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C5A2D9

- ENDA_THEGJ

UniProt

C5A2D9 - ENDA_THEGJ

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Protein
tRNA-splicing endonuclease
Gene
endA, TGAM_2056
Organism
Thermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3)
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp By similarity.UniRule annotation

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei107 – 1071 By similarity
Active sitei114 – 1141 By similarity
Active sitei145 – 1451 By similarity

GO - Molecular functioni

  1. lyase activity Source: UniProtKB-KW
  2. nucleic acid binding Source: InterPro
  3. tRNA-intron endonuclease activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciTGAM593117:GHFT-2109-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonuclease (EC:4.6.1.16)
Alternative name(s):
tRNA-intron endonuclease
Gene namesi
Name:endA
Ordered Locus Names:TGAM_2056
OrganismiThermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3)
Taxonomic identifieri593117 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
ProteomesiUP000001488: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 168168tRNA-splicing endonucleaseUniRule annotation
PRO_1000216085Add
BLAST

Proteomic databases

PaxDbiC5A2D9.

Interactioni

Subunit structurei

Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers By similarity.

Protein-protein interaction databases

STRINGi593117.TGAM_2056.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1676.
HOGENOMiHOG000107823.
KOiK01170.
OMAiALKFGSH.

Family and domain databases

Gene3Di3.40.1350.10. 1 hit.
HAMAPiMF_01833. EndA_short.
InterProiIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR016442. tRNA_splic_arch_short.
[Graphical view]
PfamiPF01974. tRNA_int_endo. 1 hit.
PF02778. tRNA_int_endo_N. 1 hit.
[Graphical view]
PIRSFiPIRSF005285. tRNA_splic_archaea. 1 hit.
SUPFAMiSSF53032. SSF53032. 1 hit.
SSF55267. SSF55267. 1 hit.
TIGRFAMsiTIGR00324. endA. 1 hit.

Sequencei

Sequence statusi: Complete.

C5A2D9-1 [UniParc]FASTAAdd to Basket

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MIVFYLSGDR VFSTDQNAIN GLYNKRHFGK LVEGKLFLSL LEATYLVERG    50
KIEVREGKRK LTVEELMNLG RERDELFDAK YLVYKDLRDR GYTVKSGLKF 100
GSHFRVYRRG MDEHSQWLVW VLPENSRLSP NDITARVRVA HGVRKNMIMA 150
IVDEDADVTY YKVEWVRF 168
Length:168
Mass (Da):19,800
Last modified:July 28, 2009 - v1
Checksum:i5BF709EBC3F8C52C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001398 Genomic DNA. Translation: ACS34558.1.
RefSeqiWP_015859661.1. NC_012804.1.
YP_002960422.1. NC_012804.1.

Genome annotation databases

EnsemblBacteriaiACS34558; ACS34558; TGAM_2056.
GeneIDi7988622.
KEGGitga:TGAM_2056.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001398 Genomic DNA. Translation: ACS34558.1 .
RefSeqi WP_015859661.1. NC_012804.1.
YP_002960422.1. NC_012804.1.

3D structure databases

ModBasei Search...

Protein-protein interaction databases

STRINGi 593117.TGAM_2056.

Proteomic databases

PaxDbi C5A2D9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACS34558 ; ACS34558 ; TGAM_2056 .
GeneIDi 7988622.
KEGGi tga:TGAM_2056.

Phylogenomic databases

eggNOGi COG1676.
HOGENOMi HOG000107823.
KOi K01170.
OMAi ALKFGSH.

Enzyme and pathway databases

BioCyci TGAM593117:GHFT-2109-MONOMER.

Family and domain databases

Gene3Di 3.40.1350.10. 1 hit.
HAMAPi MF_01833. EndA_short.
InterProi IPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR016442. tRNA_splic_arch_short.
[Graphical view ]
Pfami PF01974. tRNA_int_endo. 1 hit.
PF02778. tRNA_int_endo_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF005285. tRNA_splic_archaea. 1 hit.
SUPFAMi SSF53032. SSF53032. 1 hit.
SSF55267. SSF55267. 1 hit.
TIGRFAMsi TIGR00324. endA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome analysis and genome-wide proteomics of Thermococcus gammatolerans, the most radioresistant organism known amongst the Archaea."
    Zivanovic Y., Armengaud J., Lagorce A., Leplat C., Guerin P., Dutertre M., Anthouard V., Forterre P., Wincker P., Confalonieri F.
    Genome Biol. 10:R70.1-R70.23(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 15229 / JCM 11827 / EJ3.

Entry informationi

Entry nameiENDA_THEGJ
AccessioniPrimary (citable) accession number: C5A2D9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: September 3, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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