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001398 Genomic DNA. Translation: ACS32921.1.
RefSeqiWP_015858039.1. NC_012804.1.

Genome annotation databases

EnsemblBacteriaiACS32921; ACS32921; TGAM_0419.
GeneIDi7987966.
KEGGitga:TGAM_0419.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001398 Genomic DNA. Translation: ACS32921.1.
RefSeqiWP_015858039.1. NC_012804.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi593117.TGAM_0419.

Proteomic databases

PaxDbiC5A3V9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACS32921; ACS32921; TGAM_0419.
GeneIDi7987966.
KEGGitga:TGAM_0419.

Phylogenomic databases

eggNOGiarCOG04249. Archaea.
COG1746. LUCA.
HOGENOMiHOG000226400.
KOiK07558.
OMAiDRTPFHN.

Enzyme and pathway databases

BioCyciTGAM593117:GHFT-430-MONOMER.

Family and domain databases

HAMAPiMF_01264. CCA_arch.
InterProiIPR008229. CCA-adding_arc.
IPR011068. NuclTrfase_I_C.
IPR002934. Polymerase_NTP_transf_dom.
IPR015329. tRNA_NucTransf2.
[Graphical view]
PfamiPF01909. NTP_transf_2. 1 hit.
PF09249. tRNA_NucTransf2. 1 hit.
[Graphical view]
PIRSFiPIRSF005335. CCA_arch. 1 hit.
SUPFAMiSSF55003. SSF55003. 1 hit.
TIGRFAMsiTIGR03671. cca_archaeal. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome analysis and genome-wide proteomics of Thermococcus gammatolerans, the most radioresistant organism known amongst the Archaea."
    Zivanovic Y., Armengaud J., Lagorce A., Leplat C., Guerin P., Dutertre M., Anthouard V., Forterre P., Wincker P., Confalonieri F.
    Genome Biol. 10:R70.1-R70.23(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 15229 / JCM 11827 / EJ3.

Entry informationi

Entry nameiCCA_THEGJ
AccessioniPrimary (citable) accession number: C5A3V9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: March 16, 2016
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

A single active site specifically recognizes both ATP and CTP and is responsible for their addition.UniRule annotation

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.