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C5A3B6 (C5A3B6_THEGJ) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Transcription factor E HAMAP-Rule MF_01909
Short name=TFE HAMAP-Rule MF_01909
Alternative name(s):
TFIIE subunit alpha homolog HAMAP-Rule MF_01909
Transcription initiation factor TFIIE HAMAP-Rule MF_01909
Gene names
Name:tfe HAMAP-Rule MF_01909 EMBL ACS32728.1
Ordered Locus Names:TGAM_0226 EMBL ACS32728.1
OrganismThermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3) [Complete proteome] [HAMAP] EMBL ACS32728.1
Taxonomic identifier593117 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus ›

Protein attributes

Sequence length221 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Transcription factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Facilitates transcription initiation by enhancing TATA-box recognition by TATA-box-binding protein (Tbp), and transcription factor B (Tfb) and RNA polymerase recruitment. Not absolutely required for transcription in vitro, but particularly important in cases where Tbp or Tfb function is not optimal. It dynamically alters the nucleic acid-binding properties of RNA polymerases by stabilizing the initiation complex and destabilizing elongation complexes. Seems to translocate with the RNA polymerase following initiation and acts by binding to the non template strand of the transcription bubble in elongation complexes By similarity. HAMAP-Rule MF_01909

Subunit structure

Monomer. Interaction with RNA polymerase subunits RpoF and RpoE is necessary for Tfe stimulatory transcription activity. Able to interact with Tbp and RNA polymerase in the absence of DNA promoter. Interacts both with the preinitiation and elongation complexes By similarity. HAMAP-Rule MF_01909

Domain

The winged helix domain is involved in binding to DNA in the preinitiation complex By similarity. HAMAP-Rule MF_01909

Sequence similarities

Belongs to the TFE family. HAMAP-Rule MF_01909

Contains 1 HTH TFE/IIEalpha-type domain. HAMAP-Rule MF_01909 SAAS SAAS024550

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain9 – 10597HTH TFE/IIEalpha-type By similarity HAMAP-Rule MF_01909

Sequences

Sequence LengthMass (Da)Tools
C5A3B6 [UniParc].

Last modified July 28, 2009. Version 1.
Checksum: 9FF9E2CB67D53582

FASTA22125,815
        10         20         30         40         50         60 
MRPHRAPKIR AEVVIVARKK RLDKDLIDLA MEIGGEEAVE IVKALAERKD ATDEELAEST 

        70         80         90        100        110        120 
GIRINTVRKV LYTLYDMGLA DFKRIRDKET GWYYYYWNLD LRRLPDIIRA RKMEELKKLR 

       130        140        150        160        170        180 
KMLEEESGEI YYWCGNPGHP RLTFDEAMEY EFQCPICGEI LMQYDNSEII EDLKRRIEEL 

       190        200        210        220 
ELELGLKKSK KAKKSSRKSG KSSRSKSKKS SKTTSKAEVV A

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References

[1]"Genome analysis and genome-wide proteomics of Thermococcus gammatolerans, the most radioresistant organism known amongst the Archaea."
Zivanovic Y., Armengaud J., Lagorce A., Leplat C., Guerin P., Dutertre M., Anthouard V., Forterre P., Wincker P., Confalonieri F.
Genome Biol. 10:R70-R70(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 15229 / JCM 11827 / EJ3.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001398 Genomic DNA. Translation: ACS32728.1.
RefSeqYP_002958592.1. NC_012804.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRING593117.TGAM_0226.
C5A3B6.

Proteomic databases

PaxDbC5A3B6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACS32728; ACS32728; TGAM_0226.
GeneID7988801.
KEGGtga:TGAM_0226.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1675.
HOGENOMHOG000015477.
KOK03136.
OMAGWYYYYW.
ProtClustDBPRK06266.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
HAMAPMF_01909. TFE_arch.
InterProIPR016481. TF_E_archaea.
IPR017919. TFIIE/TFIIEa_HTH.
IPR002853. TFIIE_asu.
IPR024550. TFIIEa/SarR/Rpc3_HTH_dom.
IPR011991. WHTH_DNA-bd_dom.
IPR013137. Znf_TFIIB.
[Graphical view]
PfamPF08271. TF_Zn_Ribbon. 1 hit.
PF02002. TFIIE_alpha. 1 hit.
[Graphical view]
PIRSFPIRSF006373. TF_E_archaea. 1 hit.
SMARTSM00531. TFIIE. 1 hit.
[Graphical view]
TIGRFAMsTIGR00373. TIGR00373. 1 hit.
PROSITEPS51344. HTH_TFE_IIE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameC5A3B6_THEGJ
AccessionPrimary (citable) accession number: C5A3B6
Entry history
Integrated into UniProtKB/TrEMBL: July 28, 2009
Last sequence update: July 28, 2009
Last modified: May 1, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info