C5A2S3 (C5A2S3_THEGJ) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase HAMAP MF_00193
EC=6.3.1.5 HAMAP MF_00193

Gene names

Name:	nadE HAMAP MF_00193
Ordered Locus Names:	TGAM_0073

Organism

Thermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3) [Complete proteome] [HAMAP] EMBL ACS32575.1

Taxonomic identifier

593117 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family. HAMAP MF_00193

Ontologies

Keywords
Ligand	ATP-binding HAMAP MF_00193 NAD HAMAP MF_00193 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00193 EMBL ACS32575.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: HAMAP double-stranded DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	32 – 39	8	ATP By similarity HAMAP MF_00193
Sites
	Active site	34	1	By similarity HAMAP MF_00193

Sequences

	Sequence		Length	Mass (Da)
	C5A2S3 [UniParc]. Last modified July 28, 2009. Version 1. Checksum: 22050FBF7514F63D	FASTA	254	28,478
	10 20 30 40 50 60 MRELNYGEVI EKLVSFIQEK VGEAKSNGVV IGISGGVDSA TTAYLAVKAL GRERVLGLIM 70 80 90 100 110 120 PYYNNQDVDD ARLVCSSLGI ECREIGIKPI VDSFVAGLGF QPDKRSLGNI MSRTRMVLLY 130 140 150 160 170 180 AHANAKNYLV LGTGNRSEFL TGYFTKWGDG ASDYAPLINL YKTEVWEVAK LLGVPERIIE 190 200 210 220 230 240 KKPRAGLWEG QTDEDELGIS YRLLDEILWR LVDLKMPKEE IAGELGIPVE RVEYVENLVR 250 RSEHKRRLPL GPEF

References

[1]

"Genome analysis and genome-wide proteomics of Thermococcus gammatolerans, the most radioresistant organism known amongst the Archaea." Zivanovic Y., Armengaud J., Lagorce A., Leplat C., Guerin P., Dutertre M., Anthouard V., Forterre P., Wincker P., Confalonieri F. Genome Biol. 10:R70.1-R70.23(2007) [PubMed: 19558674] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 15229 / JCM 11827 / EJ3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001398 Genomic DNA. Translation: ACS32575.1.
RefSeq	YP_002958439.1. NC_012804.1.
3D structure databases
ProteinModelPortal	C5A2S3.
ModBase	Search...
Protein-protein interaction databases
STRING	C5A2S3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7988234.
GenomeReviews	Gene locus TGAM_0073 in contig CP001398_GR.
KEGG	tga:TGAM_0073.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	IDERIVS.
ProtClustDB	PRK13980.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. IPR018126. SASP_alpha/beta-type_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
PROSITE	PS00304. SASP_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C5A2S3_THEGJ

Accession

Primary (citable) accession number: C5A2S3

Entry history

Integrated into UniProtKB/TrEMBL:	July 28, 2009
Last sequence update:	July 28, 2009
Last modified:	December 14, 2011
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)